Consulta: creatorFacets:"Manjula,S."
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Differential expression of metallothionein type-2 homologues in leaves and roots of Black pepper (Piper nigrum L)
Provedor de dados: Genetics and Molecular Biology
Autores: Alex,Susan M.; Dicto,Jose; Purushothama,M.G.; Manjula,S.
Black pepper (Piper nigrum L.), member of the family Piperaceae is indigenous to India and is one of the most widely used spices in the world. In this paper we report the results of our attempts to identify a set of genes differentially expressed in the leaves of Piper nigrum, which could facilitate targeted engineering of this valuable crop. A PCR-based Suppression Subtractive Hybridization (SSH) technique was used to generate a leaf-specific subtracted cDNA library of Piper nigrum. A tester population of leaf cDNA was subtracted with a root derived driver cDNA. The efficiency of subtraction was confirmed by PCR analysis using the housekeeping gene actin. On sequence analysis, almost 30% of the clones showed homology to metallothionein type-2 gene. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piper nigrum; Suppression subtractive hybridization; Metallothionein type-2; Real-time PCR analysis; Northern blotting.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300024