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Detection of Salmonella sp. from porcine origin: a comparison between a PCR method and standard microbiological techniques
Provedor de dados: 58
Autores: Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa.
The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection; PCR; Pork; Salmonella; Swine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013