Consulta: creatorFacets:"Nagasawa, Hideyuki"
Registros recuperados: 25
Data/hora: 29/05/2024 00:56:36

Role of a 65-kDa Heat Shock Protein in Protective Immunity against Toxoplasma Infection
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Himeno, Kunisuke; Nagasawa, Hideyuki; Hisaeda, Hajime.
Palavras-chave: Toxoplasma gondii; HSP65; Protective immunity.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/189

Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Hara, Olgga A; Liao, Min; Baticados, Waren; Bannai, Hiroshi; Zhang, Guohong; Zhang, Sofa; Lee, Eung-goo; Nishikawa, Yoshifumi; Claveria, Florencia G.; Igarashi, Makoto; Nagasawa, Hideyuki; Xuan, Xuenan.
Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were...
Palavras-chave: Canine; ELISA; Neospora caninum; NcGRA7.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/995

Exposure to 50-Hz electric fields on stress response initiated by infection with the protozoan parasite, Toxoplasma gondii, in mice
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Hori, Takuya; Harakawa, Shinji; Inoue, Noboru; Nagasawa, Hideyuki; Kariya, Tatsuya.
The present study was aimed to examine whether a 50-Hz electric field (EF) affects biological stress responses. Male C57BL/6J mice were exposed to either 50-Hz EF (45 kV/m) or given a sham exposure for 30 minutes per day for 1, 2 or 4 weeks and then either mock inoculated or inoculated with the protozoan parasite, Toxoplasma gondii (T. gondii) PLK strain. Survival rate was calculated in mice infected with T. gondii after exposure to EF and the serum antibody levels to heat shock protein 60 (Hsp-60), and mRNA levels for Hsp-60, IFN-γ and IL-12p35 in mouse peripheral leucocytes were determined. As a result, no statistically significant difference was found in survival rate between mice exposed to EF and those in the sham exposure group. When mice were...
Palavras-chave: 50-Hz; Electric field; Hsp-60; Stress response; Survival rate.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/997

Different Activation of Lymphocytes in Reaction to Babesia microti Infection in iNOS-/- and Wildtype Mice
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Remer, Katharina; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行.
Palavras-chave: Lymphocytes; Babesia microti; Spleen; INOS-/- mice; CD4+ cells; CD8+ cells; IFN-γ; Antibody-titer.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/263

Characterization of P15 Antigen of Cryptosporidium parvum Expressed by a Recombinant Vaccinia Virus
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Xuan, Xuenan; Zhang, Sofa; Kamio, Tsugihiko; Tsushima, Y.; Kamada, Takenori; Nishikawa, Yoshifumi; Otsuka, Haruki; Karanis, Panagiotis; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; 玄, 学南; 西川, 義文; 五十嵐, 郁男.
Palavras-chave: C. parvum; P15; Vaccinia virus; Subunit vaccine.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/314

Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi,  and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction（PCR）method．The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001％. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281

Production of Transgenic Mice Carrying p30 Gene Encoding Major Surface Antigen of Toxoplasma gondii
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Inoue, Noboru; Igarashi, Ikuo; Nagasawa, Hideyuki; Horiuchi, Motohiro; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 五十嵐, 郁男; 長澤, 秀行.
Palavras-chave: Toxoplasma gondii; SAG-1 (p30); Transgenic mice.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/254

Preliminary Survey on Horse Serum Indirect Fluorescence Antibody Titers in Japan against Babesia equi and Babesia caballi (Onderstepoort strain) Antigen
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Suzuki, Naoyoshi; Igarashi, Ikuo; Abgaandorjiin, A.; Waal, D. T. De; Nagasawa, Hideyuki; Omata, Yoshitaka; Saito, Atsushi; Oyamada, Takashi.
Palavras-chave: Babesia caballi; Babesia equi; Sera diagnosis; IFAT; Survey.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/243

Expression of 65 000 MW Heat-shock Protein in SCID Mice Infected with Toxoplasma gondii after Transplantation of Mouse Fetal Thymus
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Nagasawa, Hideyuki; Hisaeda, Hajime; Ishikawa, Hiroyuki; Maekawa, Yoichi; Sakai, Tohru; Himeno, Kunisuke; 長澤, 秀行.
Palavras-chave: Heat shock protein; HSP65; Toxoplasma gondii; Protozoa; SCID mice; Protective immunity.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/229

Cooperation between the Japan International Cooperation Agency and the Obihiro University of Agriculture and Veterinary Medicine
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Nagasawa, Hideyuki; 長澤, 秀行.
Palavras-chave: JICA; Food safety; Food security; Animal Hygiene; Food Hygiene.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/927

Construction of Recombinant Feline Herpesvirus Type 1 Expressing Toxoplasma gondii SRS1
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Mishima, Masayuki; Xuan, Xuenan; Takeiri, Akira; Makala, Levi H. C; Igarashi, Ikuo; Fujisaki, Kozo; Nagasawa, Hideyuki; Mikami, Takeshi.
Palavras-chave: Toxoplasma; Vaccine; SRS1; FHV.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/343

CHARACTERIZATION OF CD11c+ DENDRITIC CELL POPULATIONS IN SPLEENS OF MICE INFECTED WITH TOXOPLASMA GONDII
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Makala, Levi H. C; Zayatiin, Batsukh; Kamada, Takenori; Seng, Seyha; Ribas, Lazaro; Mishima, Masayuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Nagasawa, Hideyuki.
Palavras-chave: Spleen; Dendritic cell; Mouse; Toxoplasma lysate antigen (TLA).
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/625

