Consulta: creatorFacets:"Magnani,Marciane"
Registros recuperados: 10
Data/hora: 11/06/2024 06:40:55

Protective effect of carboxymethyl-glucan (CM-G) against DNA damage in patients with advanced prostate cancer
Provedor de dados: 74
Autores: Magnani,Marciane; Castro-Gomez,Raul Jorge Hernan; Mori,Mateus Prates; Kuasne,Hellen; Gregório,Emerson Pereira; Libos Jr,Farid; Cólus,Ilce Mara de Syllos.
Carboxymethyl-glucan (CM-G) is a soluble derivative from Saccharomyces cerevisiae (1 → 3)(1 → 6)-β-D-glucan. The protective efficiency of CM-G against DNA damage in cells from patients with advanced prostate cancer (PCa), and undergoing Androgen Deprivation Therapy (ADT), was evaluated. DNA damage scores were obtained by the comet assay, both before and after treatment with CM-G. The reduction in DNA damage, ranging from 18% to 87%, with an average of 59%, was not related to the increased number of leukocytes in peripheral blood. The results demonstrate for the first time the protective effect of CM-G against DNA damage in patients with advanced PCa. Among smokers, three presented the highest reduction in DNA damage after treatment with CM-G. There was no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Β-D-glucan; Carboxymethyl-glucan; Prostate cancer.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100023

Molecular identification of Aspergillus spp. isolated from coffee beans
Provedor de dados: 63
Autores: Magnani,Marciane; Fernandes,Thiago; Prete,Cássio Egidio Cavenaghi; Homechim,Martin; Ono,Elisabete Yurie Sataque; Vilas-Boas,Laurival Antonio; Sartori,Daniele; Furlaneto,Márcia Cristina; Fungaro,Maria Helena Pelegrinelli.
Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ITS; Ribosomal DNA; Ochratoxin; Mycotoxin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100009

Expression of the marA, soxS, acrB and ramA genes related to the AcrAB/TolC efflux pump in Salmonella entérica strains with and without quinolone resistance-determining regions gyrA gene mutations
Provedor de dados: 55
Autores: Ferrari,Rafaela Gomes; Galiana,Antonio; Cremades,Rosa; Rodríguez,Juan Carlos; Magnani,Marciane; Tognim,Maria Cristina Bronharo; Oliveira,Tereza C.R.M.; Royo,Gloria.
Several studies have been conducted in recent years to elucidate the structure, function and significance of AcrB, MarA, SoxS and RamA in Salmonella enterica. In this study, the relative quantification of acrB, soxS, marA and ramA genes expression was evaluated in 14 strains of S. enterica, with or without accompanying mutations in the quinolone resistance-determining regions of the gyrA gene, that were exposed to ciprofloxacin during the exponential growth phase. The presence of ciprofloxacin during the log phase of bacterial growth activated the genes marA, soxS, ramA and acrB in all S. enterica strains analyzed in this study. The highest expression levels for acrB were observed in strains with gyrA mutation, and marA showed the highest expression in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella sp.; Fluoroquinolone; Efflux pump; Antibiotic resistance.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200002

Plasmid-mediated quinolone resistance (PMQR) and mutations in the topoisomerase genes of Salmonella enterica strains from Brazil
Provedor de dados: 58
Autores: Ferrari,Rafaela; Galiana,Antonio; Cremades,Rosa; Rodríguez,Juan Carlos; Magnani,Marciane; Tognim,M.C.B.; Oliveira,Tereza C.R.M.; Royo,Gloria.
The objective of this study was to identify mutations in the Quinolone Resistance Determining sources Regions (QRDR) of the gyrA, gyrB, parC, and parE genes and to determine if any of the qnr variants or the aac(6')-Ib-cr variant were present in strains of Salmonella spp. isolated in Brazil. A total of 126 Salmonella spp. strains from epidemic (n = 114) and poultry (n = 12) origin were evaluated. One hundred and twelve strains (88.8%) were resistant to nalidixic acid (NAL) and 29 (23.01%) showed a reduced susceptibility to ciprofloxacin (Cip). The mutations identified were substitutions limited to the QRDR of the gyrA gene in the codons for Serine 83, Aspartate 87 and Alanine 131. The sensitivity to NAL seems to be a good phenotypic indication of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; PMQR; QRDR mutation; Quinolone; Ciprofloxacin.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200046

Habituation of enterotoxigenic Staphylococcus aureus to Origanum vulgare L. essential oil does not induce direct-tolerance and cross-tolerance to salts and organic acids
Provedor de dados: 58
Autores: Tavares,Adassa Gama; Monte,Daniel Farias Marinho do; Albuquerque,Allan dos Reis; Sampaio,Fábio Correia; Magnani,Marciane; Siqueira Júnior,José Pinto de; Souza,Evandro Leite de.
Enterotoxigenic Staphylococcus aureus strains that were isolated from foods were investigated for their ability to develop direct-tolerance and cross-tolerance to sodium chloride (NaCl), potassium chloride (KCl), lactic acid (LA) and acetic acid (AA) after habituation in sublethal amounts (1/2 of the minimum inhibitory concentration - 1/2 MIC and 1/4 of the minimum inhibitory concentration - 1/4 MIC) of Origanum vulgare L. essential oil (OVEO). The habituation of S. aureus to 1/2 MIC and 1/4 MIC of OVEO did not induce direct-tolerance or cross-tolerance in the tested strains, as assessed by modulation of MIC values. Otherwise, exposing the strains to OVEO at sublethal concentrations maintained or increased the sensitivity of the cells to the tested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus; Adaptation; Essential oil; Oregano.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300835

