Consulta: creatorFacets:"Mouchel, Olivier"
Registros recuperados: 14
Data/hora: 01/06/2024 17:22:57

Metabarcoding is powerful yet still blind: a comparative analysis of morphological and molecular surveys of seagrass communities
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cowart, Dominique A.; Pinheiro, Miguel; Mouchel, Olivier; Maguer, Marion; Grall, Jacques; Mine, Jacques; Arnaud-haond, Sophie.
In the context of the sixth wave of extinction, reliable surveys of biodiversity are increasingly needed to infer the cause and consequences of species and community declines, identify early warning indicators of tipping points, and provide reliable impact assessments before engaging in activities with potential environmental hazards. DNA metabarcoding has emerged as having potential to provide speedy assessment of community structure from environmental samples. Here we tested the reliability of metabarcoding by comparing morphological and molecular inventories of invertebrate communities associated with sea-grasses through estimates of alpha and beta diversity, as well as the identification of the most abundant taxa. Sediment samples were collected from...
Tipo: Text
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00255/36596/35164.pdf

The Congolobe project, a multidisciplinary study of Congo deep-sea fan lobe complex: Overview of methods, strategies, observations and sampling
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rabouille, C.; Olu, Karine; Baudin, F.; Khripounoff, Alexis; Dennielou, Bernard; Arnaud-haond, Sophie; Babonneau, Nathalie; Bayle, Christophe; Beckler, J.; Bessette, Sandrine; Bombled, B.; Bourgeois, S.; Brandily, Christophe; Caprais, Jean-claude; Cathalot, Cecile; Charlier, K.; Corvaisier, R.; Croguennec, Chantal; Cruaud, Perrine; Decker, Carole; Droz, L.; Gayet, Nicolas; Godfroy, Anne; Hourdez, S.; Le Bruchec, J.; Saout, Johan; Le Saout, Marie-helene; Lesongeur, Francoise; Martinez, P.; Mejanelle, L.; Michalopoulos, P.; Mouchel, Olivier; Noel, Philippe; Pastor, Lucie; Picot, M.; Pignet, Patricia; Pozzato, L.; Pruski, A. M.; Rabiller, Manuella; Raimonet, M.; Ragueneau, O.; Reyss, J. L.; Rodier, Philippe; Ruesch, Blandine; Ruffine, Livio; Savignac, F.; Senyarich, C.; Schnyder, J.; Sen, Arunima; Stetten, E.; Sun, Ming Yi; Taillefert, M.; Teixeira, S.; Tisnerat-laborde, N.; Toffin, Laurent; Tourolle, Julie; Toussaint, F.; Vetion, G.; Jouanneau, J. M.; Bez, M.
The presently active region of the Congo deep-sea fan (around 330 000 km2), called the terminal lobes or lobe complex, covers an area of 2500 km2 at 4700–5100 m water depth and 750–800 km offshore. It is a unique sedimentary area in the world ocean fed by a submarine canyon and a channel-levee system which presently deliver large amounts of organic carbon originating from the Congo River by turbidity currents. This particularity is due to the deep incision of the shelf by the Congo canyon, up to 30 km into the estuary, which funnels the Congo River sediments into the deep-sea. The connection between the river and the canyon is unique for major world rivers.  In 2011, two cruises (WACS leg 2 and Congolobe) were conducted to simultaneously investigate the...
Tipo: Text
Palavras-chave: Chemosynthetic habitats; Congo deep-sea fan; Fine sediment; Sedimentation rate; Seafloor morphology; Turbidite; Vesicomyidae.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00335/44580/44298.pdf

Residual genetic variability in domesticated populations of the Pacific blue shrimp (Litopenaeus stylirostris) of New Caledonia, French Polynesia and Hawaii and some management recommendations
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Goyard, Emmanuel; Arnaud, Sophie; Vonau, Vincent; Bishoff, Vincent; Mouchel, Olivier; Pham, Dominique; Wyban, Jim; Boudry, Pierre.
The Latin American shrimp Litopenaeus stylirostris was introduced in three different Pacific islands (Tahiti, New Caledonia via Tahiti, and Hawaii) and hatchery-propagated for 7-25 generations to develop shrimp farming based on these domesticated stocks. Three microsatellite markers have been used in an attempt to assess the genetic bases of the populations available to start a selective breeding program. The comparison of eight hatchery stocks (five New Caledonian, two Hawaiian and one Tahitian stocks) and one wild Ecuadorian population showed a much lower variability in the domesticated stocks than in the wild population, especially in New Caledonia and Tahiti (2-3.7 vs. 14-27 alleles per locus; 20-60% vs. 90% expected heterozygosity). The Tahitian and...
Tipo: Text
Palavras-chave: Microsatellite marker; Genetic variability; Domestication; Litopenaeus stylirostris; Shrimp.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-596.pdf

Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Becheler, Ronan; Cassone, Anne-laure; Noel, Philippe; Mouchel, Olivier; Morrison, Cheryl L.; Arnaud-haond, Sophie.
Sampling in the deep sea is a technical challenge, which has hindered the acquisition of robust datasets that are necessary to determine the fine-grained biological patterns and processes that may shape genetic diversity. Estimates of the extent of clonality in deep-sea species, despite the importance of clonality in shaping the local dynamics and evolutionary trajectories, have been largely obscured by such limitations. Cold-water coral reefs along European margins are formed mainly by two reef-building species, Lophelia pertusa and Madrepora oculata. Here we present a fine-grained analysis of the genotypic and genetic composition of reefs occurring in the Bay of Biscay, based on an innovative deep-sea sampling protocol. This strategy was designed to be...
Tipo: Text
Palavras-chave: Cold-water coral; Lophelia pertusa; Madrepora oculata; Clonality; Fine-grained spatial genetic structure; Standardized sampling.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00300/41117/40291.pdf

Gene-associated markers provide tools for tackling illegal fishing and false eco-certification
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Nielsen, Einar E.; Cariani, Alessia; Mac Aoidh, Eoin; Maes, Gregory E.; Milano, Ilaria; Ogden, Rob; Taylor, Martin; Hemmer-hansen, Jakob; Babbucci, Massimiliano; Bargelloni, Luca; Bekkevold, Dorte; Diopere, Eveline; Grenfell, Leonie; Helyar, Sarah; Limborg, Morten T.; Martinsohn, Jann T.; Mcewing, Ross; Panitz, Frank; Patarnello, Tomaso; Tinti, Fausto; Van Houdt, Jeroen K. J.; Volckaert, Filip A. M.; Waples, Robin S.; Carvalho, Gary; Albin, Jan Ej; Vieites Baptista, Juan M; Barmintsev, Vladimir; Bautista, Jose M.; Bendixen, Christian; Berge, Jean-pascal; Blohm, Dietmar; Cardazzo, Barbara; Diez, Amalia; Espineira, Montserrat; Geffen, Audrey J.; Gonzalez, Elena; Gonzalez-lavin, Nerea; Guaniero, Ilaria; Jerome, Marc; Kochzius, Marc; Krey, Grigorius; Mouchel, Olivier; Negrisolo, Enrico; Piccinetti, Corrado; Puyet, Antonio; Rastorguev, Sergey; Smith, Jane P; Trentini, Massimo; Verrez-bagnis, Veronique; Volkov, Alexander; Zanzi, Antonella.
Illegal, Unreported and Unregulated fishing has had a major role in the overexploitation of global fish populations. In response, international regulations have been imposed and many fisheries have been 'eco-certified' by consumer organizations, but methods for independent control of catch certificates and eco-labels are urgently needed. Here we show that, by using gene-associated single nucleotide polymorphisms, individual marine fish can be assigned back to population of origin with unprecedented high levels of precision. By applying high differentiation single nucleotide polymorphism assays, in four commercial marine fish, on a pan-European scale, we find 93-100% of individuals could be correctly assigned to origin in policy-driven case studies. We show...
Tipo: Text
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00090/20085/17722.pdf

