Consulta: creatorFacets:"Mundo,Silvia Leonor"
Registros recuperados: 2
Data/hora: 13/06/2024 01:26:27

Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR
Provedor de dados: Brazilian Journal of Microbiology
Autores: Gilardoni,Liliana Rosa; Fernández,Bárbara; Morsella,Claudia; Mendez,Laura; Jar,Ana María; Paolicchi,Fernando Alberto; Mundo,Silvia Leonor.
Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samples from 265 lactating Holstein cows from Map infected and uninfected herds in Argentina. IMS-IS1 PCR results were analyzed and compared with those obtained from milk and fecal culture and serum ELISA. The extent of agreement between both tests was determined by the Kappa test. IMS-IS1 PCR showed a detection limit of 101 CFU of Map/mL of milk, when 50:50 mix of monoclonal and polyclonal antibodies were used to coat magnetic beads. All of the 118 samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium avium subsp. paratuberculosis; Immunomagnetic separation; PCR; Cow's milk.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200506

Bovine response to lipoarabinomannan vaccination and challenge with Mycobacterium paratuberculosis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Jolly,Ana; Morsella,Claudia; Bass,Laura; Fiorentino,María Andrea; Paolicchi,Fernando Alberto; Mundo,Silvia Leonor.
This study aimed to evaluate the immune response in bovines following immunization with a mycobaterial Lipoarabinomannan extract (LAMe) and the effect of Map challenge. LAMe vaccine induced specific antibody levels that diminished after the challenge and affected Map excretion at least for 100 days thereafter.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Paratuberculosis; Bovine; LAM; Humoral immune response; Experimental challenge.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200029