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AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
Provedor de dados: 1
Autores: Andrade, Felipe Aranha de; Gonçalves, Leandro Simões Azeredo; Miglioranza, Édison; Ruas, Claudete de Fátima; Ruas, Paulo Mauricio; Takahashi, Lúcia Sadayo Assari.
The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genética e Melhoramento Phaseolus vulgaris L.; Molecular markers; Amplified Fragment Length Polymorphism; Gene bank; Genetic diversity Recursos Genéticos Phaseolus vulgaris L.; Molecular markers; Amplified fragment length polymorphism; Gene bank.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577