Consulta: creatorFacets:"Pileggi,Marcos"
Registros recuperados: 6
Data/hora: 14/06/2024 12:56:54

Rapid PCR-based assay for Sclerotinia sclerotiorum detection on soybean seeds
Provedor de dados: Scientia Agricola
Autores: Grabicoski,Edilaine Mauricia Gelinski; Jaccoud Filho,David de Souza; Pileggi,Marcos; Henneberg,Luciane; Pierre,Marcelo Luiz Cunha; Vrisman,Cláudio Mauricio; Dabul,Audrei Nisio Gebieluca.
Caused by Sclerotinia sclerotiorum, white mold is an important seed-transmitted disease of soybean (Glycine max). Incubation-based methods available for the detection and quantification of seed-borne inoculum such as the blotter test, paper roll and Neon-S assay are time-consuming, laborious, and not always sensitive. In this study, we developed and evaluated a molecular assay for the detection of S. sclerotiorum in soybean seeds using a species-specific PCR (polymerase chain reaction) primer set and seed soaking (without DNA extraction) for up to 72 h. The PCR products were amplified in all the samples infected with the pathogen, but not in the other samples of plant material or the other seed-borne fungi DNA. The minimum amount of DNA detected was 10 pg,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: White mold; Dissemination; Soaking seeds; Diagnostic.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100069

Selection of microorganisms degrading S-Metolachlor herbicide
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Martins,Paula Fabiane; Martinez,Camila Ortiz; Carvalho,Giselle de; Carneiro,Paulo Irajara Borba; Azevedo,Ricardo Antunes; Pileggi,Sônia Alvim Veiga; Melo,Itamar Soares de; Pileggi,Marcos.
The aim of this work was to study herbicide degradation through selected microorganisms from humus and soil subjected to different plantation systems. The following bacterial species were identified: Klebsiella pneumoniae pneumoniae GC s.B strain 1, Pseudomonas alcaligenes, Enterobacter aerogenes GC s.A and Klebsiella pneumoniae pneumoniae GC s.B strain 2. Growth studies yet suggested the possibility of a very long lag phase. Although, culture with the herbicide presented biofilm formation and there were color changes in the herbicide that could have interfered with the espectrophotometry readings. After 5 days of incubation at 35ºC, the difference in the concentration of herbicide was 14.42% on average and after 10 days, 35.01%.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioremediation; Biodegradation; S-Metolachlor; Herbicide.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100019

An improved method for transformation of lettuce by Agrobacterium tumefaciens with a gene that confers freezing resistance
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Pileggi,Marcos; Pereira,Albanin Aparecida Mielniczki; Silva,Joandrei dos Santos; Pileggi,Sônia Alvim Veiga; Verma,Desh Pal S.
An efficient method for constructing transgenic lettuce cultivars by Agrobacterium tumefaciens was described by Torres et al., 1993. In the present work, an improvement of the above procedure is described and applied to transform the cultivar Grand Rapids with a mutated P5CS gene. The major modifications were concerned with turning more practical the transformation and regeneration protocols. Also we tried to improve transformation steps by increasing injured area in explants and prolonging co-cultivation with Agrobacteria (in larger concentration). A more significant selective pressure was used against non-transformed plants and bacteria. In these work we were concerned to obtain T1 and T2 seeds. The P5CS gene codes for a delta¹-pyrroline-5-carboxylate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress resistance; Cold resistance; Transgenic; Lettuce; Proline.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200013

Selective agent and A. tumefaciens overgrowth-control antibiotics in Eucalyptus camaldulensis Cotiledonary culture
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Quisen,Regina; Oliveira,Yohana de; Pileggi,Marcos; Cuquel,Francine; Quoirin,Marguerite.
The objectives of the present work were to establish the minimal lethal dose of the selective agent to determine the type and concentration of appropriate antibiotics for the elimination of Agrobacterium tumefaciens inoculated explants, without interfering with the regenerative potential of the E. camaldulensis cotyledonary explants. Non-transformed explants were cultivated in medium supplemented with kanamycin. The results showed that the antibiotic was suitable for the selection of transformed cells in the concentration of 9 mg L-1 as it inhibited the growth of non-transformed cells. Cotyledons infected with A. tumefaciens were cultivated in MS N/2 medium supplemented with BAP, ANA, Km and cefotaxime or AugmentinÒ . The highest average of regenerated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphogenesis; Kanamycin; Cefotaxime; Augmentin; Agrobacterium tumefaciens; Eucalypt.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600020

Degradation of 2,4-D herbicide by microorganisms isolated from Brazilian contaminated soil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Silva,Tatiane M.; Stets,Maria I.; Mazzetto,André M.; Andrade,Fabiana D.; Pileggi,Sônia A. V.; Fávero,Paulo R.; Cantú,Marcelo D.; Carrilho,Emanuel; Carneiro,Paulo I.B.; Pileggi,Marcos.
The aim of this work was to isolate microorganisms from Brazilian soil contaminated with 2,4-D herbicide, and analyze the efficiency for 2,4D degradation, using high-performance liquid chromatography (HPLC). Serratia marcescens and Penicillium sp had never been reported as able to degrade 2,4-D. The isolated strains represent a great potential for bioremediation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioremediation Biodegradation 2; 4-dichlorophenoxyacetic acid.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300026

Selection of endophytic fungi from comfrey (Symphytum officinale L.) for in vitro biological control of the phytopathogen Sclerotinia sclerotiorum (Lib.)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Rocha,Rafaeli; Luz,Daniela Eleutério da; Engels,Cibelle; Pileggi,Sônia Alvim Veiga; Jaccoud Filho,David de Souza; Matiello,Rodrigo Rodrigues; Pileggi,Marcos.
Biological control consists of using one organism to attack another that may cause economic damage to crops. Integrated Pest Management (IPM) is a very common strategy. The white mold produced by Sclerotiniasclerotiorum (Lib.) causes considerable damage to bean crops. This fungus is a soil inhabitant, the symptoms of which are characterized by water-soaked lesions covered by a white cottony fungal growth on the soil surface and/or the host plant. Possible biological control agents taken from plants are being investigated as phytopathogen inhibitors. These are endophytic microorganisms that inhabit the intercellular spaces of vegetal tissues and are often responsible for antimicrobial production. The objective of the present study was to select endophytic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Sclerotinia sclerotiorum (Lib.); Endophytic strain; Symphytum officinale L.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100011