Consulta: creatorFacets:"Perez Garcia, Maria Concepcion"
Registros recuperados: 3
Data/hora: 05/06/2024 06:44:49

Karyotypic diversification in Mytilus mussels (Bivalvia: Mytilidae) inferred from chromosomal mapping of rRNA and histone gene clusters
Provedor de dados: 5
Autores: Perez Garcia, Maria Concepcion; Moran, Paloma; Pasantes, Juan J.
Background: Mussels of the genus Mytilus present morphologically similar karyotypes that are presumably conserved. The absence of chromosome painting probes in bivalves makes difficult verifying this hypothesis. In this context, we comparatively mapped ribosomal RNA and histone gene families on the chromosomes of Mytilus edulis, M. galloprovincialis, M. trossulus and M. californianus by fluorescent in situ hybridization (FISH). Results: Major rRNA, core and linker histone gene clusters mapped to different chromosome pairs in the four taxa. In contrast, minor rRNA gene clusters showed a different behavior. In all Mytilus two of the 5S rDNA clusters mapped to the same chromosome pair and one of them showed overlapping signals with those corresponding to one...
Tipo: Text
Palavras-chave: Mytilus; Chromosome; Fluorescent in situ hybridization; Histone genes; Ribosomal RNA genes; Fibre-FISH.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00204/31500/29906.pdf

Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas
Provedor de dados: 5
Autores: Perez Garcia, Maria Concepcion; Rouxel, Julien; Akcha, Farida.
In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay. Next, NOR sensitivity was analysed by mapping major rDNA repeat unit by fluorescence in situ hybridisation (FISH) on the same comet slides. Exposure of hemocytes to 100 μM of hydrogen peroxide induced a significant increase in both DNA damage and number of FISH-signals of major ribosomal genes versus the control. Moreover, a significant positive correlation was shown between DNA damage as measured by the comet assay (percentage of DNA in comet tail) and the...
Tipo: Text
Palavras-chave: Crassostrea gigas; Hydrogen peroxide; Comet assay; Comet-FISH; Genotoxicity.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00250/36157/34712.pdf

Cytogenetic evidences of genome rearrangement and differential epigenetic chromatin modification in the sea lamprey (Petromyzon marinus)
Provedor de dados: 5
Autores: Covelo-soto, Lara; Moran, Paloma; Pasantes, Juan J.; Perez Garcia, Maria Concepcion.
This work explores both the chromatin loss and the differential genome methylation in the sea lamprey (Petromyzon marinus) from a molecular cytogenetic point of view. Fluorescent in situ hybridization experiments on meiotic bivalents and mitotic chromosomes corroborate the chromatin loss previously observed during the development of the sea lamprey and demonstrate that the elimination affects not only to Germ1 sequences but also to the rpt200 satellite DNA and most part of the major ribosomal DNA present on the germinal line. 5-Methylcytosine immunolocation revealed that the GC-rich heterochromatin is highly methylated in the germ line but significantly less in somatic chromosomes. These findings not only support previous observations about genome...
Tipo: Text
Palavras-chave: Sea lamprey; Chromatin loss; Genome rearrangements; DNA methylation.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00247/35811/34327.pdf