Consulta: creatorFacets:"Quere, Claudie"
Registros recuperados: 42
Data/hora: 01/06/2024 08:41:59

Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana; Milan, Massimo; Guo, Xinmei; Li, Li; Zhang, Guofan; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte.
AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism...
Tipo: Text
Palavras-chave: Marine bivalve; Crassostrea gigas; Hypoxia; AMP-activated protein kinase; Alternative splicing; Adductor muscle.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00119/22998/21397.pdf

Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Huvet, Arnaud; Jeffroy, Fanny; Daniel, Jean-yves; Quere, Claudie; Le Souchu, Pierrick; Van Wormhoudt, A.; Boudry, Pierre; Moal, Jeanne; Samain, Jean-francois.
In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to food availability, whereas the functional role of AMYB was still unknown. To improve knowledge about the regulation of amylase expression in C. gigas and the respective roles of the two genes, we made an assay of amylase expression at mRNA and enzymatic levels in the digestive gland of oysters that had received dietary supplements of starch. After 18 days, a significant increase of translatable mRNA for AMYB was observed, with a correlated increase in Michaelis-Menten constant Km values...
Tipo: Text
Palavras-chave: Alpha-amylase; Crassostrea gigas; Digestive enzyme; Michaelis-Menten constant; Regulation.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00087/19869/17521.pdf

Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc.
In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied 1) changes in spermatozoal mitochondrial activity, that is, mitochondrial membrane potential (MMP), and intracellular ATP content in relation to motion parameters and 2) the involvement of OXPHOS for spermatozoal movement using carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The percentage of motile spermatozoa decreased over a 24 h movement period. MMP increased steadily during the first 9 h of the movement phase and was subsequently maintained at a...
Tipo: Text
Palavras-chave: Crassostrea gigas; Intracellular ATP content; Mitochondrial membrane potential; Oxidative phosphorylation; Sperm motility.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00303/41376/40666.pdf

Techniques for delivery of arachidonic acid to Pacific oyster, Crassostrea gigas, spat
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Seguineau, Catherine; Soudant, P; Moal, Jeanne; Delaporte, Maryse; Miner, Philippe; Quere, Claudie; Samain, Jean-francois.
The present study tested two techniques for dietary supplementation of Crassostrea gigas spat with PUFA, such as arachidonic acid (AA). The first technique consisted of a preliminary enrichment and growth of an algal concentrate (T-ISO, Isochrysis sp.) with AA dissolved in an ethanol solution, the whole culture then being fed to the spat. This enrichment increased the AA weight percentage in T-ISO neutral and polar lipids from 0.6 to 22.4% and from 0.4 to 6.8%, respectively. The second delivery technique was direct addition separately of free AA dissolved in ethanol solution and algal concentrate (T-ISO + AA) to the spat-rearing tank. To test the efficiency of these delivery techniques, oyster spat were supplemented with AA-enriched T-ISO, T-ISO + AA, and...
Tipo: Text
Palavras-chave: Food supply; Arachidonic acid; Spat; Crassostrea gigas; Dietary.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1094.pdf

Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie.
Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters...
Tipo: Text
Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf

Laboratory conditioning modifies properties of gills mitochondria from the Pacific oyster Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Dudognon, Tony; Guderley, Helga; Quere, Claudie; Soudant, Philippe; Racotta, Ilie; Kraffe, Edouard.
Although laboratory experiments allow greater control of environmental conditions than field studies, they have several drawbacks. To analyze physiological responses to forcing environmental variables, experimental conditions should mimic natural conditions as closely as possible. For filter-feeding organisms in particular, diet quality and quantity is one of the environmental parameters that can differ markedly between experimental and field conditions. In the hatchery, Pacific oysters, Crassostrea gigas, commonly show good physiological performance and growth on a mixed algal diet of Tisochrysis lutea, formerly Isochrysis aff. galbana clone Tahiti (T-Iso), and Chaetoceros calcitrans, presumably as it provides a good supply of essential polyunsaturated...
Tipo: Text
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00266/37756/36065.pdf

