Consulta: creatorFacets:"Monteiro,Cristina Leise Bastos"
Registros recuperados: 4
Data/hora: 09/06/2024 08:33:46

Enrichment methodology to increase the positivity of cultures from body fluids
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Daur,Alessandra Valle; Klimak Jr.,Francisco; Cogo,Laura Lúcia; Botão,Gislene Diógenes; Monteiro,Cristina Leise Bastos; Dalla Costa,Libera Maria.
Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert® blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Body fluids; Etiological agents; Enrichment.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600002

Characterization of virulence genes cagA and vacA in Helicobacter Pylori and their prevalence in gastrointestinal disorders
Provedor de dados: Brazilian Journal of Microbiology
Autores: Cogo,Laura Lúcia; Monteiro,Cristina Leise Bastos; Nogueira,Keite da Silva; Palmeiro,Jussara Kasuko; Ribeiro,Marcelo Lima; Camargo,Eloá Ramalho de; Neves,Daniel Locatelli; Nascimento,Aguinaldo José do; Costa,Libera Maria Dalla.
Prevalence of H. pylori infection was determined using cultures of gastric biopsy samples of patients attended at the academic hospital of the Federal University of Paraná, Curitiba, Paraná, Brazil. Molecular methods were used to characterize the cagA and vacA genes from bacterial isolates associated with different diseases presented by patients. Out of a total of 81, forty-two gastric biopsy samples tested were positive for H. pylori, with a prevalence of 51.9%. No significant difference was found with regard to the gender (p=0.793) and age (p=0.183) of the patients. Genotype s1m1 vacA gene was found in 67% of the cases of peptic ulcer investigated (p=1.0), despite the limited number of patients with this disease (n=3). A correlation between the presence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Virulence genes; CagA; VacA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400007

Anti-Helicobacter pylori activity of plant extracts traditionally used for the treatment of gastrointestinal disorders
Provedor de dados: Brazilian Journal of Microbiology
Autores: Cogo,Laura Lúcia; Monteiro,Cristina Leise Bastos; Miguel,Marilis Dallarmi; Miguel,Obdulio Gomes; Cunico,Miriam Machado; Ribeiro,Marcelo Lima; Camargo,Eloá Ramalho de; Kussen,Gislene Maria Botão; Nogueira,Keite da Silva; Costa,Libera Maria Dalla.
The antibacterial activity of plant extracts obtained from Bixa orellana L., Chamomilla recutita L., Ilex paraguariensis A. St.-Hil., Malva sylvestris L., Plantago major L. and Rheum rhaponticum L. has been evaluated against two reference strains and eleven clinical isolates of Helicobacter pylori. All the plant species chosen are used in popular Brazilian cuisine and folk medicine in the treatment of gastrointestinal disorders. Initial screening was made by the disk diffusion test and then minimum inhibitory concentration was determined by the agar dilution method. The results presented in this work demonstrated that among the plant preparations analyzed, B. orellana L., C. recutita L., I. paraguariensis A. St.-Hil. and M. sylvestris L. were capable of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Antibacterial activity; Plant extracts.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200007

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Benetti,Thalyta Marina; Monteiro,Cristina Leise Bastos; Beux,Márcia Regina; Abrahão,Wanda Moscalewski.
The aim of this study was to determine the prevalence of Listeria sp. in refrigerated sausages, and to compare the performance of the selective plating media employed in the ISO 11290-1 method (PALCAM and Oxford agars) with chromogenic agars (Chromogenic Listeria agars CM 1080 (OCLA) and CM 1084). The prevalence of Listeria sp. detected was 52.9%, comprising 13.7% L. monocytogenes strains. The efficacy of the four agars for the isolation of L. monocytogenes proved to be satisfactory. Despite differences in composition of the chromogenic media assessed, these disparities did not affect concordance among results. However, PALCAM agar was shown to suppress other microorganisms more effectively, being more applicable for detecting Listeria strains present in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria sp./detection; Listeria sp./meat products; Meat products/qualitative analysis; Refrigerated sausages/qualitative analysis; PALCAM Agar; Oxford Agar; Chromogenic Agar.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400011