Consulta: creatorFacets:"Oliveira,D.M."
Registros recuperados: 2
Data/hora: 08/06/2024 14:16:37

Antigenic stimulation is more efficient than LPS in inducing nitric oxide production by human mononuclear cells on the in vitro granuloma reaction in schistosomiasis
Provedor de dados: 56
Autores: Oliveira,D.M.; Silva-Teixeira,D.N.; Araújo-Filho,R.; Goes,A.M.
Nitric oxide (NO) is an extremely important and versatile messenger in biological systems. It has been identified as a cytotoxic factor in the immune system, presenting anti- or pro-inflammatory properties under different circumstances. In murine monocytes and macrophages, stimuli by cytokines or lipopolysaccharide (LPS) are necessary for inducing the immunologic isoform of the enzyme responsible for the high-output production of NO, nitric oxide synthase (iNOS). With respect to human cells, however, LPS seems not to stimulate NO production in the same way. Addressing this issue, we demonstrate here that peripheral blood mononuclear cells (PBMC) obtained from schistosomiasis-infected patients and cultivated with parasite antigens in the in vitro granuloma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; LPS; Schistosomiasis infection; In vitro granuloma.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100015

Characterization of alpha thalassemic genotypes by multiplex ligation-dependent probe amplification in the Brazilian population
Provedor de dados: 56
Autores: Suemasu,C.N.; Kimura,E.M.; Oliveira,D.M.; Bezerra,M.A.C.; Araújo,A.S.; Costa,F.F.; Sonati,M.F.
Alpha-thalassemia is the most common inherited disorder of hemoglobin synthesis. Genomic deletions involving the alpha-globin gene cluster on chromosome 16p13.3 are the most frequent molecular causes of the disease. Although common deletions can be detected by a single multiplex gap-PCR, the rare and novel deletions depend on more laborious techniques for their identification. The multiplex ligation-dependent probe amplification (MLPA) technique has recently been used for this purpose and was successfully used in the present study to detect the molecular alterations responsible for the alpha-thalassemic phenotypes in 8 unrelated individuals (3 males and 5 females; age, 4 months to 30 years) in whom the molecular basis of the disease could not be determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-thalassemia; Hb H disease; Multiplex ligation-dependent probe amplification; Genetic polymorphisms; Brazilian population.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100003