Consulta: creatorFacets:"Morsella,Claudia"
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Data/hora: 13/06/2024 05:50:23

Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR
Provedor de dados: 58
Autores: Gilardoni,Liliana Rosa; Fernández,Bárbara; Morsella,Claudia; Mendez,Laura; Jar,Ana María; Paolicchi,Fernando Alberto; Mundo,Silvia Leonor.
Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samples from 265 lactating Holstein cows from Map infected and uninfected herds in Argentina. IMS-IS1 PCR results were analyzed and compared with those obtained from milk and fecal culture and serum ELISA. The extent of agreement between both tests was determined by the Kappa test. IMS-IS1 PCR showed a detection limit of 101 CFU of Map/mL of milk, when 50:50 mix of monoclonal and polyclonal antibodies were used to coat magnetic beads. All of the 118 samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium avium subsp. paratuberculosis; Immunomagnetic separation; PCR; Cow's milk.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200506

First isolation of Mycobacterium avium subsp paratuberculosis from commercial pasteurized milk in Argentina
Provedor de dados: 58
Autores: Paolicchi,Fernando; Cirone,Karina; Morsella,Claudia; Gioffré,Andrea.
Mycobacterium avium subsp paratuberculosis was isolated from two out of seventy samples (2.86 %) of pasteurized and ultra-pasteurized milk. The isolates were positives to IS900 PCR and showed a C17 RFLP pattern, the most prevalent in Argentina. The present study is the first report of Mycobacterium avium subsp paratuberculosis culture from pasteurized milk in Argentina.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Mycobacterium avium subsp paratuberculosis; Pasteurized milk; PCR IS900.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300028

Bovine response to lipoarabinomannan vaccination and challenge with Mycobacterium paratuberculosis
Provedor de dados: 58
Autores: Jolly,Ana; Morsella,Claudia; Bass,Laura; Fiorentino,María Andrea; Paolicchi,Fernando Alberto; Mundo,Silvia Leonor.
This study aimed to evaluate the immune response in bovines following immunization with a mycobaterial Lipoarabinomannan extract (LAMe) and the effect of Map challenge. LAMe vaccine induced specific antibody levels that diminished after the challenge and affected Map excretion at least for 100 days thereafter.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Paratuberculosis; Bovine; LAM; Humoral immune response; Experimental challenge.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200029

Molecular typing of Argentinian Mycobacterium avium subsp. paratuberculosis isolates by multiple-locus variable number-tandem repeat analysis
Provedor de dados: 58
Autores: Gioffré,Andrea; Correa Muñoz,Magnolia; Alvarado Pinedo,María F.; Vaca,Roberto; Morsella,Claudia; Fiorentino,María Andrea; Paolicchi,Fernando; Ruybal,Paula; Zumárraga,Martín; Travería,Gabriel E.; Romano,María Isabel.
Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS900-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Molecular typing; MIRU-VNTR; MLVA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200557