Consulta: creatorFacets:"Miranda,Vitor Fernandes Oliveira de"
Registros recuperados: 3
Data/hora: 03/06/2024 02:48:11

Aspectos morfo-anatômicos de três espécies de Drosera, durante o desenvolvimento pós-seminal
Provedor de dados: Acta Botanica Brasilica
Autores: Miranda,Vitor Fernandes Oliveira de; Sajo,Maria das Graças.
Durante o processo de germinação, as sementes das três espécies (Drosera spathulata Labill., D. intermedia Dreves &amp;amp; Hayne e D. burmanni Vahl) rompem-se por meio de um opérculo e as plántulas desenvolvem pêlos unicelulares ancoradouros antes do estabelecimento da raiz primária. A porção apical dos cotilédones permanece no interior dos envoltórios das sementes e possui função haustorial. Diferentes tricomas são constatados nas folhas e cotilédones das plántulas, inclusive na superfície das emergências. Alguns aspectos morfo-anatômicos das folhas também são apresentados.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Desenvolvimento pós-seminal; Drosera; Anatomia foliar.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062000000200007

Plant or fungal sequences? An alternative optimized PCR protocol to avoid ITS (nrDNA) misamplification
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Miranda,Vitor Fernandes Oliveira de; Martins,Vanderlei Geraldo; Furlan,Antonio; Bacci Jr.,Maurício.
The nuclear ribosomal DNA internal transcribed spacers (ITS1 and ITS2) from leaves of Drosera (Droseraceae) were amplified using "universal" primers. The analysis of the products demonstrated most samples were a molecular mixture as a result of unsuccessful and non-specific amplifications. Among the obtained sequences, two were from Basidiomycota fungi. Homologous sequences of Basidiomycota were obtained from GenBank database and added to a data set with sequences from Drosera leaves. Parsimony analysis demonstrated that one sequence was amplified from an Ustilaginomycetes fungus, and another from a Heterobasidiomycetes. Possibly these fungi were associated to leaves of Drosera, and not because of samples contamination. In order to provide optimization and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase; DNA; Drosera; Fungi; Phylogeny.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100018

16s-23S rDNA: polymorphisms and their use for detection and identification of Xylella fastidiosa strains
Provedor de dados: Brazilian Journal of Microbiology
Autores: Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui.
Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033