Consulta: creatorFacets:"Queiroz,D.M.M."
Registros recuperados: 4
Data/hora: 03/06/2024 03:34:34

Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rocha,G.A.; Oliveira,A.M.R.; Queiroz,D.M.M.; Mendes,E.N.; Moura,S.B.; Oliveira,C.A.; Ferrari,T.C.A.
We evaluated the accuracy of a 2nd generation ELISA to detect Helicobacter pylori infection in adults from a developing country in view of variations in sensitivity and specificity reported for different populations. We studied 97 non-consecutive patients who underwent endoscopy for evaluation of dispeptic symptoms. The presence of H. pylori was determined in antral biopsy specimens by culture, by the preformed urease test and in carbolfuchsin-stained smears. Patients were considered to be H. pylori positive if at least two of the three tests presented a positive result or if the culture was positive, and negative if the three tests were negative. Sixty-five adults (31 with peptic ulcer) were H. pylori positive and 32 adults were H. pylori negative....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Serodiagnosis; ELISA.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000005

The role of IFN-gamma and IL-4 in gastric mucosa inflammation associated with Helicobacter heilmannii type 1 infection
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cinque,S.M.S.; Rocha,G.A.; Correa-Oliveira,R.; Soares,T.F.; Moura,S.B.; Rocha,A.M.C.; Nogueira,A.M.M.F.; Cabral,M.M.D.A.; Vieira,L.Q.; Martins-Filho,O.A.; Queiroz,D.M.M.
Although Helicobacter heilmannii infection is less common than H. pylori infection in humans, it is considered to be of medical importance because of its association with gastritis, gastric ulcer, carcinoma, and mucosa-associated lymphoid tissue lymphoma of the stomach. However, there have been no studies evaluating the role of the Th cell response in H. heilmannii gastric infection. We evaluated the participation of pro-inflammatory and anti-inflammatory cytokines, IFN-gamma and IL-4, in H. heilmannii gastric infection in genetically IFN-gamma- or IL-4-deficient mice. The serum IFN-gamma and IL-4 concentrations were determined by ELISA. The gastric polymorphonuclear infiltrate was higher (P = 0.007) in H. heilmannii-positive than in H. heilmannii-negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter heilmannii; Gastric mucosa inflammation; IFN-gamma-/- mice; IL-4-/- mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200012

Hepatic changes in mice chronically infected with Helicobacter trogontum
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Moura,S.B.; Queiroz,D.M.M.; Rocha,G.A.; Comunian,L.B.; Cara,D.C.
We infected NIH germ-free female mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the lesions it induced in the liver of this host. Fifteen mice were challenged with a single dose of H. trogontum (test group) and killed 6, 12 and 18 months after inoculation (5 animals/group). Nine animals were challenged with 0.85% saline alone (control group) and killed at the same times. Fragments from the liver, cecum and colon were obtained for microbiologic and histologic examination. Stool samples were also collected. H. trogontum was detected in the cecum, colon and/or stool samples of all test mice. As expected, the bacterium was not isolated from any specimen obtained from the control animals. On the other...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Helicobacter; Helicobacter trogontum; Animal model; Liver; Mice.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900010

Mouse inoculation for the detection of non-cultivable gastric tightly spiralled bacteria
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Mendes,E.N.; Queiroz,D.M.M.; Moura,S.B.; Rocha,G.A.
In the present study we compared the inoculation of swine gastric mucus into the stomach of mice, the urease test and carbolfuchsin-stained smears for the diagnosis of the infection with "Gastrospirillum suis" ("Helicobacter heilmannii" type 1), an uncultivated tightly spiralled gastric bacterium. Fragments obtained from the antral and oxyntic mucosa of the stomach of 50 slaughtered pigs were used for urease test, for carbolfuchsin-stained smears and for obtaining scrapings of mucus for mouse inoculation. The mice were killed by spinal dislocation 10 days after inoculation and fragments of the antral and oxyntic mucosa were used for spiral bacterium identification (urease test and carbolfuchsin-stained smears). Among the methods employed for the diagnosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter; Helicobacter heilmannii; Gastrospirillum/infection diagnosis; Gastric spiral bacteria; Helicobacter infection diagnosis; Mouse inoculation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300007