Consulta: creatorFacets:"Otsuka, Haruki"
Registros recuperados: 5
Data/hora: 10/06/2024 11:04:54

Characterization of P15 Antigen of Cryptosporidium parvum Expressed by a Recombinant Vaccinia Virus
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Xuan, Xuenan; Zhang, Sofa; Kamio, Tsugihiko; Tsushima, Y.; Kamada, Takenori; Nishikawa, Yoshifumi; Otsuka, Haruki; Karanis, Panagiotis; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; 玄, 学南; 西川, 義文; 五十嵐, 郁男.
Palavras-chave: C. parvum; P15; Vaccinia virus; Subunit vaccine.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/314

Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, p23
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Takashima, Yasuhiro; Xuan, Xuenan; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Igarashi, Ikuo; Fujisaki, Kozo; Mikami, Takeshi; Otsuka, Haruki; 玄, 学南; 五十嵐, 郁男.
To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the...
Palavras-chave: Cryptosporidium parvum; P23; Herpes; Pseudorabies virus; Subunit vaccine.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/129

DNA vaccine coding a p23 protein of Cryptosporidium parvum fused with Fc portion of immunogloblin G induces higher level of interferon-γ expression in mice
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Takashima, Yasuhiro; Xuan, Xuenan; Shirafuji, Hiroaki; Zhang, Guohong; Otsuka, Haruki.
To develop DNA vaccines against Cryptosporidiosis, a plasmid coding an immunodominant protein of Cryptosporidium parvum sporozoite, p23 (pCX-p23) and another plasmid coding a fusion protein containing whole the p23 and the Fc portion of mouse immunogloblin G1 (pCX-p23Fc). Vaccination of BALB/c mice with the plasmid, pCX-p23 and pCX-p23Fc induced the production of antibodies against p23. Although both of splenocytes of mice immunized with the plasmids pCX-p23 and pCX-p23Fc expressed interleukin-4 and interferon-8, after the in vitro stimulation by p23 antigen, the interferon-8 expression level of pCX-p23Fc immunized mice was much higher than that of pCX-p23 immunized mice. These results suggest a possibility  of the plasmid pCX-p23Fc as a DNA vaccine...
Palavras-chave: C. parvum; DNA vaccine; Fc; Interferon-8.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/147

IMMUNOGENIC PROPERTY OF A RECOMBINANT VACCINIA VIRUS EXPRESSING P23 OF CRYPTOSPORIDIUM PARVUM
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Takashima, Yasuhiro; Xuan, Xuenan; Hongo, Maya; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Otsuka, Haruki.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/632

IDENTIFICATION OF A  DOMINANT AND CYTOADHERENT VAR GENE EXPRESSED IN PLASMODIUM FALCIPARUM ITG PARASITES
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Morisawa, Aki; Basuki, Sukmawati; Arakaki, Nana; Otsuka, Haruki; Matsumoto, Yoshitsugu; Matsumoto, Yasunobu.
Palavras-chave: Plasmodium falciparum-infected erythrocyte membrane protein-1 (PfEMP-1); Cytoadhesion; Var gene.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/641