Consulta: creatorFacets:"Pereira,Luciane Aparecida"
Registros recuperados: 3
Data/hora: 02/06/2024 06:29:58

Comparison of immunoperoxidase and immunofluorescence assays for pp65 cytomegalovirus antigen in immunocompromised patients
Provedor de dados: 55
Autores: Debur,Maria do Carmo; Vidal,Luine R. Rosele; Nogueira,Meri Bordignon; Pereira,Luciane Aparecida; Takahashi,Gislene R.A.; Rotta,Indianara; Almeida,Sérgio Monteiro de; Raboni,Sonia Mara.
We compared the pp65 antigen detection by an in house method (immunoperoxidase assay) and by a commercial kit (immunofluorescence assay) available for cytomegalovirus infection diagnosis in immunocompromised patients. Sixty-four blood samples were analyzed in duplicate for both techniques. Eight-six percent of the samples had concordant qualitative results. The discordant results occurred more frequently in samples with low quantity of positive cells. There were no significant differences with qualitative and quantitative results of the methods.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunoperoxidase; Immunofluorescence; Cytomegalovirus; Immunocompromised patients; Bone marrow transplantation; Solid organ transplant.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200014

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization
Provedor de dados: 55
Autores: Pereira,Luciane Aparecida; Raboni,Sonia Mara; Nogueira,Meri B; Vidal,Luine R; Almeida,Sergio Monteiro de; Debur,Maria C; Cruz,Cristina.
BACKGROUND AND OBJECTIVES: Rotavirus (RV) is the main etiological agent of diarrhea in childhood; its laboratory diagnosis is crucial to guide the clinical management and prevention of its spread. RV immunization was introduced in Brazilian 6-month-old children in 2006. The present study was aimed to evaluate three methodologies used for human RV detection in stool samples obtained from patients hospitalized due to gastroenteritis in a teaching hospital and report the impact of RV immunization in hospitalization by diarrhea. METHODS: 293 stool samples collected in the 2001-2008 period were analyzed by enzyme immunoassay (EIA), latex agglutination (LA) and polyacrylamide gel electrophoresis (PAGE). RESULTS: Rotavirus was detected in 34.8% of samples by LA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Diarrhea; Immunoenzyme techniques; Latex fixation tests; Electrophoresis; Polyacrylamide gel.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300006

Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients
Provedor de dados: 55
Autores: Ferreira,Carla Elizabeth de Oliveira; Raboni,Sonia Mara; Pereira,Luciane Aparecida; Nogueira,Meri Bordignon; Vidal,Luine Rosele Renaud; Almeida,Sérgio Monteiro.
BACKGROUND: Acute gastroenteritis (AGE) is a common disorder that affects children worldwide. It is usually caused by viral agents, including rotavirus, enteric adenovirus, norovirus, and astrovirus groups. Currently, there are few reports about co-infection among these viruses, mainly in Brazil. METHODS: This is a retrospective study in which 84 rotavirus-positive samples from hospitalized patients at a teaching hospital in Southern Brazil, collected in the 2001-2010 period, were analyzed by polymerase chain reaction (PCR) and reverse transcription - polymerase chain reaction (RT-PCR), for the investigation of enteric adenovirus, astrovirus, and norovirus. RESULTS: In total, 12 of the 84 (14%) samples were positive to enteric adenovirus or norovirus....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-infection; Viral gastroenteritis; Oiarrhea; Rotavirus A; Norovirus; Enteric adenovirus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300009