Consulta: creatorFacets:"Mattar,R."
Registros recuperados: 2
Data/hora: 11/06/2024 18:38:29

Infrequent p53 gene alterations in ulcerative colitis
Provedor de dados: 56
Autores: Mattar,R.; Alexandrino,A.M.; Laudanna,A.A.
The purpose of this study was to determine whether point mutations and loss of the p53 gene take place in ulcerative colitis which is histologically negative for dysplasia. DNA was extracted from 13 frozen rectal or colon biopsies and blood samples. Ulcerative colitis was classified histologically as active (10 cases) and inactive (3 cases). Exons 5-8 were amplified by PCR, treated with exonuclease and shrimp alkaline phosphatase and sequenced by the dideoxy chain termination method with the Sequenase Version 2.0 DNA sequencing kit. PCR products of intron 6 and exon 4 were digested with MspI and AccII, respectively, for RFLP analysis. No p53 gene mutation was detected in these cases. The number of informative patients for loss of heterozygosity (LOH) at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P53 gene; Ulcerative colitis; LOH; Mutation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900005

Pamidronate for the treatment of osteoporosis secondary to chronic cholestatic liver disease in Wistar rats
Provedor de dados: 56
Autores: Pereira,F.A.; Mattar,R.; Facincani,I.; Defino,H.L.A.; Ramalho,L.N.Z.; Jorgetti,V.; Volpon,J.B.; Paula,F.J.A. de.
Osteoporosis is a major complication of chronic cholestatic liver disease (CCLD). We evaluated the efficacy of using disodium pamidronate (1.0 mg/kg body weight) for the prevention (Pr) or treatment (Tr) of cholestasis-induced osteoporosis in male Wistar rats: sham-operated (Sham = 12); bile duct-ligated (Bi = 15); bile duct-ligated animals previously treated with pamidronate before and 1 month after surgery (Pr = 9); bile duct-ligated animals treated with pamidronate 1 month after surgery (Tr = 9). Rats were sacrificed 8 weeks after surgery. Immunohistochemical expression of IGF-I and GH receptor was determined in the proximal growth plate cartilage of the left tibia. Histomorphometric analysis was performed in the right tibia and the right femur was used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic osteodystrophy; Osteoporosis pathogenesis; Growth factors; Biomechanics; Animal models; Pamidronate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200021