Consulta: creatorFacets:"Nehme,P"
Registros recuperados: 1
Data/hora: 08/06/2024 17:26:45

Development of an enzyme-linked immunosorbent assay for quantification of Salmonella enteritidis-specific antibodies in egg yolk
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Tayeb,IT; Nehme,P; Jaber,L; Barbour,EK.
The present study aims at developing an indirect ELISA to quantify yolk antibodies specific to all surface proteins of the invasive Salmonella Enteritidis (SE), which acquired the 1.8, 14.1, and ~ 50 Kb plasmids. An ELISA checkerboard was used in four different experiments to account for the different parameters included in the preliminary ELISA procedure, and consequently to maximize the difference in Optical Density (OD) values between control positive and negative yolk samples. The first experiment aimed at studying the impact of 5% Bovine Serum Albumin (BSA) dissolved in distilled water as a blocking reagent on a 28 µg/well SE antigen-coated plate, while applying the positive and negative control yolk samples to different concentrations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Egg yolk; ELISA; Salmonella Enteritidis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400009