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Data/hora: 17/06/2024 16:29:16

Cryopreservation of mouse morulae through different methods: slow-freezing, vitrification and quick-freezing
Provedor de dados: 62
Autores: MELLO,Marco Roberto Bourg de; QUEIROZ,Vinícius Seixas; LIMA,Alessandra Sobreira de; TAVARES,Liliam Mara Trevisam; ASSUMPÇÃO,Mayra Elena Ortiz D'Avila; WHEELER,Mathew B.; VISINTIN,José Antonio.
The in vitro and in vivo development of mouse morulae after cryopreservation through different methods was examined. The slow-freezing involved an equilibration in 1.5M ethylene glycol (EG) and cooled at 0.5; 0.7; 1.0 or 1.2ºC/minute. The vitrification involved a 3 minutes equilibration in 20% EG and 60 seconds in solution containing 40% EG, 18% ficoll and 10.26% sucrose. The quick-freezing involved an equilibration in 3M EG + 0.3M sucrose for 5 minutes and 2 minutes in nitrogen vapor. In all three methods the straws were thawed in air for 10 seconds and in water at 25ºC for 20 seconds and the embryos cultured in vitro for 72 hours to estimate blastocyst rate. To assess viability in vivo, frozen morulae as well as fresh embryos were transferred into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Embryo; Mouse.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000400003