Detection of quinolone-resistance mutations in Salmonella spp. strains of epidemic and poultry origin
Provedor de dados: 58
Autores: Souza,Roberta Barreiros de; Magnani,Marciane; Ferrari,Rafaela Gomes; Kottwitz,Luciana Bill Mikito; Sartori,Daniele; Tognim,Maria Cristina Bronharo; Oliveira,Tereza Cristina R. M. de.
Mutations into codons Aspartate-87 (62%) and Serine-83 (38%) in QRDR of gyrA were identified in 105 Salmonella strains resistant to nalidixic acid (94 epidemic and 11 of poultry origin). The results show a high incidence of mutations associated to quinolone resistance but suggest association with others mechanisms of resistance.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quinolones; GyrA; Quinolone Resistance Determining Region (QRDR); AS-PCR-RFLP.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100026

Commercially laid eggs vs. discarded hatching eggs: contamination by Salmonella spp
Provedor de dados: 58
Autores: Kottwitz,Luciana B.M.; Leão,Joice Aparecida; Back,Alberto; Rodrigues,Dalia dos P.; Magnani,Marciane; Oliveira,Tereza C.R.M. de.
Salmonella enterica is frequently associated with outbreaks of human salmonellosis, and products of avian origin, such as eggs and chicken meat, are the main vehicles of its transmission. The present study describes the occurrence of different serovars of Salmonella enterica and phagotypes of S. enterica serovar Enteritidis in eggs destined for human consumption. Four thousand eggs obtained from commercial egg laying farms and one thousand discarded hatching eggs from broiler farms, which were acquired at farmers' markets and informal shops, were analyzed. Salmonella spp. was isolated from 52.0% of the discarded hatching eggs, in which the predominant serovar was Enteritidis (84.6%), and the predominant Salmonella Enteritidis phagotype (PT) was PT7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Discarded eggs; Salmonella Enteritidis; Antimicrobial resistance; Salmonellosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200004

Ciprofloxacin susceptibility reduction of Salmonella strains isolated from outbreaks
Provedor de dados: 58
Autores: Souza,Roberta B.; Ferrari,Rafaela G.; Magnani,Marciane; Kottwitz,Luciana B. M.; Alcocer,Iliana; Tognim,Maria Cristina B.; Oliveira,Tereza C. R. M.
The antimicrobial susceptibility of 212 Salmonella strains isolated from patients and foods was evaluated and 45% were found to be resistant to nalidixic acid. Nalidixic acid resistant strains showed a higher minimal inhibitory concentration for ciprofloxacin than sensitive strains. During the study an increase of strains with reduced susceptibility to ciprofloxacin was also observed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial resistance; Minimal Inhibitory Concentration (MIC); Quinolones; Fluoroquinolones.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200033

Influence of lipids and proteins amounts and pH values on the inhibitory effects of Origanum vulgare L. essential oil against Escherichia coli and Salmonella Typhimurium
Provedor de dados: 60
Autores: Medeiros,Andrea Sulamita de Jesus; Carvalho,Rhayane Idalyne; Conceição,Maria Lúcia da; Magnani,Marciane; Souza,Evandro Leite de.
Origanum vulgare L. (OVEO) essential oil has been considered a candidate antimicrobial for use in food conservation systems. However, studies on the influence of concomitant variations of different food components or physicochemical parameters on the antibacterial properties of OVEO are scarce. This study assessed the influence of concomitant variations in amounts of proteins - PTN (4.0, 6.0 or 8.0 g/100 mL) and lipids - LIP (3.75, 5.0 or 6.25 g/100 mL) and pH values (5.0, 5.5 or 6.0) in cultivation medium on the inhibitory effects of OVEO against Escherichia coli (EC) and Salmonella Typhimurium (ST). Lowest minimum inhibitory concentration values of OVEO against EC and ST were observed in media with the highest LIP amounts regardless the PTN amount and pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oregano; Antibacterial effects; Influential factors; Foodborne pathogens.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100622

Standardization of sodium metabisulfite solution concentrations and immersion time for farmed shrimp Litopenaeus vannamei
Provedor de dados: 65
Autores: Andrade,Luciana Trigueiro de; Araújo,Nkarthe Guerra; Ventura,Ana Paula Moraes; Lira,Andréa de Lucena; Magnani,Marciane; Cavalheiro,José Marcelino de Oliveira.
Sodium metabisulfite is the main additive used in the prevention of melanosis in shrimp; however, it has currently been employed with great variation in concentration by producers. Thus, the aim of the present study was to determine the correlation between the concentration of the sodium metabisulfite solution and immersion time of the whole shrimp to obtain the concentration of sulfur dioxide (SO2) in the edible muscle of farmed shrimp (Litopenaeus vannamei) in accordance with the limit established by law. For this, solutions of sodium metabisulfite at different concentrations (1%, 2 %, 3 %, 4% and 5%) were prepared and samples of L. vannamei shrimp (100g) were immersed during 10, 20 or 30 minutes at temperature of 7°C. For all treatment assayed the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sulfur dioxide; Edible muscle; Farmed shrimp.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000300499