Consulta: creatorFacets:"Ninhaus-Silveira,Alexandre"
Registros recuperados: 7
Data/hora: 09/06/2024 18:35:10

Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol
Provedor de dados: Neotropical Ichthyology
Autores: Carneiro-Leite,Laicia; Bashiyo-Silva,Cristiane; Oliveira,Yasmim Alves Araújo; Borges,Laís Pedroso; Sanchez,Malbelys Padilla; Silva,Luciane Gomes da; Lobato,Stella Indira Rocha; Rodrigues-Lisoni,Flávia Cristina; Veríssimo-Silveira,Rosicleire; Ninhaus-Silveira,Alexandre.
Abstract This study aimed to determine the semen characteristics of Astyanax lacustris after hormonal induction and to evaluate the sensitivity of the species sperm to cryoprotective solutions based on the cryoprotectants dimethyl sulfoxide and methyl glycol. Volume, color, sperm concentration, total motility and aspects of sperm movement were analyzed using “Integrated Semen Analysis System”. Three different extenders were tested: A) glucose 5%+egg yolk 10%, B) BTS®5% and C) glucose 5% and two permeable cryoprotectants: dimethyl sulfoxide (Me2SO) and methyl glycol (MTG). Fresh A. lacustris semen presented total motility of 76.6±11.2%, motility duration of 33.0±2.2s, sperm concentration of 7.22±3.2×109sptz/mL and seminal osmolality of 219±0.03mOsm/kg-1....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characiformes; DNA integrity; Freezing; Semen quality; Yellow-tailed Lambari.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000300214

Morphology of the urogenital papilla and its component ducts in Astyanax altiparanae Garutti &amp;amp; Britski, 2000 (Characiformes: Characidae)
Provedor de dados: Neotropical Ichthyology
Autores: Siqueira-Silva,Diógenes Henrique de; Silva,Amanda Pereira dos Santos; Ninhaus-Silveira,Alexandre; Veríssimo-Silveira,Rosicleire.
The histological description of the urogenital papilla is an important tool to comprehension of the reproductive mechanisms in fish, as well as a pre-requisite to germ cell transplantation in adult fish, besides to be a good biological indicator to environmental changes. Was performed the histological description of the urogenital papilla and its component ducts in the tetra Astyanax altiparanae. The genital and urinay ducts pass separately throughout most part of its extension, joining in a single duct before opening. In males this opening is asymmetric and seems to have double origin, being completely surrounded by striated muscle fibers, while in females it is symmetric and the muscle fibers does not surround it totally. Spermatic duct and oviduct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish reproduction; Histological analysis; Oviduct; Sperm duct; Urinary duct.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000200309

Structural analysis of the Pimelodus maculatus (Lacépède, 1803) embryogenesis (Siluriformes: Pimelodidae)
Provedor de dados: Neotropical Ichthyology
Autores: Buzollo,Hellen; Veríssimo-Silveira,Rosicleire; Oliveira-Almeida,Isângela R.; Alexandre,Juliana S.; Okuda,Hélio T.; Ninhaus-Silveira,Alexandre.
The fish embryonic development comprises the events between the egg fertilization up to larvae hatching, being useful for the identification of viable eggs in productivity and survival studies as well as in raising experiments of several species. The goal of the present study was to characterize the embryonic development of Pimelodus maculatus (Siluriformes; Pimelodidae). The embryogenesis was typical of teleosteans, but with differences in relation to other species such as duration of development, type of blastocoel, moment of somite segmentation among others. Six stages of embryonic development were defined: zygote, cleavage, blastula, gastrula, organogenesis (divided in phases: early segmentation and late segmentation) and hatching with a period of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryonic development; Induced spawning; Morphology; Yolk syncyctial layer.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000300013

