Consulta: creatorFacets:"Meireles,M.C.A."
Registros recuperados: 13
Data/hora: 16/06/2024 11:23:47

Chemical and cytotoxic analyses of three varieties of Brazilian propolis (green propolis, jataí propolis and brown propolis) and its anti-Sporothrix brasiliensis in vitro activity
Provedor de dados: 45
Autores: Peter,C.M.; Waller,S.B.; Picoli,T.; Osório,L.G.; Zani,J.L.; Meireles,M.C.A.; Faria,R.O.; Mello,J.R.B.; Hubner,S.O.; Lima,M.; Fischer,G.
ABSTRACT In this study, we described the antifungal activity of three Brazilian propolis extracts: brown, green and from jataí bees against Sporothrix brasiliensis. The extracts were obtained from ethanolic extraction and their chemical composition was determined by high-performance liquid chromatography coupled to mass spectrometry. The cellular toxicity was measured in MDBK (Madin-Darby Bovine Kidney) cells and quantified by the MTT assay (3- (4,5 dimethylthiazol-2yl -2,5-diphenyl-2H bromine tetrazolato). For antifungal activity, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined by broth microdilution. The results showed that cell toxicity was not observed at lower concentrations (0.097 to 0.39μg/ml)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zoonosis; Itraconazole; Antifungal resistance; Therapeutic alternative.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300819

Dermatite multifatorial em um canino
Provedor de dados: 45
Autores: Matos,C.B.; Madrid,I.M.; Santin,R.; Azambuja,R.H.; Schuch,I.; Meireles,M.C.A.; Cleff,M.B.
Relatou-se um caso clínico de dermatite multifatorial em cão, envolvendo Demodex canis, Sporothrix schenckii e bactérias dos gêneros Staphylococcus e Streptococcus. O animal, macho, da raça Pit Bull, com um ano e três meses de idade, apresentou lesões úmidas, hiperêmicas, descamativas e ulcerativas nas regiões do pescoço e do tórax. Exames laboratoriais confirmaram o diagnóstico de infecção concomitante por fungo, bactéria e parasito. A terapia antimicrobiana com moxidectina, itraconazol e amoxicilina foi efetiva, com remissão dos sinais clínicos após 30 dias de tratamento. Este relato salienta a importância do diagnóstico de certeza, que foi determinante para estabelecimento da terapêutica adequada e recuperação do animal.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cão; Demodicose; Esporotricose; Piodermite.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600011

Eficácia da clorexidina-cetrimida na desinfecção ambiental contra Aspergillus spp.
Provedor de dados: 45
Autores: Xavier,M.O.; Meinerz,A.R.M.; Cleff,M.B.; Osório,L.G.; Schuch,L.F.D.; Nobre,M.O.; Silva Filho,R.P.; Meireles,M.C.A.
Avaliou-se a ação in loco da clorexidina-cetrimida no controle de Aspergillus spp., considerando-se a influência de fatores climáticos e populacionais, em um centro de recuperação de animais marinhos. Durante dois anos, realizaram-se colheitas de amostras de ar por meio da técnica de sedimentação, com implantação no segundo ano de um programa de desinfecção com clorexidina-cetrimida. Os resultados do isolamento fúngico nos dois anos foram comparados estatisticamente pelo programa Epinfo 8.0. Demonstrou-se que o isolamento de Aspergillus spp. não apresentou relação significativa com fatores climáticos e populacionais e que a diminuição da concentração de conídios de Aspergillus spp. do ambiente ocorreu devido ao programa eficaz de desinfecção com a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus spp.; Clorexidina-cetrimida; Desinfetante.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400015

Fungi in the respiratory tract of horses with recurrent airway obstruction
Provedor de dados: 45
Autores: Xavier,M.O.; Nogueira,C.E.W.; Meirelles,M.; Fernandes,W.R.; Andreolla,H.; Severo,L.C.; Pasqualotto,A.C.; Meireles,M.C.A.
This study aims to compare the concentration of viable fungi, especially those of the genus Aspergillus in the respiratory tract of stabled horses with and without Recurrent Airway Obstruction (RAO). Thirty two housed horses from four equestrian centers in Brazil were included in the study. These animals were submitted to clinical examination and to a respiratory sample collection. They were categorized into two groups: healthy and RAO-affected horses. Samples obtained by tracheobronchial washes were evaluated for fungal microscopy, quantitative culture and Aspergillus spp. quantification. Eighteen healthy and 14 RAO-affected horses were studied. Fungi were more frequently recovered in the RAO group, in comparison to controls, for both fungal microscopy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus; Tracheobronchial washes; Respiratory allergy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501457

