Consulta: creatorFacets:"Queiroz,Paulo Roberto"
Registros recuperados: 3
Data/hora: 02/06/2024 02:16:20

Survival in soil and detection of co-transformed Trichoderma harzianum by nested PCR
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Queiroz,Paulo Roberto; Valadares-Inglis,Maria Cléria; Inglis,Peter Ward.
The objective of this work was to evaluate the survival of two Trichoderma harzianum co-transformants, TE 10 and TE 41, carrying genes for green fluorescent protein (egfp) and for resistance to benomyl, during four weeks in a contained soil microcosm. Selective culture media were used to detect viable fungal material, whose identity was confirmed by the observation of the fluorescent phenotype by direct epifluorence microscopy. PCR using two nested primer pairs specific to the egfp gene was also used to detect the transformed fungi. Although it was not possible to reliably detect the egfp gene directly from soil extracts, an enrichment step involving selective culture of soil samples in liquid medium prior to DNA extraction enabled the consistent detection...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Biological control; Transformation; Egfp; Benomyl resistance.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000400015

Identification of the B, Q, and native Brazilian biotypes of the Bemisia tabaci species complex using Scar markers
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Queiroz,Paulo Roberto; Martins,Erica Soares; Klautau,Nazaré; Lima,Luzia; Praça,Lilian; Monnerat,Rose Gomes.
Abstract: The objective of this work was to develop sequence-characterized amplified region (Scar) markers to identify the B, Q, and native Brazilian biotypes of the sweet potato whitefly [Bemisia tabaci (Hemiptera: Aleyrodidae)]. Random amplified polymorphic DNA (RAPD) amplification products, exclusive to the B and Brazilian biotypes, were selected after the analysis of 12,000 samples, in order to design a specific Scar primer set. The BT-B1 and BT-B3 Scar markers, used to detect the B biotype, produced PCR fragments of 850 and 582 bp, respectively. The BT-BR1 Scar marker, used to identify the Brazilian biotype, produced a PCR fragment of 700 bp. The Scar markers were tested against the Q biotype, and a flowchart was proposed to indicate the decision...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aleyrodidae; Commodities; Field samples; Molecular marker; Sweet potato whitefly.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500555

Mitochondrial markers to distinguish Spodoptera frugiperda populations associated with corn and cotton crops
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Queiroz,Paulo Roberto; Ramiro,Carolina Almeida; Martins,Érica Soares; Soberón,Mário; Bravo,Alejandra; Monnerat,Rose Gomes.
Abstract: The objective of this work was to analyze the genetic variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) populations collected from corn and cotton crops in Brazil. The samples were analyzed by DNA markers. The dendrogram produced by the 20 RAPD primers evaluated showed a correlation between genetic profile and feeding behavior. The analysis of the mitochondrial ND1 gene allowed identifying the insect populations in both crops, and, in corn, in several geographical regions. The presented strategy allows the identification of Spodoptera frugiperda populations associated with corn and cotton crops.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gossypium hirsutum; Zea mays; Fall armyworm; Haplotypes; ND1 gene; RAPD.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500692