Effects of cryoprotectants and low temperatures on hatching and abnormal embryo development of Prochilodus lineatus (Characiformes: Prochilodontidae)
Provedor de dados: Neotropical Ichthyology
Autores: Costa,Raphael S.; Souza,Fabrício M. S. de; Senhorini,José A.; Veríssimo-Silveira,Rosicleire; Ninhaus-Silveira,Alexandre.
ABSTRACT This study evaluated the effect of the cryoprotectants and the low temperatures on the embryonic development of Prochilodus lineatus, describing their main morphological alterations. On chilling sensitivity test, the survival rates at the twenty somites stage (20S) were 53.6% at 0ºC, and 100% in 5ºC. To test toxicity, the embryos were exposed to a graded series of 1,2-Propanediol (PROP), dimethyl sulfoxide (Me2SO4) and glycerol (GLY), terminating in a solution of high osmolarity. There was no significant difference in the embryos survival of toxicity test between series of PROP and Me2SO4 in the 6S and 20S. In the cooling protocols, were evaluated the effects of low temperature associated with cryoprotectants. At 5ºC, PROP showed survival rates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cooling; Cryopreservation; Injuries; Morphology; Toxicity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000300201

Reproductive cycle of the Neotropical cichlid yellow peacock bass Cichla kelberi: A novel pattern of testicular development
Provedor de dados: Neotropical Ichthyology
Autores: Siqueira-Silva,Diógenes Henrique de; Vicentini,Carlos Alberto; Ninhaus-Silveira,Alexandre; Veríssimo-Silveira,Rosicleire.
The present study describes the testicular maturation phases (associating the germ cells development and the morphological changes suffered by the germinal epithelium along the whole year), and the testicular morphology in the yellow peacock bass Cichla kelberi, relating it to other species. For this purpose, 78 specimens were studied according conventional techniques of light microscope. The testes in C. kelberi were classified as unrestricted spermatogonial lobular, an apomorphic characteristic in the recent groups of Teleost. Furthermore, were defined five testicular maturation phases: Preparatory phase; Early Germinal Epithelium Development; Mid Germinal Epithelium Development; Late Germinal Epithelium Development and; Regression. Similar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germinal epithelium; Gonadal maturation; Reproductive phases; Spermatogenesis; Testes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300587

Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869)
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Ninhaus-Silveira,Alexandre; Foresti,Fausto; Veríssimo-Silveira,Rosicleire; Senhorini,José Augusto.
Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of Brycon cephalus were performed. The seminal characteristics observed were: an almost transparent, milky semen with a mean volume of 4mL, and sperm concentration of 9.617±1.630 x 10(6) spermatozoa/mm³. Spermatozoa (length = 31.288 ±4.47µm) were of the aquasperm type and displayed a small, round head (length = 1.727 ±0.18 µm; width = 1.752 ±0.17µm) without acrosomal vesicle, nucleus with highly condensed chromatin forming coarse clots and centriolar complex located in the nuclear fossa; a midpiece (length = 2.561±0.44µm), narrowed rearward, with a cytoplasmic canal; and a flagellum (length = 29.521 ±4.37µm). Fertilization tests with thawed semen demonstrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Semen characteristics; Cryopreservation; Brycon cephalus; Ultrastructure; Fish.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500015

Cryopreservation of semen from functional sex-reversed genotypic females of the rainbow trout, Oncorhynchus mykiss
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Ninhaus-Silveira,Alexandre; Foresti,Fausto; Tabata,Yara Aiko; Rigolino,Marcos Guilherme; Veríssimo-Silveira,Rosicleire.
Cryopreservation of semen from sex-reversed females of rainbow trout aims at rationalizing the production of stocks composed by 100% females. Semen from normal males (M) and two types of genotypic females (R and G), sex-reversed by the oral administration of 17alpha-methyltestosterone, were used. R was obtained by the fertilization of normal eggs with semen of sex-reversed females while G via gynogenetic reproduction. Semen was diluted in an extender solution (glucose 5,4 g, egg yolk 10 ml, dimetil sulfoxide 10 ml, water 80 ml) at 1:3 ratio (semen/extender), stored in straws of 0.5 ml and freezed in a dry container Cryopac CP-65, at -180ºC. Thawing was performed with water at 70ºC for 3 seconds. There were no significant fertilization rate differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oncorhynchus mykiss; Cryopreservation; Sex reversal; Gynogenesis; Semen.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100009