Evaluation of the impact of polyethylene microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mazurais, David; Ernande, Bruno; Quazuguel, Patrick; Severe, Armelle; Huelvan, Christine; Madec, Lauriane; Mouchel, Olivier; Soudant, P.; Robbens, J.; Huvet, Arnaud; Zambonino-infante, J.
Microplastics are present in marine habitats worldwide and may be ingested by low trophic organisms such as fish larvae, with uncertain physiological consequences. The present study aims at assessing the impact of polyethylene (PE 10-45µM) microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae. Fish were fed an inert diet including 0, 104 and 105 fluorescent microbeads per gram from 7 until 43 days post-hatching (dph). Microbeads were detected in the gastrointestinal tract in all fish fed diet incorporating PE. Our data revealed an efficient elimination of PE beads from the gut since no fluorescent was observed in the larvae after 48h depuration. While the mortality rate increased significantly with the amount of microbeads scored per...
Tipo: Text
Palavras-chave: Microplastic; Fish; Larvae; Ingestion; Survival.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00279/39057/37621.pdf

Nutritional programming by dietary carbohydrates in European sea bass larvae: not always what expected at juvenile stage
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zambonino-infante, Jose-luis; Panserat, S.; Servili, Arianna; Mouchel, Olivier; Madec, Lauriane; Mazurais, David.
Optimizing the metabolic use of these carbohydrate-rich plant products in fish feeds could be achieved through nutritional programming strategies of fish larvae. The present study aims at testing the long-term effect of an early-life high-carbohydrate nutritional (34% of starch in the feed) stimulus in European sea bass (Dicentrarchus labrax), by examining several biochemical and molecular parameters involved in carbohydrate metabolism in sea bass larvae and juveniles, compared to a control group fed standard larval regime (phase 1). As awaited, specific activities of digestive enzymes, from pancreatic and intestinal segments, involved in glucide digestion (amylase, sucrase) were enhanced together with an up-regulation of glucokinase gene and...
Tipo: Text
Palavras-chave: Dicentrarchus labrax; Glucose metabolism; High-carbohydrate regimes; Marine fish larvae; Nutritional programming.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00470/58129/60552.pdf

A new species of Phyllochaetopterus (Annelida: Chaetopteridae) from deep-sea hydrothermal Ashadze-1 vent field, Mid-Atlantic Ridge: taxonomical description and partial COI DNA sequence
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Morineaux, Marie; Nishi, Eijiroh; Ormos, Andrea; Mouchel, Olivier.
Phyllochaetopterus polus, a new species of the polychaete family Chaetopteridae is described. Specimens were collected at the Ashadze-1 vent field (Mid-Atlantic Ridge), from 4100-4200 m depth. P polus is the first chaetopterid reported from the Atlantic hydrothermal vents. Three chaetopterid species have been recorded from deep-sea hydrothermal vents belonging to two genera, Spiochaetopterus and Phyllochaetopterus. P polus sp. nov. is a medium-sized chaetopterid, characterized by 9 chaetigers in region A and 2 in region B; region C is usually incomplete in the specimens examined, but has at least 40-70 chaetigers. This polychaete has one pair of modified chaetae in chaetiger 4 with a pear-shaped obliquely truncated head, triangular uncini and a branched...
Tipo: Text
Palavras-chave: Polychaeta; Hydrothermal vent; Mid-Atlantic Ridge; Taxonomy; New species; COI.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00013/12439/9250.pdf

Sea bass Dicentrarchus labrax nervous necrosis virus isolates with distinct pathogenicity to sea bass larvae
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Breuil, Gilles; Mouchel, Olivier; Fauvel, Christian; Pepin, Jean-francois.
Reproduction of nodavirus disease was performed by experimental infection of sea bass eggs during fertilization or at larval stage 4 with 2 genetically distinguishable nodavirus strains (Sb1 and Sb2) isolated from sea bass collected along the Atlantic and Mediterranean French coast. The pathogenicity of the virus strains was assigned after detection of the virus by ELISA and immunohistochemistry (IHC). The Atlantic (Sb1) strain was more pathogenic than the Mediterranean (Sb2) strain during the fertilization step whilst both strains were pathogenic following experimental exposure of 4 d old larvae. Virus lesions developed in the brain 4 to 6 d following experimental exposure. Experimental ELISA proved very sensitive for detecting the nodavirus in Sb1 or Sb2...
Tipo: Text
Palavras-chave: ELISA; Nodavirus; Fish encephalopathy.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-814.pdf

