Consulta: creatorFacets:"Miglino,M.A."
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Data/hora: 10/06/2024 17:30:15

Ossificação endocondral em embriões e fetos de bovinos
Provedor de dados: 45
Autores: Trujillo,H.A.G.; Alberto,M.L.V.; Braga,M.B.P.; Will,S.E.A.L.; Salvadori,M.L.B.; Ambrósio,C.E.; Miglino,M.A.
Estudou-se a ossificação endocontral de 18 embriões e 12 fetos de até três meses de gestação, os quais foram coletados de úteros gestantes em frigoríficos e abatedouros. Os úteros foram dissecados e, em seguida, realizou-se uma incisão dorsal até o cérvix para avaliações macroscópicas dos embriões e fetos. Para o estudo microscópico foram realizadas técnicas de inclusão, seguidas de marcação dos depósitos de cálcio e fósforo, responsável pela ossificação dos moldes de cartilagem. Foram identificados hipertrofia da cartilagem e morte dos condrócitos e aumento da área de depósito de cálcio e fósforo, por volta da 10ª semana gestacional (74 dias). Durante a 11ª semana de gestação (81 dias), os grupamentos de carbonato de cálcio e fósforo espalharam-se por...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Osteogêneses; Ossificação endocondral; Cálcio; Fósforo.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400002

Concentration of collagen fibers in the musculature of broiler chickens fed with cupuacu seed by-product
Provedor de dados: 45
Autores: Silveira,T.B.; Souza,S.F.; Medeiros,L.S.; Freitas,H.J.; Gracci,R.E.; Miglino,M.A.; Carvalho,Y.K.
ABSTRACT In this essay, we quantify the concentration of collagen fibers in broiler chickens exposed to increasing concentrations of cupuacu seed by-product. Collection of material was carried out in five chickens per treatment at 70 days old in the groups: control, 5% and 10% inclusion of cupuacu seed by-product. Fragments of Thoracic Pectoralis (PT) and Iliotibial lateralis (ITL) muscles were prepared for light and electronic microscopy. The amount of collagen fibers in the muscle groups was 1.08±0.61% in the PTC group; 6.24±2.58% in PT5% and 7.30±2.75% in PT10%. In the Iliotibial Lateralis groups, the results were 6.96±3.14% in the ITLC; 7.43±4.22% in the ITL5% and 8.66±2.35% in ITL10%. The amount of collagen fibers in the ITL5% and ITL10% groups showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler chickens; Collagen; Theobroma grandiflorum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300791

Bone marrow mononuclear cells versus mesenchymal stem cells from adipose tissue on bone healing in an Old World primate: can this be extrapolated to humans?
Provedor de dados: 45
Autores: Branco,E.; Miranda,C.M.F.C.; Lima,A.R.; Silva,K.S.M.; Cabral,R.M.; Miranda,M.S.; Ohashi,O.M.; Oliveira,E.H.C.; Silva,L.S.C.; Freitas,D.M.M.; Miglino,M.A.
ABSTRACT In veterinary medicine, the cell therapy is still unexplored and there are many unanswered questions that researchers tend to extrapolate to humans in an attempt to treat certain injuries. Investigating this subject in nonhuman primates turns out to be an unparalleled opportunity to better understand the dynamics of stem cells against some diseases. Thus, we aimed to compare the efficiency of bone marrow mononuclear cells (BMMCs) and mesenchymal stem cells (MSCs) from adipose tissue of Chlorocebus aethiops in induced bone injury. Ten animals were used, male adults subjected, to bone injury the iliac crests. The MSCs were isolated by and cultured. In an autologous manner, the BMMCs were infused in the right iliac crest, and MSCs from adipose tissue...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell therapy; Chlorocebus aethiops; Bone injury; Mesenchymal stem cells from adipose tissue.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300917

