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AFLP and SRAP markers linked to the mj gene for root-knot nematode resistance in cucumber
Provedor de dados: Scientia Agricola
Autores: Devran,Zübeyir; Firat,Ahmet Fikret; Tör,Mahmut; Mutlu,Nedim; Elekçioğlu,Ibrahim Halil.
Root-knot nematodes (Meloidogyne spp.) are an important worldwide pest of cucumber (Cucumis sativus L.). Molecular markers linked to the Javanese root-knot nematode (M. javanica) resistance gene mj in cucumber may aid marker assisted selection. One-hundred AFLP (EcoRI-MseI) and 112 SRAP were used to screen resistant and susceptible parents for polymorphisms to develop molecular markers linked to the mj gene. Of the 100 AFLP primers, 92 produced bands and two yielded candidate markers (E-ATT/M-CAA and E-AAC/M-CTG). These two bands were cut off from polyacrylamide gel, cloned and sequenced. Primers designed from the sequences did not yield polymorphic bands between the parents. In addition, the sequences did not contain any restriction site or indel to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meloidogyne javanica; Resistance; Molecular breeding.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000100017