Consulta: creatorFacets:"Margis,Rogério"
Registros recuperados: 11
Data/hora: 10/06/2024 11:12:04

Identification of novel and conserved microRNAs in Coffea canephora and Coffea arabica
Provedor de dados: 74
Autores: Loss-Morais,Guilherme; Ferreira,Daniela C.R.; Margis,Rogério; Alves-Ferreira,Márcio; Corrêa,Régis L.
As microRNAs (miRNAs) are important regulators of many biological processes, a series of small RNAomes from plants have been produced in the last decade. However, miRNA data from several groups of plants are still lacking, including some economically important crops. Here microRNAs from Coffea canephora leaves were profiled and 58 unique sequences belonging to 33 families were found, including two novel microRNAs that have never been described before in plants. Some of the microRNA sequences were also identified in Coffea arabica that, together with C. canephora, correspond to the two major sources of coffee production in the world. The targets of almost all miRNAs were also predicted on coffee expressed sequences. This is the first report of novel miRNAs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; MicroRNA profiling; Illumina sequencing.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500010

Unravelling MADS-box gene family in Eucalyptus spp.: a starting point to an understanding of their developmental role in trees
Provedor de dados: 74
Autores: Dias,Beatriz Fonseca de Oliveira; Simões-Araújo,Jean Luiz; Russo,Claudia A.M.; Margis,Rogério; Alves-Ferreira,Márcio.
MADS-box genes encode a family of transcription factors which control diverse developmental processes in flowering plants ranging from root to flower and fruit development. Members of the MADS-box gene family share a highly conserved sequence of approximately 180 nucleotides that encodes a DNA-binding domain. We used bioinformatics tools to investigate the information generated by the Eucalyptus Expressed Sequence Tag (FORESTs) genome project in order to identify and annotate MADS-box genes. The comparative phylogenetic analysis of the Eucalyptus MADS-box genes with Arabidopsis homologues allowed us to group them into one of the well-known subfamilies. Trends in gene expression of these putative Eucalyptus MADS-box genes were investigated by hierarchical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MADS-box; Vegetative development; Reproductive development; Phylogenetic analysis; ESTs.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400004

Analysis of castor bean ribosome-inactivating proteins and their gene expression during seed development
Provedor de dados: 74
Autores: Loss-Morais,Guilherme; Turchetto-Zolet,Andreia Carina; Etges,Matheus; Cagliari,Alexandro; Körbes,Ana Paula; Maraschin,Felipe dos Santos; Margis-Pinheiro,Márcia; Margis,Rogério.
Ribosome-inactivating proteins (RIPs) are enzymes that inhibit protein synthesis after depurination of a specific adenine in rRNA. The RIP family members are classified as type I RIPs that contain an RNA-N-glycosidase domain and type II RIPs that contain a lectin domain (B chain) in addition to the glycosidase domain (A chain). In this work, we identified 30 new plant RIPs and characterized 18 Ricinus communis RIPs. Phylogenetic and functional divergence analyses indicated that the emergence of type I and II RIPs probably occurred before the monocot/eudicot split. We also report the expression profiles of 18 castor bean genes, including those for ricin and agglutinin, in five seed stages as assessed by quantitative PCR. Ricin and agglutinin were the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agglutinin; Evolution; Lipase; Ricinus communis; Ricin; RIPs.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100011

Identification, classification and expression pattern analysis of sugarcane cysteine proteinases
Provedor de dados: 74
Autores: Correa,Gustavo Coelho; Margis-Pinheiro,Márcia; Margis,Rogério.
Cysteine proteases are peptidyl hydrolyses dependent on a cysteine residue at the active center. The physical and chemical properties of cysteine proteases have been extensively characterized, but their precise biological functions have not yet been completely understood, although it is known that they are involved in a number of events such as protein turnover, cancer, germination, programmed cell death and senescence. Protein sequences from different cysteine proteinases, classified as members of the E.C.3.4.22 sub-sub-class, were used to perform a T-BLAST-n search on the Brazilian Sugarcane Expressed Sequence Tags project (SUCEST) data bank. Sequence homology was found with 76 cluster sequences that corresponded to possible cysteine proteinases. The...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100036

Patterns of genetic diversity in southern and southeastern Araucaria angustifolia (Bert.) O. Kuntze relict populations
Provedor de dados: 74
Autores: Souza,Maria Isabel Ferreira de; Salgueiro,Fabiano; Carnavale-Bottino,Mariana; Félix,Durvalina Benedita; Alves-Ferreira,Marcio; Bittencourt,Juliana Vitoria Messias; Margis,Rogério.
Habitat fragmentation and a decrease in population size may lead to a loss in population genetic diversity. For the first time, the reduction in genetic diversity in the northernmost limit of natural occurence (southeastern Brazil) of Araucaria angustifolia in comparison with populations in the main area of the species continuous natural distribution (southern Brazil), was tested. The 673 AFLPs markers revealed a high level of genetic diversity for the species (Ht = 0.27), despite anthropogenic influence throughout the last century, and a decrease of H in isolated populations of southeastern Brazil (H = 0.16), thereby indicating the tendency for higher genetic diversity in remnant populations of continuous forests in southern Brazil, when compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucariaceae; Relict populations; Araucaria forest; Population genetics; AFLP.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300018