Atividade inibitória do óleo essencial de orégano em fungos de importância médica e veterinária
Provedor de dados: 45
Autores: Cleff,M. B.; Meinerz,A.R.; Faria,R.O.; Xavier,M.O.; Santin,R.; Nascente,P.S.; Rodrigues,M.R.; Meireles,M.C.A.
Tipo: Info:eu-repo/semantics/article
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000500040

Study of bacteria isolated from the foot pad of Spheniscus magellanicus with and without bumblefoot
Provedor de dados: 45
Autores: Osório,L.G.; Xavier,M.O.; Ladeira,S.R.L.; Silva Filho,R.P.; Faria,R.O.; Vargas,G.D'Á.; Cabana,A.L.; Mello,J.R.B.; Meireles,M.C.A.
The bumblefoot or pododermatitis is among the diseases with the highest morbidity in Magellanic penguins, sometimes evolving to septicemia and death. Therefore, this study aimed to relate the main species involved in the disorder, as well as the in vitro susceptibility profile of the microorganisms against routine antimicrobial usage in Veterinary Medicine. During two years in vivo material was harvested from 200 footpads (n=100 animals) for microbiological analysis and in vitro susceptibility tests against the Antibiotic enrofloxacin, streptomycin, penicillin and cephalosporin. Bacteria have been identified both as part of permanent and transient microbiota, also being associated to 100% of the pododermatitis cases. The most prevalent genus were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pododermatitis; Magellanic penguins; Bacterial susceptibility.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100008

Blood parameters and measurements of weight in the rehabilitation of Magellanic penguins (Spheniscus magellanicus, Foster 1781)
Provedor de dados: 45
Autores: Martins,A.M.; Silva Filho,R.P.; Xavier,M.O.; Meireles,M.C.A.; Robaldo,R.B.
During the austral winter the appearance of the Magellanic penguin (Spheniscus magellanicus) is common on the southern coast of Brazil. The oil pollution constitutes a major cause of death of these birds. The monitoring of the weight and blood variables is important to make decisions during the rehabilitation and release of these animals. Thus, the aim of this study was to evaluate the relationship between the penguins' survival during rehabilitation and the values of hematocrit (Hct), total plasma protein (TPP) and body weight (BW) of the birds received at Centro de Recuperação de Animais Marinhos (Recovery Centre for Sea Animals) between 2006 and 2009. The analysis took place weekly, and penguins were classified according to the outcome of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sphenisciformes; Oiled; Hematocrit; Total plasma protein; Weight.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000100125

Atividade in vitro do óleo essencial de Origanum vulgare L. em isolados clínicos de Aspergillus spp.
Provedor de dados: 45
Autores: Osório,L.G.; Silva,A.L.; Fonseca,A.O.S.; Dias,T.P.; Martins,O.A.; Faria,R.O.; Meireles,M.C.A.; Cleff,M.B.; Freitag,R.; Mello,J.R.B.
RESUMO Aspergillus fumigatus, A. flavus e A. niger são os mais importantes agentes etiológicos da aspergilose, relevante micose aviária, com tratamento ineficaz e altas taxas de mortalidade. Em vista da importância da aspergilose, da necessidade de prospectar novos fármacos e do potencial terapêutico do óleo essencial de Origanum vulgare L. (OEO), o orégano, objetivou-se avaliar a sensibilidade in vitro de isolados clínicos de Aspergillus spp. em relação ao OEO. O óleo foi obtido por hidrodestilação em Clevenger, e a análise química realizada por cromatografia de massa (GC/MS). Observaram-se 15 diferentes compostos ativos, sendo 4-terpineol, hidrato de sabinene e timol os majoritários. Nos testes de microdiluição em caldo (Reference..., 2008), todos os...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orégano; Atividade anti-Aspergillus; Lamiaceae; Antifungigrama.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100204

Esporotricose experimental sistêmica em ratos Wistar: avaliação hematológica e perfil hepático
Provedor de dados: 45
Autores: Meinerz,A.R.M.; Antunes,T.A.; Silva,F.V.; Xavier,M.O.; Cleff,M.B.; Meireles,M.C.A.
Alanine aminotransferase (ALT) and alkaline phosphatase (AP) hepatic enzymes, hematological values, and total plasmatic proteins (TPP) of 12 Wistar rats with systemic experimental sporotrichosis were evaluated. Inoculation was performed at 2x10³ cells of S. schenckii/ml. The values ranged from 35 to 48UI/l for ALT and 67 to 251.3UI/l for AP. Fifty percent of the AP values were above the physiological limits. TPP was elevated in 66.7% (8/12) of the rats with values varying from 7.9 to 8.5g%. In 75% (9/12) of the animals, it was observed that hemoglobin and red blood cells count were below the physiologic limits, and 8.3% (1/12) of the animals showed anemia masked by dehydration. At the necropsy, it was observed local and/or disseminated nodular lesions. At...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rato; Esporotricose experimental; Sporotrix schenckii; Enzimas hepáticas.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400038

