Consulta: creatorFacets:"Macedo,Carla Isabel"
Registros recuperados: 2
Data/hora: 10/06/2024 07:55:25

Short-interfering RNAs as antivirals against rabies
Provedor de dados: 55
Autores: Brandão,Paulo Eduardo; Castilho,Juliana Galera; Fahl,Willian; Carnieli Jr.,Pedro; Oliveira,Rafael de Novaes; Macedo,Carla Isabel; Carrieri,Maria Luiza; Kotait,Ivanete.
This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SiRNA; Rabies; Antiviral.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200011

Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples
Provedor de dados: 55
Autores: Macedo,Carla Isabel; Carnieli Jr,Pedro; Brandão,Paulo Eduardo; Rosa,Elizabeth S. Travassos da; Oliveira,Rafael de Novaes; Castilho,Juliana Galera; Medeiros,Rita; Machado,Rosangela Rocha; Oliveira,Rosely Cerqueira de; Carrieri,Maria Luiza; Kotait,Ivanete.
Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human rabies; Neck-skin; Diagnosis; RT-PCR; DNA sequencing.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008