Consulta: creatorFacets:"Niel,C."
Registros recuperados: 2
Data/hora: 09/06/2024 05:36:32

A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
Provedor de dados: 56
Autores: Devalle,S.; Niel,C.
Torque teno virus (TTV) is a circular, single-stranded DNA virus that chronically infects healthy individuals of all ages worldwide. TTV has an extreme genetic heterogeneity which is reflected in its current classification into five main phylogenetic groups (1-5). Using specific PCR assays, it has been shown that many individuals are co-infected with TTV isolates belonging to different phylogenetic groups. Here, a multiplex PCR assay was developed, using five recombinant plasmids. Each plasmid carried an insert of different size issued from a TTV isolate belonging to a different group. The assay was able to simultaneously amplify DNAs of TTV isolates belonging to all five phylogenetic groups. Multiplex PCR was then tested satisfactorily on DNAs extracted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Co-infections; Multiplex; PCR; Torque teno virus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600006

A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus
Provedor de dados: 56
Autores: Moraes,M.T.B.; Niel,C.; Gomes,S.A.
We have developed a polymerase chain reaction (PCR) assay which distinguishes genotype F from the other genotypes of hepatitis B virus (HBV). The method was used to characterize HBV strains isolated in urban areas of the Brazilian Amazon. DNA was amplified in 54 of a total of 78 HBsAg-positive serum samples, using universal, non-genotype-specific primers. Only 4 (7.4%) were identified as genotype F by our genotype-specific PCR assay. This proportion is notably lower than that previously reported in Argentina, Venezuela, Peru, and Central America.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis B virus; Genotype F; Subtype adw4; Polymerase chain reaction; Brazil.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100006