Consulta: creatorFacets:"Presti,Flavia T."
Registros recuperados: 2
Data/hora: 29/05/2024 03:01:41

Comparative analysis of microsatellite variability in five macaw species (Psittaciformes, Psittacidae): application for conservation
Provedor de dados: 74
Autores: Presti,Flavia T.; Oliveira-Marques,Adriana R.; Caparroz,Renato; Biondo,Cibele; Miyaki,Cristina Y.
Cross-amplification was tested and variability in microsatellite primers (designed for Neotropical parrots) compared, in five macaw species, viz., three endangered blue macaws (Cyanopsitta spixii [extinct in the wild], Anodorhynchus leari [endangered] and Anodorhynchus hyacinthinus [vulnerable]), and two unthreatened red macaws (Ara chloropterus and Ara macao). Among the primers tested, 84.6% successfully amplified products in C. spixii, 83.3% in A. leari, 76.4% in A. hyacinthinus, 78.6% in A. chloropterus and 71.4% in A. macao. The mean expected heterozygosity estimated for each species, and based on loci analyzed in all the five, ranged from 0.33 (A. hyacinthinus) to 0.85 (A. macao). As expected, the results revealed lower levels of genetic variability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Cross-amplification; Macaw; Microsatellite; Psittaciformes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200030

Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus)
Provedor de dados: 74
Autores: Presti,Flavia T.; Meyer,Janaína; Antas,Paulo T.Z.; Guedes,Neiva M.R.; Miyaki,Cristina Y.
Molted feather sampling is a useful tool for genetic analyses of endangered species, but it is often very laborious due to the low quality and quantity of the DNA obtained. In the present study we show the parts of feathers that resulted in better yield of DNA. In descending order these were: blood clot outside the umbilicus, umbilicus (without blood clot), tip, inner membrane, and small calamus. Compared to DNA extracted from blood samples, DNA extracted from feathers produced microsatellite alleles of poorer quality and had to be processed immediately after extraction. As expected due to the level of DNA degradation, molecular sexing protocols that result in shorter PCR products were more efficient.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molted feathers; Blood sample; DNA; PCR product; Parrot.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100019