Consulta: creatorFacets:"Penha Filho,Rafael Antonio Casarin"
Registros recuperados: 3
Data/hora: 03/06/2024 04:32:21

Requirement for cobalamin by Salmonella enterica serovars Typhimurium, Pullorum, Gallinarum and Enteritidis during infection in chickens
Provedor de dados: Brazilian Journal of Microbiology
Autores: Paiva,Jacqueline Boldrin de; Penha Filho,Rafael Antonio Casarin; Berchieri Junior,Angelo; Lemos,Manoel Victor Franco.
Salmonella enterica serovar Typhimurium synthesizes cobalamin (vitamin B12) only during anaerobiosis. Two percent of the S. Typhimurium genome is devoted to the synthesis and uptake of vitamin B12 and to B12-dependent reactions. To understand the requirement for cobalamin synthesis better, we constructed mutants of Salmonella serovars Enteritidis and Pullorum that are double-defective in cobalamin biosynthesis (ΔcobSΔcbiA). We compared the virulence of these mutants to that of their respective wild type strains and found no impairment in their ability to cause disease in chickens. We then assessed B12 production in these mutants and their respective wild type strains, as well as in S. Typhimurium ΔcobSΔcbiA, Salmonella Gallinarum ΔcobSΔcbiA, and their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Cobalamin; Gene deletion; Chickens.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400024

A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens
Provedor de dados: Brazilian Journal of Microbiology
Autores: Paiva,Jacqueline Boldrin de; Penha Filho,Rafael Antonio Casarin; Arguello,Yuli Melisa Sierra; Berchieri Junior,Ângelo; Lemos,Manuel Victor Franco; Barrow,Paul A.
Salmonella enterica serovar Gallinarum (SG) is a fowl typhoid agent in chickens and is a severe disease with worldwide economic impact as its mortality may reach up to 80%. It is one of a small group of serovars that typically produces typhoid-like infections in a narrow range of host species and which therefore represents a good model for human typhoid. The survival mechanisms are not considered to be virulent mechanisms but are essential for the life of the bacterium. Mutants of Salmonella Gallinarum containing defective genes, related to cobalamin biosynthesis and which Salmonella spp. has to be produced to survive when it is in an anaerobic environment, were produced in this study. Salmonella Gallinarum is an intracellular parasite. Therefore, this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella Gallinarum; Cobalamin biosynthesis; Attenuated strain; CbiA and cobS genes; CbiA; CobS.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300012

Antimicrobial susceptibility of Salmonella Gallinarum and Salmonella Pullorum isolated from ill poultry in Brazil
Provedor de dados: Ciência Rural
Autores: Penha Filho,Rafael Antonio Casarin; Ferreira,Joseane Cristina; Kanashiro,Ana Maria Iba; Darini,Ana Lúcia da Costa; Berchieri Junior,Angelo.
ABSTRACT: Salmonella Gallinarum (S. Gallinarum) and Salmonella Pullorum (S. Pullorum) are poultry host-specific, agents of fowl typhoid and pullorum disease, respectively. These biovars cause septicemic infections, resulting in high mortality. Outbreaks are frequently reported worldwide, causing losses due to the elimination of infected flocks and treatments. The use of antimicrobial agents is frequent in poultry farms to prevent or treat gastrointestinal infections. In the present research it was evaluated the antimicrobial susceptibility of 50 S. Gallinarum and S. Pullorum isolates, from outbreaks that occurred between 1987 to 1991 and 2006 to 2013. The comparison of the susceptibility profiles showed that all isolates were susceptible to β-lactams. All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quinolones; Chicken; Fowl typhoid; Antimicrobial susceptibility; Salmonella.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300513