Tongue papillae morphology of brown-throated sloth Bradypus variegatus (SCHINZ, 1825)
Provedor de dados: 45
Autores: Martins,D.M.; Pinheiro,L.L.; Ferreira,V.C.; Costa,A.M.; Lima,A.R.; Ricci,R.E.G.; Miglino,M.A.; Branco,E.
The Bradypusvariegatus inhabits the forests of South America and feeds from leaves, branches and sprouts from different plants. Due to its diet and the lack of literature on the morphological aspect of Xenarthras, five Bradypusvariegatus tongues from animals which died from natural causes were evaluated, and they came from Pará State Museum Emílio Goeldi and were donated to the Laboratory of Animal Morphological Research (LaPMA) from UFRA, for revealing the different types of papillae and epithelial-connective tissue. Macroscopically, the tongues presented elongated shape, rounded apex, body, root, median sulcus in the root's apex, and two vallate papillae. The mucous membrane of the tongue revealed a keratinized stratified pavement epithelium, while the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphology; Lingual papillae; Bradypus variegatus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501479

Bovine conceptus of Bos indicus produced by somatic cell nuclear transfer and parthenogenesis present morphological variations since the blastocyst stage
Provedor de dados: 45
Autores: Oliveira,F.D.; Sangalli,J.R.; Meirelles,F.V.; Perecin,F.; Silva Filho,P.P.C.; Watanabe,Y.F.; Miglino,M.A.; Assis Neto,A.C.
In cattle, embryo development is characterized by the appearance of two distinct cell layers, the trophectoderm and the inner cell mass. The latter will undergo differentiation to form the embryonic disc consisting of the epiblast and hypoblast. The aim of this study was to ultrastructurally characterize the bovine embryo from different in vitro production techniques, with emphasis on trophectoderm and inner cell mass cells. Bovine embryos on day 7 (conception = D1) of pregnancy, derived via in vitro production techniques, were fixed for light and transmission electron microscopy processing. Results suggested that embryos produced by nuclear transfer of somatic cells and parthenogenesis showed significant changes in macroscopic and microscopic structure....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Blastocyst; In vitro production; Ultrastructure.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601483

Efficacy and limitations of different approaches to anticipate the diagnosis of pregnancy in cattle
Provedor de dados: 45
Autores: Palhão,M.P.; Guimarães,C.R.B.; Lima,J.F.J.F.M.; Mendonça,M.R.; Fernandes,C.A.C.; Neves,J.P.; Garcia,J.A.D.; Gioso,M.M.; Miglino,M.A.; Viana,J.H.M.
ABSTRACT The study evaluated sonographic and serologic exams performed for early (20 to 30d) diagnosis of pregnancy. One hundred-twenty (n= 120) bovine recipients were synchronized (estrous=D0) and timed embryo transferred (TET, D7) with fresh in vitro produced embryos. In the first trial (n= 46), diagnosis of pregnancy was performed on day 20 (D20) by detecting CL blood flow (BF) and by Pregnancy-Associated Glycoproteins (PAGs) serology. In the second trial (n= 30), pregnancy diagnosis was performed on D25 by ultrasound visualization of uterine contents and by PAGs serology. In the last trial, PAG’s serology was performed on D30. Ultrasonographic detection of the uterine contents and embryo viability performed on D30 (DG30) was considered the gold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Color doppler; PAGs; Timed embryo transfer.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601909

Decellularization of placentas: establishing a protocol
Provedor de dados: 56
Autores: Leonel,L.C.P.C.; Miranda,C.M.F.C.; Coelho,T.M.; Ferreira,G.A.S.; Caãada,R.R.; Miglino,M.A.; Lobo,S.E.
Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and have not been established for canine placentas yet. This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas. Canine placentas were subjected to ten preliminary tests to analyze the efficacy of parameters such as the type of detergents, freezing temperatures and perfusion. Two protocols were chosen for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Placenta; Decellularization; Acellular biomaterial.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100601