Consulta: creatorFacets:"Qiu,Shanlian"
Registros recuperados: 3
Data/hora: 09/06/2024 02:10:53

Abundance and diversity of ammonia-oxidizing bacteria in relation to ammonium in a Chinese shallow eutrophic urban lake
Provedor de dados: 58
Autores: Qiu,Shanlian; Chen,Guoyuan; Zhou,Yiyong.
The measures of most-probable-number and restriction fragment length polymorphism analysis were used to analyze the abundance and diversity of ammonia-oxidizing bacteria in sediment of a Chinese shallow eutrophic urban lake (Lake Yuehu). Among the 5 sampling sites, ammonia concentration in interstitial water was positively proportional not only to the content of organic matter, but also to ammonia-oxidizing bacteria numbers (at a magnitude of 10(5) cells g-1 dry weight) in sediment significantly. Furthermore, the diversity of ammonia-oxidizing bacteria were determined by means of PCR primers targeting the amoA gene with five gene libraries created and restriction pattern analysis. The 13 restriction patterns were recorded with 4 ones being common among all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammonia-oxidizing bacteria; Abundance; AmoA; Diversity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100031

Effects of open drainage ditch design on bacterial and fungal communities of cold waterlogged paddy soils
Provedor de dados: 58
Autores: Qiu,Shanlian; Wang,MK; Wang,Fei; Chen,Jichen; Li,Xiaoyan; Li,Qinghua; Lin,Cheng; Lin,Xinjian.
A field experiment established in 1980 was conducted to evaluate the effects of open drainage ditch applied for water removal on bacterial and fungal communities of cold waterlogged paddy soils in 2011. In this experiment, traditional plate counting and temperature gradient gel electrophoresis were employed to characterize the abundance and diversity of soil bacterial and fungal communities. Four different distances from the open drainage ditch, 5, 15, 25 and 75 m with different degrees of drainage were designed for this study. Maximum populations of culturable aerobic bacteria and fungi were at 15-m distance while minimum populations were at 75-m distance. Significant differences (p &lt; 0.05) in fungal populations were observed at all distances from open...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abundance; Bacterial and fungal communities; Diversity; Open drainage ditch.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300050

A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
Provedor de dados: 58
Autores: Qiu,Shanlian; Chen,Jichen; Lin,Si; Lin,Xinjian.
Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Backed gel; DNA; Silver staining.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200029