Non-invasive Method of Identification of SAG-1 Transgenic Mice by PCR Analysis of Oral Wash Cells
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Suzuki, R.; Kato, Mihoko; Bray, R. L.; Lim, C.; Zayatiin, Batsukh; Kamada, Takenori; Inoue, Noboru; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 玄, 学南.
Palavras-chave: SAG-1; Oral wash and two-step polymerase chain reaction.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/311

CLONING AND EXPRESSION OF AN ANTIGEN OF BABESIA GIBSONI IN ESCHERICHIA COLI AND ITS USE FOR THE IMMUNODIAGNOSIS OF CANINE BABESIOSIS
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志.
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624

Kinetoplast DNA and Procyclic Acidic Repetitive Protein A-α Gene of Trypanosoma evansi
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Inoue, Noboru; Honzako, Y.; Hirumi, K.; Xuan, Xuenan; Agatsuma, T.; Nagasawa, Hideyuki; Mikami, Takeshi; Hirumi, Hiroyuki.
Palavras-chave: PARP; KDNA; Trypanosoma evansi; T. brucei.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/276

Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, p23
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Takashima, Yasuhiro; Xuan, Xuenan; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Igarashi, Ikuo; Fujisaki, Kozo; Mikami, Takeshi; Otsuka, Haruki; 玄, 学南; 五十嵐, 郁男.
To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the...
Palavras-chave: Cryptosporidium parvum; P23; Herpes; Pseudorabies virus; Subunit vaccine.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/129

IMMUNOGENIC PROPERTY OF A RECOMBINANT VACCINIA VIRUS EXPRESSING P23 OF CRYPTOSPORIDIUM PARVUM
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Takashima, Yasuhiro; Xuan, Xuenan; Hongo, Maya; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Otsuka, Haruki.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/632

The Role of Scavenger Receptor Type A (SR-A) during Infection with Babesia microti
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Kamada, Takenori; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Suzuki, Hiroshi; Kodama, Tatsuhiko; Suzuki, Naoyoshi; Toyoda, Yutaka; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行; 鈴木, 宏志.
Palavras-chave: Babesiosis; Protective immune response; Knock-out mouse; SRKO.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/265

Epidemiological survey of Babesia gibsoni infection in dogs in eastern Japan
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Miyama, Takako; Sakata, Yoshimi; Shimada, Yojiro; Ogino, Shoji; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; Nagasawa, Hideyuki; Inokuma, Hisashi.
To determine the distribution of Babesia gibsoni infection in dogs in the eastern part of Japan, an epidemiological survey of dogs suspected of having B. gibsoni infection was attempted using the polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Thirty-five of 115 such dogs (30.4%) were positive by PCR and/or ELISA. The 35 positive dogs consisted of 28 Tosa dogs, 4 American Pit Bull Terriers, and 3 mongrel dogs in Aomori, Fukushima, Ibaraki, Gunma, Chiba, Tokyo, Kanagawa, and Nagano Prefectures. The positive dogs had a significantly lower rate of tick exposure and a higher rate of bites by other dogs. Twenty-two of 35 B. gibsoni-positive dogs were infected with hemoplasma, and the rate of infection was significantly higher than...
Palavras-chave: Babesia gibsoni; Eastern Japan; Enzyme-linked immunosorbent assay; Hemoplasma; Polymerase chain reaction.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/924

A PRELIMINARY STUDY FOR ELIMINATION OF CONTAMINATED BABESIA MICROTI FROM THE MODEL OF HUMAN PLASMA FRACTION
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Nishisaka, M.; Igarashi, Ikuo; Fujisaki, Kozo; Miyahara, T.; Ogata, Y.; Nagasawa, Hideyuki; Mikami, Takeshi.
Palavras-chave: Babesia microti; Human plasma fraction; Filtration.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/629

Role of heat shock proteins in protective immunity against infection with Toxoplasma Gondii
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Suzuki, Naoyoshi; Nagasawa, Hideyuki; Hisaeda, Hajime; Igarashi, Ikuo; Horiuchi, Motohiro; Inoue, Noboru; Himeno, Kunisuke.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1061

Sequence Analysis of Three Major Antigens (P30, P23 and P22) of Virulent and Avirulent Strains of Toxoplasma gondii
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Maki, Yoshiyuki; Seng, Seyha; Kato, Mihoko; Hoshi, Yukihiro; Igarashi, Ikuo; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi.
Palavras-chave: Toxoplasma gondii; SAG-1 (P30); CDNA.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/250

DENDRITIC CELLS AND HOST RESISTANCE TO TOXOPLASMA GONDII
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Makala, Levi H. C.; Nagasawa, Hideyuki.
Palavras-chave: Toxoplasma gondii; IL-12; IFN-γ; Dendritic cells; Host resistance; Mouse.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/634

Detection of Antibodies to Babesia equi by The Latex Agglutination Test with Recombinant Merozoite Antigen-2
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Tanaka, Tetsuya; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; EMA-2; Baculovirus; Latex agglutination test.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/329

Purification of Merozoites from In Vitro Cultured Babesia caballi- Infected Equine Erythrocytes
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Ikadai, Hiromi; Sato, Masumi; Avarzed, Abgaandorjiin; Nagasawa, Hideyuki; Igarashi, Ikuo; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; 長澤, 秀行; 五十嵐, 郁男.
Palavras-chave: Purification; Merozoite; Babesia caballi.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/272