Consulta: creatorFacets:"Nichi,M."
Registros recuperados: 3
Data/hora: 02/06/2024 11:45:08

The use of reduced glutathione (GSH) as antioxidant for cryopreserved sperm in dogs
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Angrimani,D.S.R.; Nichi,M.; Brito,M.M.; Kawai,G.K.V.; Rui,B.R.; Losano,J.D.A.; Vieira,N.M.G.; Francischini,M.C.P.; Cruz,D.S.G.; Queiroz-Hazarbassanov,N.; Massoco,C.O.; Vannucchi,C.I.
ABSTRACT The aim of this study was to evaluate the effect of supplementation with different concentrations of reduced glutathione GSH (0; 5; 7.5; 10mM) in the extender for cryopreservation in dogs with evaluations performed after glycerolization (chilled) and thawing (thawed). For this purpose, we used 8 dogs and two semen collections were performed in a weekly interval, totaling 16 semen samples. The sperm were analyzed by automatic sperm motility (CASA) and flow cytometry analysis of mitochondrial potential (JC1 dye) and membrane/acrosome integrity (FITC-PI dyes). We evaluated subjectively the membrane and acrosome integrity, mitochondrial activity and DNA integrity. Seminal plasma was evaluated for lipid peroxidation (TBARS concentration). Chilled and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reactive oxygen species; Cryopreservation; Antioxidant; Canine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200419

The influence of canine brucellosis on morphofunctional features of epididymal spermatozoa: case report
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Angrimani,D.S.R.; Lúcio,C.F.; Losano,J.D.A.; Brito,M.M.; Silva Júnior,R.A.; Keid,L.B.; Nichi,M.; Vannucchi,C.I.
ABSTRACT The present work reports a clinical case of a mongrel dog, with serological diagnosis of brucellosis, from which epididymal sperm analysis was performed. Sperm samples were collected from different segments of the epididymis (tail, corpus, and caput). Sperm samples were evaluated for computer-assisted motility analysis (CASA), spermatic morphology, mitochondrial activity and sperm plasmatic membrane and acrosomal integrity. Changes in sperm movement patterns were found (progressive motility, percentage of rapid sperm, percentage of rapid velocity, average pathway, curvilinear velocity, velocity straight line, amplitude of lateral head displacement, straightness and linearity), increase of total morphological defects (51%) and absence of sperm...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Brucella canis; Epididymal sperm.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601449

Gene expression of estrogen and oxytocin receptors in the uterus of pregnant and parturient bitches
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Veiga,G.A.L.; Milazzotto,M.P.; Nichi,M.; Lúcio,C.F.; Silva,L.C.G.; Angrimani,D.S.R.; Vannucchi,C.I.
In the canine species, the precise mechanisms of pregnancy maintenance and the initiation of parturition are not completely understood. The expression of genes encoding the receptors for estrogen (ERα mRNA) and oxytocin (OTR mRNA) was studied in the endometrium and myometrium during pregnancy and parturition in dogs. Real-time PCR was performed to quantify the levels of ERα mRNA and OTR mRNA in the uterus of bitches during early (up to 20 days of gestation), mid (20 to 40 days) and late pregnancy (41 to 60 days), and parturition (first stage of labor). All tissues expressed ERα and OTR mRNA, and are thus possibly able to respond to eventual estrogen and oxytocin hormonal stimuli. No statistically significant differences in the expression of ERα mRNA were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine; Estrogen; Oxytocin; Receptors; Pregnancy; Parturition.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400339