Contribution au développement et à l'étude de marqueurs génétiques chez la crevette Penaeus stylirostrls
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mouchel, Olivier.
Genetic markers are actually developed in a number of research applications. They constitute promising tools when used in the field of genetic improvment of cultured aquatic species. The work presented in this report aims to show the end of the developpment of this kind of tool at the IFREMER Pacific Oceanologic Center. 6 markers (5 microsatellites and 1 intronic) have been selected and applied to genetic variability analysis and family connections recognizing. Our results show the possibility to realize this type of analysis at the IFREMER Pacific Oceanologic Center, and fUl1hermore the deterioration of the allelic diversity in the tahitian strains following spontaneous domestication without genetic consideration. Thanks to this work, pertinence of...
Tipo: Text
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00430/54174/55509.pdf

Climate change impacts on fish reproduction are mediated at multiple levels of the brain-pituitary-gonad axis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Servili, Arianna; Canario, Adelino V.m.; Mouchel, Olivier; Muñoz-cueto, José Antonio.
Anthropogenic emissions of carbon dioxide in the atmosphere have generated rapid variations in atmospheric composition which drives major climate changes. Climate change related effects include changes in physico-chemical proprieties of sea and freshwater, such as variations in water temperature, salinity, pH/PCO2 and oxygen content, which can impact fish critical physiological functions including reproduction. In this context, the main aim of the present review is to discuss how climate change related effects (variation in water temperature and salinity, increases in duration and frequency of hypoxia events, water acidification) would impact reproduction by affecting the neuroendocrine axis (brain-pituitary-gonad axis). Variations in temperature and...
Tipo: Text
Palavras-chave: Climate change; Fish reproduction; Gonads; Neuroendocrine regulation; Environmental control; Temperature.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00609/72079/70796.pdf

Recherche de moyens de dépistage et de prévention de la nodavirose du bar
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Coeurdacier, Jean-luc; Breuil, Gilles; Pepin, Jean-francois; Mouchel, Olivier; Fauvel, Christian; Laporte, Florent; Thiery, Richard; Castric, J; Raymond, Jean-christophe; Baudin Laurencin, Félix.
Ce rapport clôture une collaboration, entreprise courant 97, entre l’Agence Nationale pour la Valorisation de la Recherche (ANVAR), l’Agence Française de Sécurité Sanitaire des Aliments(AFSSA), le Syndicat National des Aquaculteurs Marins (SFAM) et l’Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER). Deux lignées de nodavirus infectant le bar ont été mises en évidence avec une pathogénie variable selon l'âge et les facteurs extérieurs. Ce virus s’est montré d’une résistance exceptionnelle aux facteurs tant physiques que chimiques. Les diagnostics existants ont été adaptés ou réabordés sous un angle différent. Une étude épidémiologique a été réalisée chez les producteurs. La vaccination par peptides synthétiques a permis de sélectionner...
Tipo: Text
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00167/27874/26120.pdf

The highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia resistance of sea bass, Dicentrarchus labrax, submitted to different nutritional histories
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gatesoupe, Francois-joel; Huelvan, Christine; Le Bayon, Nicolas; Le Delliou, Herve; Madec, Lauriane; Mouchel, Olivier; Quazuguel, Patrick; Mazurais, David; Zambonino-infante, Jose-luis.
Background  The better understanding of how intestinal microbiota interacts with fish health is one of the key to sustainable aquaculture development. The present experiment aimed at correlating active microbiota associated to intestinal mucosa with Specific Growth Rate (SGR) and Hypoxia Resistance Time (HRT) in European sea bass individuals submitted to different nutritional histories: the fish were fed either standard or unbalanced diets at first feeding, and then mixed before repeating the dietary challenge in a common garden approach at the juvenile stage. Results  A diet deficient in essential fatty acids (LH) lowered both SGR and HRT in sea bass, especially when the deficiency was already applied at first feeding. A protein-deficient diet with high...
Tipo: Text
Palavras-chave: Host-microbe interaction; 16S rRNA; Pyrosequencing; Autochthonous bacteria; Alternative feed ingredients; Physiological status.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00356/46719/46590.pdf

Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Sevilla, Rafael G.; Diez, Amalia; Noren, Michael; Mouchel, Olivier; Jerome, Marc; Verrez-bagnis, Veronique; Van Pelt, Hilde; Favre Krey, Laurence; Krey, Grigorios; Bautista, José M.
This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.
Tipo: Text
Palavras-chave: Teleost; Rhodopsin; PCR primers; Mitochondrial cytochrome b; Fish; DNA barcoding.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3034.pdf