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Data/hora: 03/06/2024 04:36:15

High genetic diversity among Pseudomonas aeruginosa and Acinetobacter spp. isolated in a public hospital in Brazil
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Siqueira,Vera Lúcia Dias; Cardoso,Rosilene Fressatti; Pádua,Rubia Andreia Falleiros de; Caleffi-Ferracioli,Katiany Rizzieri; Helbel,Cesar; Santos,Adolfo Carlos Barreto; Aoki,Elisabeth Eyko; Nakamura,Celso Vataru.
In Brazil and other regions of the world, Pseudomonas aeruginosa and Acinetobacter spp. have emerged as important agents of nosocomial infection and are commonly involved in outbreaks. The main objective of the present study was to evaluate the genetic relationship among P. aeruginosa and Acinetobacter spp. isolated from patients in a public university hospital in northwestern Paraná, Brazil, and report their antimicrobial resistance profile. A total of 75 P. aeruginosa and 94 Acinetobacter spp. isolates were phenotypically identified and tested for antibiotic susceptibility using automated methodology. Polymyxin B was tested by disk diffusion for P. aeruginosa. Metallo-β-lactamase (MBL) was detected using a disk approximation test. Genotyping was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa/antimicrobial resistance profile; Pseudomonas aeruginosa/genetic study; Acinetobacter spp./antimicrobial resistance profile; Acinetobacter spp./genetic study; Antimicrobial resistance; Bacterial typing.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100006

High prevalence rate of extended-spectrum beta-lactamases (ESBL) among Enterobacteriaceae in a small Brazilian public hospital
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Lenhard-Vidal,Adriane; Cardoso,Rosilene Fressatti; Pádua,Rubia Andreia Falleiros de; Siqueira,Vera Lúcia Dias.
The production of extended-spectrum beta-lactamases (ESBL) is considered one of the most important resistance mechanisms that impair antimicrobial treatment of infections caused by Enterobacteriaceae. Data on culture and susceptibility tests were collected from the Clinical Analyses and Research Laboratory charts reporting on patients admitted to the University Hospital of Maringá (HUM) from January 2004 to December 2009. The following Enterobacteriaceae were selected: Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp. and Proteus mirabilis. All tests were performed according to the recommendations of the Clinical and Laboratory Standards Institute (biochemical identification; susceptibility tests; initial screening and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extended-spectrum β-lactamases; Enterobacteriaceae; Bacterial resistance; Β-lactams.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400006

β-lactamase-producing Gram-negative bacteria in an intensive care unit in southern Brazil
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Leite,Clariana Akemi Kariya; Oizumi,Karina Yoshimi; Caleffi-Ferracioli,Katiany Rizzieri; Scodro,Regiane Bertin de Lima; Pádua,Rubia Andreia Falleiros de; Cardoso,Rosilene Fressatti; Pires,Claudia Terencio Agostinho; Siqueira,Vera Lucia Dias.
ABSTRACT The present study evaluated the antimicrobial susceptibility profile, β-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and β-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Pseudomonas aeruginosa; Acinetobacter spp; Antimicrobial resistance; Beta-lactamases; Bacterial typing; Intensive Care Unit/study/Brazil; Drug-resistant bacteria.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200609