Consulta: creatorFacets:"Paiva,Edilson"
Registros recuperados: 16
Data/hora: 09/06/2024 11:49:11

Prospecting sugarcane genes involved in aluminum tolerance
Provedor de dados: Genetics and Molecular Biology
Autores: Drummond,Rodrigo D.; Guimarães,Claudia T.; Felix,Juliana; Ninamango-Cárdenas,Fernando E.; Carneiro,Newton P.; Paiva,Edilson; Menossi,Marcelo.
Aluminum is one of the major factors that affect plant development in acid soils, causing a substantial reduction in yield in many crops. In South America, about 66% of the land surface is made up of acid soils where high aluminum saturation is one of the main limiting factors for agriculture. The biochemical and molecular basis of aluminum tolerance in plants is far from being completely understood despite a growing number of studies, and in the specific case of sugarcane there are virtually no reports on the effects of gene regulation on aluminum stress. The objective of the work presented in this paper was to prospect the sugarcane expressed sequence tag (SUCEST) data bank for sugarcane genes related to several biochemical pathways known to be involved...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100029

Effect of Baculovirus spodoptera isolates in Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD
Provedor de dados: Neotropical Entomology
Autores: Barreto,Marliton R.; Guimaraes,Cláudia T.; Teixeira,Flávia F.; Paiva,Edilson; Valicente,Fernando H.
The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 10³ to 10(8) polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insect pathology; Fall armyworm larvae; PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100010

Rhizobium strains competitiveness on bean nodulation in Cerrado soils
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Raposeiras,Ruy; Marriel,Ivanildo Evódio; Muzzi,Maria Rita Scotti; Paiva,Edilson; Pereira Filho,Israel Alexandre; Carvalhais,Lilia Costa; Passos,Raul Vinícius Magalhães; Pinto,Patrícia Pereira; Sá,Nadja Maria Horta de.
The objective of this work was to identify the most competitive and effective Rhizobium strains in order to increase common bean yield by nitrogen fixation as alternative or complementation to the nitrogen fertilization. Competitiveness tests were lead in axenic conditions, in Cerrado soil pots and in three field experiments, with native Rhizobium strains that were previously identified, according to their effectiveness and genetic variability. The identification of strains in nodules was performed using serological tests (axenic conditions) - agglutination and enzyme linked immunosorbent (Elisa) assays - and random amplified polymorfic DNA (RAPD) (Cerrado soil). Plant yield was determined using the dry weight (greenhouse conditions), total N and grain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Nodule occupancy; Elisa; RAPD.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000300010

Eficiência de índices fenotípicos de comprimento de raiz seminal na avaliação de plantas individuais de milho quanto à tolerância ao alumínio
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Martins,Paulo Roberto; Parentoni,Sidney Netto; Lopes,Maurício Antônio; Paiva,Edilson.
O objetivo deste trabalho foi testar a eficiência do comprimento relativo de raiz seminal (CRRS) e do comprimento líquido de raiz seminal (CLRS) como indicadores fenotípicos quanto à tolerância ao alumínio na avaliação de plantas individuais de milho. Plântulas de genótipos tolerantes e suscetíveis ao Al foram submetidas a solução nutritiva contendo nível tóxico deste elemento, por um período de sete dias, após o qual, determinaram-se os valores de CRRS e CLRS. Os resultados obtidos quando se utilizaram valores médios para CRRS e CLRS mostraram que ambos os índices foram capazes de discriminar com eficiência os materiais tolerantes dos suscetíveis. Entretanto, quando foram utilizados os valores de CRRS e CLRS obtidos a partir de plantas individuais,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solução nutritiva; Toxidez de alumínio; Avaliação de plântulas.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001000017

Marcadores moleculares microssatélites na avaliação de sementes de soja com variação na coloração do hilo
Provedor de dados: Revista Brasileira de Sementes
Autores: Rabel,Marcelo; Vieira,Elisa Serra Negra; Lana,Ubiraci Gomes de Paula; Paiva,Edilson; Sehnem,Margarete Aparecida Sella; Schuster,Ivan.
Marcadores microssatélite têm sido utilizados na determinação da pureza genética em programas de melhoramento e de produção de sementes, visando garantir a qualidade genética das sementes. Objetivou-se neste trabalho demonstrar, com auxílio de marcadores moleculares microssatélites, que variações na coloração do hilo em sementes de soja, muitas vezes não constituem variação genética. Foram avaliadas sementes de soja da cultivar CD 222 que apresenta sementes com hilo preto, e de duas linhagens (CD 02RV-8444 e CD 01RV-7618), as quais apresentam sementes com hilo marrom. As sementes com variações na coloração e tonalidade do hilo encontradas em cada genótipo foram separadas visualmente em grupos. O DNA de cada semente foi extraído e analisado com marcadores...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Cor do hilo; Pureza genética; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222010000200002

Effects of high temperature on survival, symbiotic performance and genomic modifications of bean nodulating Rhizobium strains
Provedor de dados: Revista de Microbiologia
Autores: Pinto,Patrícia P.; Raposeiras,Ruy; Macedo,Andrea M.; Seldin,Lucy; Paiva,Edilson; Sá,Nadja M.H.
High temperatures can affect the survival, establishment and symbiotic properties of Rhizobium strains. Bean nodulating Rhizobium strains are considered particularly sensitive because on this strains genetic recombinations and/or deletions occur frequently, thus compromising the use of these bacteria as inoculants. In this study R. tropici and R. leguminosarum bv. phaseoli strains isolated from Cerrado soils were exposed to thermal stress and the strains’ growth, survival and symbiotic relationships as well as alterations in their genotypic and phenotypic characteristics were analyzed. After successive thermal shocks at 45ºC for four hours, survival capacity appeared to be strain-specific, independent of thermo-tolerance and was more apparent in R. tropici...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bean nodulating Rhizobium strains; High temperatures.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400012

Identificação através de PCR dos genes CryI de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
Provedor de dados: Anais da Sociedade Entomológica do Brasil
Autores: Valicente,Fernando H.; Barreto,Marliton R.; Vasconcelos,Maria J. V. de; Figueiredo,José E.F. de; Paiva,Edilson.
Fall armyworm, Spodoptera frugiperda (J. E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to Identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Bioassays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryI general primers and their proteins were analised by SDS-PAGE eletrophoresis. All strains present positive results for cryI genes. To further characterize these strains, specific cryI primers were employed. PCR technique showed that some strains harbour the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; CryI genes; Biocontrol; Fall armyworm.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80592000000100018

Insecticidal activity of culture supernatants from Bacillus thuringiensis Berliner strains against Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) larvae
Provedor de dados: Anais da Sociedade Entomológica do Brasil
Autores: Barreto,Marliton R.; Loguercio,Leandro L.; Valicente,Fernando H.; Paiva,Edilson.
Novel vegetative insecticidal proteins (Vips) identified in the supernatant of Bacillus thuringiensis (B.t.) cultures have shown to provide adequate control over a wide spectrum of economically important crop pests. To evaluate the potential applicability of these proteins against fall armyworm (Spodoptera frugiperda Smith) larvae, the most important insect pest for tropical maize, the characteristics and mortality effects of culture supernatants from five B.t. strains were investigated. Striking differences among strains were detected, not only in terms of efficiency in killing the insect, but also regarding to mortality effects of heated and non-heated supernatants, which were used to distinguish the heat-sensitive protein-derived insecticidal fraction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Biological control; Fall armyworm; Vegetative insecticidal proteins; Vip genes.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000400010

Effect of ABA and GA3 on protein mobilization in embryos and cotyledons of angico [Anadenanthera peregrina (L.) speg] seeds during germination
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Barduche,Douglas; Paiva,Renato; Lopes,Mauricio A.; Paiva,Edilson.
In this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABA; GA3; Germination; Protein pattern; Protease activity; Tropical woody plant.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200002

A search for RFLP markers to identify genes for aluminum tolerance in maize
Provedor de dados: Brazilian Journal of Genetics
Autores: Torres,Giovana A.; Parentoni,Sidney N.; Lopes,Maurício A.; Paiva,Edilson.
The objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregant analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotypic measure of tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals....
Tipo: Info:eu-repo/semantics/article
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300017

Random amplified polymorphic DNA analysis of effective Rhizobium sp. associated with beans cultivated in brazilian cerrado soils
Provedor de dados: Brazilian Journal of Microbiology
Autores: Oliveira,Isnia Aparecida de; Vasconcellos,Maria José; Seldin,Lucy; Paiva,Edilson; Vargas,Milton Alexandre; Sá,Nadja Maria Horta de.
Efficient bean nodulating Rhizobium strains, isolated from different Brazilian cerrado soils, were characterized by RAPD. This study showed great genetic heterogeneity among R. tropici and R. leguminosarum bv. phaseoli strains and allowed the constitution of genetic clusters, besides indicating the most suitable primers for this characterization. The groups of genetically distinct strains can be used in competitiveness studies to select appropriate Rhizobium strains for bean inoculation in cerrado soils.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizobium tropici; Rhizobium leguminosarum bv. phaseoli; Bean plants; Cerrado soils.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100010

ANALYSES OF GENETIC VARIABILITY IN LENTINULA EDODES THROUGH MYCELIA RESPONSES TO DIFFERENT ABIOTIC CONDITIONS AND RAPD MOLECULAR MARKERS
Provedor de dados: Brazilian Journal of Microbiology
Autores: Maki,Cristina Sayuri; Teixeira,Flavia França; Paiva,Edilson; Paccola-Meirelles,Luzia Doretto.
The growth of thirty-four Lentinula edodes strains submitted to different mycelial cultivation conditions (pH and temperature) was evaluated and strain variability was assessed by RAPD molecular markers. The growth at three pH values (5, 6 and 7) and four different temperatures (16, 25, 28 and 37ºC) was measured using the in vitro mycelial development rate and water retention as parameters. Mycelial cultivation was successful at all pH tested, while the ideal temperature for mycelial cultivation ranged between 25 and 28ºC. The water content was lower in strains grown at 37ºC. Among 20 OPA primers (Operon Technologies, Inc.) used for the RAPD analyses, seventeen presented good polymorphism (OPA01 to OPA05, OPA07 to OPA14, OPA17 to OPA20). The clustering...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edible mushroom; RAPD; Mycelial growth.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300002

Characterization of rhizobia that nodulate Arachis pintoi by RAPD analysis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Pinto,Patrícia Pereira; Paiva,Edilson; Purcino,Hortência; Passos,Raul Vinícius Magalhães; Sá,Nadja Maria Horta.
The genetic relationships of 85 Arachis pintoi nodulating Rhizobium strains were determined using the random amplified polymorphic DNA (RAPD) methods. The analysis included 75 strains isolated from Cerrado soils and 10 other ones of different origins. The results indicated that there is a high level of similarity between these strains and that geographic distribution may affect their phylogenetic relationship. In addition, the results allowed the selection of the most suitable primers for characterisation of these Rhizobium strains which will be useful for implementation of competitiveness studies in Cerrado soils.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizobium; Arachis pintoi; Cerrado soils.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200009

Variability of isolated colonies in bean nodulating Rhizobium strains before and after exposure to high temperature
Provedor de dados: Brazilian Journal of Microbiology
Autores: Raposeiras,Rui; Pinto,Patrícia P.; Passos,Raul V.M.; Seldin,Lucy; Paiva,Edilson; Scotti,M. Rita; Sá,Nadja M.H.
Irregular response to bean plants to Rhizobium inoculation has been attributed to among other factors, low competitive ability, low N2 fixation efficiency and genetic instability of the symbiont. This genetic instability caused by high rates of genomic rearrangements and/or plasmid deletions can be accentuated by high temperatures. This fact may limit the utilization of these strains as inoculants, especially in tropical soils. In this study, the variability of isolated colonies derived from effective R. leguminosarum bv. phaseoli (SLP1.3 and BR 10.026) and R tropici (SLA2.2 and BR322) strains was evaluated before and after exposure to high temperatures (four consecutive thermal shocks at 45ºC). This evaluation involved plant dry matter analysis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizobium tropici; Rhizobium leguminosarum bv. phaseoli; Bean plants; High temperature; Cerrado soils.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200010

Padrões eletroforéticos das proteínas de reserva em grãos de sorgo com presença e ausência de tanino
Provedor de dados: Ciência e Agrotecnologia
Autores: Rodrigues,Walter Alvarenga; Magalhães,Paulo César; Paiva,Edilson; Santos,Fredolino Giacomini dos.
Muitos métodos para determinar a presença de taninos são descritos na literatura, mas nenhum deles é universalmente aceito como ideal ou utilizado de forma unânime. Alguns métodos colorimétricos não diferenciam taninos de outros compostos fenólicos, outros utilizam substâncias que não são adequadas como padrão. Métodos que utilizam a capacidade dos taninos de precipitar as proteínas podem causar resultados divergentes devido às diferenças na conformação dessas moléculas. Assim, objetivou-se, neste estudo, identificar a presença de taninos em 10 híbridos de sorgo através da análise de padrões proteicos obtidos por eletroforese. O método colorimétrico Azul da Prússia foi utilizado para quantificar os taninos nas amostras. A precipitação das proteínas pelos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compostos fenólicos; Método Azul da Prússia; Precipitação de proteínas.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000700047

Indução e análise bioquímica de calos obtidos de segmentos foliares de Coffea arabica L., Cultivar Rubi
Provedor de dados: Ciência e Agrotecnologia
Autores: Santos,Cíntia Guimarães dos; Paiva,Renato; Paiva,Patrícia Duarte de Oliveira; Paiva,Edilson.
Conduziu-se este trabalho com o objetivo de estudar o efeito de 2,4-D e BAP na indução da formação de calos originados de segmentos foliares de plântulas de Coffea arabica L., cv Rubi, obtidas in vitro, a partir da cultura de embriões, determinar sua curva de crescimento e analisar bioquimicamente, mediante determinação dos teores de proteínas totais, açúcares redutores e padrão proteíco. Segmentos foliares inoculados em meio desprovido de reguladores ou na presença de BAP em condições isoladas não apresentaram formação de calos. A produção máxima de calos é obtida utilizando-se 1,0 mg L-1 de 2,4-D. A curva de crescimento de calos apresenta crescimento tipo sigmóide, com três fases distintas: lag (entre 0 - 42 dias de inoculação), exponencial (42 - 77...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Indução de calos; Padrão protéico.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000300011