Consulta: creatorFacets:"MANGARAVITE,Érica"
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Data/hora: 10/06/2024 10:08:43

A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment
Provedor de dados: 36
Autores: MANGARAVITE,Érica; TERRA,Vanessa; HATTORI,Eric Koiti Okiyama; DAL’SASSO,Thaís Carolina da Silva; BHERING,Leonardo Lopes; OLIVEIRA,Luiz Orlando de.
ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Ascorbic acid; Dithiothreitol; DNA isolation.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400335