Facteurs explicatifs de la dynamique spatio-temporelle des mortalités d’huîtres creuses Crassostrea gigas dans la lagune de Thau
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pernet, Fabrice; Lagarde, Franck; Cesmat, Ludovic; Jeannée, Nicolas; Barret, Jean; Le Gall, Patrik; Quere, Claudie; Fiandrino, Annie; Miguet, Marine; Boulet, Hélène; Keck, Nicolas; Roque D'Orbcastel, Emmanuelle.
Cette étude avait pour objectif principal de déterminer les facteurs explicatifs de la dynamique spatio-temporelle des surmortalités d’huîtres creuses dans la lagune de Thau. Les facteurs étudiés étaient : la pathologie (détection d’ADN appartenant à l’Ostreid herpesvirus 1 OsHV-1 et au groupe bactérien Vibrio splendidus), l’état énergétique des huîtres (triglycérides, sucres totaux et acides gras comme marqueurs trophiques), les pratiques d’élevage et l’hydrodynamique. Afin de répondre à ces objectifs, nous avons suivi la mortalité d’un lot d’huîtres sentinelles, âgé de moins d’un an, maintenu indemne de mortalité, dans lequel on ne détectait pas d’ADN appartenant à OsHV-1. Le lot a été déployé mi-mars 2011 sur 106 stations dans la lagune de Thau, où les...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00092/20355/18015.pdf

Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Quere, Claudie; Bruneau, A; Riso, Ricardo; Auffret, Michel; Soudant, Philippe.
Effects of simultaneous exposure of Pacific oyster, Crassostrea gigas, to both a harmful dinoflagellate that produces Paralytic Shellfish Toxins (PST), Alexandrium minutum, and cadmium (Cd) and copper (Cu), were assessed. Oysters were exposed to a mix of Cd-Cu with two different diets (i.e. A. minutum or Tisochrysis lutea) and compared to control oysters fed A. minutum or T. lutea, respectively, without metal addition. Metals and PST accumulations, digestive gland lipid composition, and cellular and biochemical hemolymph variables were measured after 4 days of exposure. Oysters exposed to Cd-Cu accumulated about thirty-six times less PSTs than oysters exposed to A. minutum alone. Exposure to Cd-Cu induced significant changes in neutral lipids (increase in...
Tipo: Text
Palavras-chave: Harmful algae; Metals; Oysters; Toxin accumulation; Physiological effects.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00169/28071/26286.pdf

Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Quere, Claudie; Mingant, Christian; Lebrun, Luc; Ratiskol, Dominique; Miner, Philippe; Cosson, Jacky.
Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed...
Tipo: Text
Palavras-chave: Spermatozoa; CASA; Maturation; Sperm motility; Bivalve; Pecten maximus.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26852/24983.pdf

Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Epelboin, Yanouk; Quere, Claudie; Pernet, Fabrice; Pichereau, Vianney; Corporeau, Charlotte.
Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0.1 mu g/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase alpha (AMPK alpha), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE...
Tipo: Text
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00284/39490/44227.pdf

Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Da Costa, Fiz; Le Grand, Fabienne; Quere, Claudie; Bougaran, Gael; Cadoret, Jean-paul; Robert, Rene; Soudant, Philippe.
Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete and N-reduced conditions to determine the effects of N supply in culture medium and growth phase on microalgal physiology and biochemistry. These two T. lutea strains were compared in terms of growth, morphology, associated free-living bacterial community, viability, intracellular lipid content (as measured by Bodipy staining), chlorophyll autofluorescence, and biochemical composition, with a focus on lipid class and fatty acid compositions. The standard strain (T) reached higher cell counts regardless of N supply in culture medium. In both T. lutea strains, microalgal final cell density was significantly lower in N-reduced medium. Carbohydrates...
Tipo: Text
Palavras-chave: Tisochrysis lutea; Nitrogen limitation; Growth phase; Flow cytometry; Fatty acids; Lipid classes.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00399/51074/51936.pdf

Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Guevelou, Eric; Huvet, Arnaud; Galindo-sanchez, Clara E.; Milan, Massimo; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte.
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the...
Tipo: Text
Palavras-chave: AMP-activated protein kinase; Crassostrea gigas; Gametogenesis; Marine bivalve; Reproduction.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00168/27911/26470.pdf

Episodes de mortalité massive de moules bleues observés en 2014 dans les Pertuis charentais
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garrigues, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre; Renault, Tristan.
Mass mortality outbreaks (90-100%) in both juvenile and adult blue mussels were reported in 2014 along the Pertuis Charentais area of the French Atlantic coast. A series of studies were carried out to learn more about the phenomenon through detecting pathogens, describing environmental conditions and establishing the physiological status of the animals. Bacteria identif ied as belonging to the V. splendidus species were detected in dying mussels and seem able to cause mortality in blue mussels in the laboratory. Unusual environmental conditions (large amounts of fresh water, re-suspension of sediments during a series of storms, and a high renewal rate of specific water masses in Brittany’s Pertuis area) may be partially responsible for sparking the phenomen
Tipo: Text
Palavras-chave: Mortalités; Moules; Pertuis Charentais; Vibrio splendidus; Environnement; Mortality; Mussels; Pertuis Charentais; Vibrio splendidus; Environment.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00312/42343/41682.pdf

Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Da Costa Gonzalez, Fiz; Robert, Rene; Quere, Claudie; Wikfors, Gary H.; Soudant, Philippe.
Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5...
Tipo: Text
Palavras-chave: Fatty acid; Larvae; Lipids; Metabolism; Oyster; Synthesis.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00268/37962/36086.pdf

Seasonal variation of sperm quality in Pacific oyster (Crassostrea gigas)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Malo, Florent; Queau, Isabelle; Ratiskol, Dominique; Quere, Claudie; Le Grand, Jacqueline; Fauvel, Christian.
Seasonal changes of sperm quality which can affect sperm biological parameters throughout the breeding period, have been little studied in mollusc species. Controlling gamete quality would aid the management of gametes in hatcheries and the development of selection programs. The aim of the present study was to describe the changes in sperm quality of wild Pacific oysters through the spawning season by comparing sperm parameters at the beginning (May), middle (July) and end (October) of this period using a panel of bio-descriptors. These parameters were studied over the 2014 breeding season based on shed sperm collected after serotonin injection of wild breeders. A significantly higher percentage of motile sperm was observed at the end of the spawning...
Tipo: Text
Palavras-chave: Crassostrea gigas; Sperm quality; Sperm ageing; Season; Motility.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00346/45690/45310.pdf

Surmortalités de la moule bleue Mytilus edulis dans les Pertuis Charentais (mars 2014)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Renault, Tristan; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garriques, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre.
Suite aux surmortalités observées au sein des élevages de moules touchant à la fois les animaux adultes et les juvéniles au mois de mars, dans les Pertuis Charentais, la Direction Générale de l’Alimentation (DGAl) a adressé une saisine à Ifremer le 04 avril 2014. Celle-ci fait état d’une demande d’appui scientifique et technique d’Ifremer pour tenter d’apporter des éléments de réponse par rapport à cette mortalité subite et importante. L’Ifremer a proposé un projet d’action permettant l’expertise du phénomène de surmortalité de la moule bleue, selon 3 axes : l’impact environnemental, la pathologie et les caractéristiques physiologiques des moules. Il s’agit de mettre en évidence la présence ou non d’agents infectieux connus ou émergents, d’apporter des...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00229/34022/32387.pdf

Mortalité du naissain d’Huître creuse Crassostrea gigas dans l’étang de Thau en 2009
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Malet, Nathalie; Pastoureaud, Annie; Munaron, Dominique; De Lorgeril, Julien; Bachere, Evelyne; