Consulta: creatorFacets:"Miyaki,Cristina Y."
Registros recuperados: 4
Data/hora: 29/05/2024 06:04:43

Comparative analysis of microsatellite variability in five macaw species (Psittaciformes, Psittacidae): application for conservation
Provedor de dados: Genetics and Molecular Biology
Autores: Presti,Flavia T.; Oliveira-Marques,Adriana R.; Caparroz,Renato; Biondo,Cibele; Miyaki,Cristina Y.
Cross-amplification was tested and variability in microsatellite primers (designed for Neotropical parrots) compared, in five macaw species, viz., three endangered blue macaws (Cyanopsitta spixii [extinct in the wild], Anodorhynchus leari [endangered] and Anodorhynchus hyacinthinus [vulnerable]), and two unthreatened red macaws (Ara chloropterus and Ara macao). Among the primers tested, 84.6% successfully amplified products in C. spixii, 83.3% in A. leari, 76.4% in A. hyacinthinus, 78.6% in A. chloropterus and 71.4% in A. macao. The mean expected heterozygosity estimated for each species, and based on loci analyzed in all the five, ranged from 0.33 (A. hyacinthinus) to 0.85 (A. macao). As expected, the results revealed lower levels of genetic variability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Cross-amplification; Macaw; Microsatellite; Psittaciformes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200030

Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus)
Provedor de dados: Genetics and Molecular Biology
Autores: Presti,Flavia T.; Meyer,Janaína; Antas,Paulo T.Z.; Guedes,Neiva M.R.; Miyaki,Cristina Y.
Molted feather sampling is a useful tool for genetic analyses of endangered species, but it is often very laborious due to the low quality and quantity of the DNA obtained. In the present study we show the parts of feathers that resulted in better yield of DNA. In descending order these were: blood clot outside the umbilicus, umbilicus (without blood clot), tip, inner membrane, and small calamus. Compared to DNA extracted from blood samples, DNA extracted from feathers produced microsatellite alleles of poorer quality and had to be processed immediately after extraction. As expected due to the level of DNA degradation, molecular sexing protocols that result in shorter PCR products were more efficient.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molted feathers; Blood sample; DNA; PCR product; Parrot.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100019

Genetic evaluation of the mating system in the blue-and-yellow macaw (Ara ararauna, Aves, Psittacidae) by DNA fingerprinting
Provedor de dados: Genetics and Molecular Biology
Autores: Caparroz,Renato; Miyaki,Cristina Y.; Baker,Allan J.
More than 90% of birds are socially monogamous, although genetic studies indicate that many are often not sexually monogamous. In the present study, DNA fingerprinting was used to estimate the genetic relationships between nestlings belonging to the same broods to evaluate the mating system in the socially monogamous macaw, Ara ararauna. We found that in 10 of 11 broods investigated, the nestlings showed genetic similarity levels congruent with values expected among full-sibs, suggesting that they shared the same parents. However, in one brood, the low genetic similarity observed between nestlings could be a result of intraspecific brood parasitism, intraspecific nest competition or extra-pair paternity. These results, along with available behavioral and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ara ararauna; Breeding behavior; DNA fingerprinting; Monogamy; Psittacidae.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100027

Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)
Provedor de dados: Genetics and Molecular Biology
Autores: Faria,Patrícia J.; Miyaki,Cristina Y.
The selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus), 42 blue-and-yellow macaws (Ara ararauna), 23 red-and-green macaws (Ara chloroptera), 19 red-spectacled amazons (Amazona pretrei); and 18 red-tailed amazons (Amazona brasiliensis). We tested 21 clones from a genomic library of golden conure (Guarouba guarouba) minisatellites and 12 pairs of microsatellite primers developed for the domestic chicken (Gallus gallus) and A. hyacinthinus. We also tested seven...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Minisatellites; Molecular markers; Parrots; Population studies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200007