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Trypan blue exclusion assay by flow cytometry
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Avelar-Freitas,B.A.; Almeida,V.G.; Pinto,M.C.X.; Mourão,F.A.G.; Massensini,A.R.; Martins-Filho,O.A.; Rocha-Vieira,E.; Brito-Melo,G.E.A.
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating cell viability that combines the TB exclusion test and the high sensitivity of the flow cytometry technique. Previous studies have demonstrated the ability of TB to emit fluorescence when complexed with proteins. According to our results, TB/bovine serum albumin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypan blue; Fluorescence; Flow cytometry; Cytotoxicity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400307