Consulta: creatorFacets:"Nardi,Nance Beyer"
Registros recuperados: 5
Data/hora: 10/06/2024 08:13:22

Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Mayra Ramos de Jesus; Pinhatti,Valéria Rodrigues; Silveira,Maiele Dornelles da; Matzenbacher,Cristina Araujo; Freitas,Thales Renato Ochotorena de; Silva,Juliana da; Camassola,Melissa; Nardi,Nance Beyer.
Abstract Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil. Cultures showed typical morphology and proliferation potential. Adipose-derived MSCs showed trilineage potential. Cultures derived from adipose tissue, bone marrow and brain were immunophenotyped with negative results for CD31, CD44, CD45,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem/stromal cells; Tuco-tuco; Ctenomys minutus.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500870

A role for adeno-associated viral vectors in gene therapy
Provedor de dados: Genetics and Molecular Biology
Autores: Coura,Renata dos Santos; Nardi,Nance Beyer.
Gene therapy constitutes a therapeutic intervention based on modification of the genetic material of living cells, by correcting genetic defects or overexpressing therapeutic proteins. The success of gene therapy protocols depends on the availability of therapeutically suitable genes, appropriate gene delivery systems and proof of safety and efficacy. Recent advances on the development of gene delivery systems, particularly on viral vectors engineering and improved gene regulatory systems, have led to marked progress in this field. Although the available vector systems can successfully transfer genes into cells, the ideal delivery vehicle has not been found. In this context, adeno-associated virus vectors (AAV) are arising as a promising tool for a wide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adeno-associated virus; AAV-based recombinant vectors; Gene therapy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100001

Human mesenchymal stem cells are resistant to cytotoxic and genotoxic effects of cisplatin in vitro
Provedor de dados: Genetics and Molecular Biology
Autores: Bellagamba,Bruno Corrêa; Abreu,Bianca Regina Ribas de; Grivicich,Ivana; Markarian,Carolina Franke; Chem,Eduardo; Camassola,Melissa; Nardi,Nance Beyer; Dihl,Rafael Rodrigues.
Abstract Mesenchymal stem cells (MSCs) are known for their important properties involving multilineage differentiation potential., trophic factor secretion and localization along various organs and tissues. On the dark side, MSCs play a distinguished role in tumor microenvironments by differentiating into tumor-associated fibroblasts or supporting tumor growth via distinct mechanisms. Cisplatin (CIS) is a drug widely applied in the treatment of a large number of cancers and is known for its cytotoxic and genotoxic effects, both in vitro and in vivo. Here we assessed the effects of CIS on MSCs and the ovarian cancer cell line OVCAR-3, by MTT and comet assays. Our results demonstrated the resistance of MSCs to cell death and DNA damage induction by CIS,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mesenchymal stem cells; Cisplatin; MTT; Comet assay; OVCAR-3 cells.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100129

Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
Provedor de dados: Genetics and Molecular Biology
Autores: Wits,Maria Inês; Tobin,Gabriela Cabanas; Silveira,Maiele Dornelles; Baja,Karine Gehlen; Braga,Luisa Maria Macedo; Sesterheim,Patricia; Camassola,Melissa; Nardi,Nance Beyer.
Abstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stromal cells; Hyaluronic acid; Osteoarthritis; Dog.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100701

Beyond retrovirus infection: HIV meets gene therapy
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Flávia Helena da; Dalberto,Tiago Pires; Nardi,Nance Beyer.
The human immunodeficiency virus (HIV) is classified as a retrovirus because of its RNA genome and the fact that it requires reverse transcriptase to convert it into DNA. This virus belongs to the lentivirinae subfamily and is able to infect quiescent cells but is better known for its association with acquired immunodeficiency syndrome (AIDS) and can be described as one of the most effective vectors for gene transfer. Biosafety concerns are present whenever viral vectors are employed but are particularly pertinent to the development of HIV-based vectors. Insertional mutagenesis and the production of new replication-competent viruses (RCV) have been pointed to as major problems, but experimental data have shown that safe protocols can be developed for their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; MLV; Retrovirus; Lentivirus; Gene therapy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200027