Consulta: creatorFacets:"Milling,Annett"
Registros recuperados: 1
Data/hora: 04/06/2024 15:53:35

An optmized protocol for simultaneous extraction of DNA and RNA from soils
Provedor de dados: 58
Autores: Costa,Rodrigo; Gomes,Newton C.M.; Milling,Annett; Smalla,Kornelia.
In this work we report an optimized protocol for simultaneous extraction of DNA and RNA from soil matrices. Treatment of soil matrices with ethanol followed by bead-beating worked as a successful strategy to lyse the cells without considerable degradation of nucleic acids, resulting in DNA and RNA of good yield and integrity. The reverse transcribed RNA could be amplified with primers targeting a glutamine synthetase (glnA) gene fragment. From both DNA and cDNA, 16S rDNA fragments were amplified and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). The method was applied to soil and rhizosphere (strawberry and oilseed rape) samples. Two other protocols for the extraction of nucleic acids from soil were applied to the same set of samples in order...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA extraction; Nucleic acids; Rhizosphere; Microbial communities.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200011