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Data/hora: 02/06/2024 12:58:56

Impairments of mecA gene detection in bovine Staphylococcus spp.
Provedor de dados: Brazilian Journal of Microbiology
Autores: Melo,Dayanne Araújo de; Coelho,Irene da Silva; Motta,Cássia Couto da; Rojas,Anna Carolina Coelho Marín; Dubenczuk,Felipe Carlos; Coelho,Shana de Mattos de Oliveira; Souza,Miliane Moreira Soares de.
Staphylococcus aureus antimicrobial resistance, especially to beta-lactams, favors treatment failures and its persistence in herd environment. This work aimed to develop a more specific primer for mecA gene detection based on the comparison of the conserved regions from distinct host origins and also investigated the presence of homologue mecA LGA251 in bovine strains. A total of 43 Staphylococcus spp. were included in this study, comprising 38 bovine S. aureus, two human and three equine coagulase-negative staphylococci (CNS). Phenotypical methicillin-resistance detection was performed through oxacillin agar-screening and cefoxitin disk-diffusion test. None isolate tested positive for mecA LGA251 gene. For mecA gene PCR, new primers were designed based on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial resistance; Bovine mastitis; Staphylococccus; MecA gene.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300041

Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Marques,Viviane Figueira; Motta,Cássia Couto da; Soares,Bianca da Silva; Melo,Dayanne Araújo de; Coelho,Shana de Mattos de Oliveira; Coelho,Irene da Silva; Barbosa,Helene Santos; Souza,Miliane Moreira Soares de.
Abstract Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation. Biofilm production in microplates was evaluated considering the intervals determined along the bacterial growth curve. In addition, to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm; Agr types; Antimicrobial resistance; Mastitis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100118