Consulta: creatorFacets:"Moon,D.H."
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Data/hora: 13/06/2024 07:24:56

An alternative method for Staphylococcus aureus DNA isolation
Provedor de dados: 45
Autores: Chapaval,L.; Moon,D.H.; Gomes,J.E.; Duarte,F.R.; Tsai,S.M.
This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Genomic DNA; DNA isolation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004