Atividade antifúngica do óleo essencial de Origanum vulgare frente a Malassezia pachydermatis
Provedor de dados: 45
Autores: Santin,R.; Giordani,C.; Madrid,I.M.; Matos,C.B.; Freitag,R.A.; Meireles,M.C.A.; Cleff,M.B.; Mello,J.R.B.
Objetivou-se com este estudo avaliar a atividade antifúngica in vitro do óleo essencial de Origanum vulgare frente a isolados clínicos de Malassezia pachydermatis. As folhas secas de O. vulgare foram adquiridas de distribuidor comercial com certificado de qualidade e origem e encaminhadas para extração do óleo essencial e cromatografia. Para realização do teste in vitro, foi utilizada a técnica de microdiluição em caldo (CLSI M27A3) com modificações para fitofármacos e M. pachydermatis. O óleo essencial de orégano foi testado nas concentrações de 28 a 0,87mg/mL diluído em caldo Sabouraud com 1% de tween 80. Todos os isolados foram testados em duplicata. Na análise cromatográfica do óleo essencial, foram identificados 12 compostos, sendo timol, a-terpineno...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cão; Extratos vegetais; Leveduras; Orégano; Suscetibilidade.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200007

Effects of essential oils of Rosmarinus officinalis Linn. and Origanum vulgare Linn. from different origins on Sporothrix brasiliensis and Sporothrix schenckii complex
Provedor de dados: 45
Autores: Waller,S.B.; Madrid,I.M.; Cleff,M.B.; Santin,R.; Freitag,R.A.; Meireles,M.C.A.; Mello,J.R.B.
ABSTRACT Rosmarinus officinalis L. (rosemary) and Origanum vulgare L. (oregano) are known to have antimicrobial properties, but studies on sporotrichosis are scarce. This study aimed to evaluate the anti-Sporothrix spp. activity of essential oils from commercial products and oils extracted from aerial parts of these plants and analyze their chemical constituents. S. schenckii complex and S. brasiliensis (n: 25) isolated from humans, cats, dogs, and environmental soil were tested through M27-A3 guidelines of CLSI with modification for phytotherapics. The essential oils of R. officinalis L. were similar for MIC50 and MFC50 ≤2.25mg/mL for extracted oil; and 4.5mg/mL and 9mg/mL, respectively, for commercial oil. Both products showed MIC90 of 18mg/mL and MFC90...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sporotrichosis; Antifungal resistance; Oregano; Rosemary; Lamiaceae.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000400991

Atividade in vitro do óleo essencial de Origanum vulgare frente à Sporothrix Schenckii
Provedor de dados: 45
Autores: Cleff,M.B.; Meinerz,A.R.M.; Schuch,L.F.D.; Rodrigues,M.R.A.; Meireles,M.C.A.; Mello,J.R.B.
In vitro activity of the essential oil Origanum vulgare against Sporothrix schenckii was determined by the MIC (Minimum Inhibitory Concentration). For this, seven samples of S. schenckii were studied, two isolated from two cases of human sporotrichosis and five isolated from cats. Analysis of the essential oil was carried out in a gas chromatograph (GC/FID) for the identification and quantification of thymol and carvacrol (antifungal agents). MIC was obtained based on the microdilution method according to the adapted document NCCLS-M 27A2 for fitopharmacy. All the isolates presented sensibility to the essential oil. S. schenckii was inhibited in a concentration of 0.25% (250m l/ml). Chromatographic analysis showed that thymol concentration was bigger than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sporothrix schenckii; Origanum vulgare; Orégano; Tratamento.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200039

In vitro activity of disinfectants against Aspergillus spp
Provedor de dados: 58
Autores: Mattei,A.S.; Madrid,I.M.; Santin,R.; Schuch,L.F.D.; Meireles,M.C.A.
Fungi of the Aspergillus genus are widespread and contaminate the environment. Thousands of conidia are released from each phialide and dispersed in the air every day. These fungi are considered important mycose-causing agents in hospitals. Due to this, research to determine prevalent fungi from the Aspergillus genus in hospital environments, and an adequate disinfection program in these areas is are needed. This study evaluated the susceptibility of Aspergillus spp. isolated from a veterinary environment against four disinfectants. Successive dilutions of disinfectants (log2) were used according to CLSI M38-A2 microdilution technique adapted to chemical agents against 18 isolates of this genus. After 72 hours of incubation, the Minimum Inhibiting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammonium quaternary; Chlorexidine; Chlorophenol derivative; Filamentous fungi; Sodium hypochlorite.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200024