Survey of glycine-rich proteins (GRPs) in the Eucalyptus expressed sequence tag database (ForEST)
Provedor de dados: 74
Autores: Bocca,Silvia Nora; Magioli,Claudia; Mangeon,Amanda; Junqueira,Ricardo Magrani; Cardeal,Vanessa; Margis,Rogério; Sachetto-Martins,Gilberto.
The occurrence of quasi-repetitive glycine-rich peptides has been reported in different organisms. Glycine-rich regions are proposed to be involved in protein-protein interactions in some mammalian protein families. In plants, a set of glycine-rich proteins (GRPs) was characterized several years ago, and since then a wealth of new GRPs have been identified. GRPs may have very diverse sub-cellular localization and functions. The only common feature among all different GRPs is the presence of glycine-rich repeat domains. The expression of genes encoding GRPs is developmentally regulated, and also induced, in several plant genera, by physical, chemical and biological factors. In addition to the highly modulated expression, several GRPs also show...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine-rich; GRP; Eucalyptus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400016

Sugarcane phytocystatins: Identification, classification and expression pattern analysis
Provedor de dados: 74
Autores: Reis,Emerson Moreira; Margis,Rogério.
The cystatins are tightly binding, but reversible, inhibitors of cysteine proteinases, which constitute a superfamily of evolutionary related proteins. They have been subdivided into three families: the cystatin family which contain two disulfide bonds, the stefin family which lack disulfide bonds, and the kininogen family composed of large glycoproteins containing three repeats similar to those found in the cystatin family. Members of the cystatin superfamily occurring in plants are currently known as phytocystatins, defined as proteins lacking disulfide bonds but possessing a conserved N-terminal amino acid sequence (L-A-R-[FY]-A-[VI]-X(3)-N). We have used the protein sequence deduced from seven phytocystatins (from the Arabidopsis thaliana genome...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100038

Classification, expression pattern and comparative analysis of sugarcane expressed sequences tags (ESTs) encoding glycine-rich proteins (GRPs)
Provedor de dados: 74
Autores: Fusaro,Adriana; Mangeon,Amanda; Junqueira,Ricardo Magrani; Rocha,Carla Andréa Benício; Coutinho,Tatiana Cardoso; Margis,Rogério; Sachetto-Martins,Gilberto.
Since the isolation of the first glycine-rich proteins (GRPs) in plants a wealth of new GRPs have been identified. The highly specific but diverse expression pattern of grp genes, taken together with the distinct sub-cellular localization of some GRP groups, clearly indicate that these proteins are involved in several independent physiological processes. Notwithstanding the absence of a clear definition of the role of GRPs in plant cells, studies conducted with these proteins have provided new and interesting insights into the molecular biology and cell biology of plants. Complexly regulated promoters and distinct mechanisms for the regulation of gene expression have been demonstrated and new protein targeting pathways, as well as the exportation of GRPs...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100035

AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
Provedor de dados: 74
Autores: Gerhardt,Liliane B. de A.; Magioli,Cláudia; Perez,Ana B.U.C.M.; Margis,Rogério; Sachetto-Martins,Gilberto; Margis-Pinheiro,Márcia.
The expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV promoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Chia4 Arabidopsis thaliana endochitinase; Embryogenesis; Gene expression; Promoter.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020

Salt stress affects mRNA editing in soybean chloroplasts
Provedor de dados: 74
Autores: Rodrigues,Nureyev F.; Fonseca,Guilherme C. da; Kulcheski,Franceli R.; Margis,Rogério.
Abstract Soybean, a crop known by its economic and nutritional importance, has been the subject of several studies that assess the impact and the effective plant responses to abiotic stresses. Salt stress is one of the main environmental stresses and negatively impacts crop growth and yield. In this work, the RNA editing process in the chloroplast of soybean plants was evaluated in response to a salt stress. Bioinformatics approach using sRNA and mRNA libraries were employed to detect specific sites showing differences in editing efficiency. RT-qPCR was used to measure editing efficiency at selected sites. We observed that transcripts of NDHA, NDHB, RPS14 and RPS16 genes presented differences in coverage and editing rates between control and salt-treated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Small RNA; Chloroplast; RNA editing; PPR; Salt stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200200

Genetic differentiation between Plectranthus grandis cramer and Plectranthus barbatus andr. (Lamiaceae)
Provedor de dados: 42
Autores: Passinho,Helna Célia R.; Margis,Rogério; Félix,Durvalina; Maia,Carla S.; Kaplan,Maria Auxiliadora C.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000200019