Consulta: creatorFacets:"Macagnan,Marisa"
Registros recuperados: 2
Data/hora: 03/06/2024 01:18:13

Laryngotracheitis: reproducibility of the disease and comparison of diagnostic methods
Provedor de dados: Brazilian Journal of Microbiology
Autores: Beltrão,Nilzane; Furian,Thales Quedi; Souza,Guilherme Fonseca de; Macagnan,Marisa; Fallavena,Luiz César Bello; Canal,Claudio Wageck.
Infectious laryngotracheitis virus (ILTV) cause mild to severe respiratory disease in chickens, the purpose of our study being to use Brazilian isolate of ILTV to reproduce ILTV disease in chickens by experimental infection and to compare three diagnostic methods (nested polymerase chain reaction (PCR), virus isolation, histopathology) for detection of ILTV. Forty-eight chickens intratracheally inoculated with ILTV and a further 48 with PBS, showing mild respiratory signs 48 hours post infection (PI) but no signs of infection after day 10 PI. Every 2 days PI, six birds were arbitrarily selected from the control and infected groups, sacrificed and the trachea collected. Both the nested PCR and virus isolation detected the virus from day 2 until day 12 PI....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious laryngotracheitis virus; Avian pathology; Diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500024

Detection of Salmonella sp. from porcine origin: a comparison between a PCR method and standard microbiological techniques
Provedor de dados: Brazilian Journal of Microbiology
Autores: Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa.
The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection; PCR; Pork; Salmonella; Swine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013