Consulta: creatorFacets:"Marins,Luis Fernando"
Registros recuperados: 4
Data/hora: 16/06/2024 19:16:01

Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy
Provedor de dados: 74
Autores: Figueiredo,Márcio de Azevedo; Lanes,Carlos Frederico Ceccon; Almeida,Daniela Volcan; Marins,Luis Fernando.
In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to descendants is difficult or impossible. We evaluated the degree of in vivo mosaicism using a strategy where a reporter transgene is co-injected with a transgene of interest so that potential germline founders can be easily identified. Transgenic zebrafish (Danio rerio) were produced using two transgenes, both comprised of the carp beta-actin promoter driving the expression of either the green fluorescent protein (GFP) reporter gene or the growth hormone cDNA from the marine silverside fish Odonthestes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenesis; Genetically modified fish; Microinjection; Growth hormone cDNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100008

Simultaneous overexpression of GH and STAT5b genes inhibits the STAT5 signalling pathway in tilapia (Oreochromis niloticus) embryos
Provedor de dados: 74
Autores: Marins,Luis Fernando; Iyengar,Arati; Maclean,Norman; Levy,Jose A.; Sohm,Frédéric.
In this study, we describe the use of a STAT5 responsive element (LHRE) reporter gene to monitor the activity of the growth hormone (GH) transduction pathway following expression of heterologous fish GH and rat STAT5b in tilapia embryos and fish fibroblast cells. Our results indicate that both GH and STAT5b are able to activate the LHRE at high levels when transferred separately, demonstrating the substantial level of conservation of the GH signal transduction pathways between fish and mammals. Unexpectedly, co-expression experiments show a strong inhibition of the GH-dependent activation, suggesting that simultaneous GH and STAT5b overexpression can counteract effects of GH expression in tilapia embryos.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teleost; Growth hormone; Signal transduction; Cytokine; Trangenesis signal transducers and activators of transcription.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300007

Green turtles (Chelonia mydas) foraging at Arvoredo Island in Southern Brazil: genetic characterization and mixed stock analysis through mtDNA control region haplotypes
Provedor de dados: 74
Autores: Proietti,Maíra Carneiro; Lara-Ruiz,Paula; Reisser,Júlia Wiener; Pinto,Luciano da Silva; Dellagostin,Odir Antonio; Marins,Luis Fernando.
We analyzed mtDNA control region sequences of green turtles (Chelonia mydas) from Arvoredo Island, a foraging ground in southern Brazil, and identified eight haplotypes. Of these, CM-A8 (64%) and CM-A5 (22%) were dominant, the remainder presenting low frequencies (&lt; 5%). Haplotype (h) and nucleotide ( π) diversities were 0.5570 ± 0.0697 and 0.0021 ± 0.0016, respectively. Exact tests of differentiation and AMOVA ΦST pairwise values between the study area and eight other Atlantic foraging grounds revealed significant differences in most areas, except Ubatuba and Rocas/Noronha, in Brazil (p &gt; 0.05). Mixed Stock Analysis, incorporating eleven Atlantic and one Mediterranean rookery as possible sources of individuals, indicated Ascension and Aves islands...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Foraging grounds; Genetic diversity; Green turtle; MtDNA haplotypes; Natal origins.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300027

First report of White spot syndrome virus in farmed and wild penaeid shrimp from Lagoa dos Patos estuary, southern Brazil
Provedor de dados: 58
Autores: Cavalli,Lissandra Souto; Romano,Luis Alberto; Marins,Luis Fernando; Abreu,Paulo César.
In this study, we detected White spot syndrome virus (WSSV) in wild Farfantepenaeus paulensis collected in the Lagoa dos Patos estuary and cultivated Litopenaeus vannamei. This is the first report of WSSV in F. paulensis from Lagoa dos Patos and farmed L. vannamei shrimps in Rio Grande do Sul.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: WSSV; Viral diseases; Farfantepenaeus paulensis; Litopenaeus vannamei; Epizootiology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300042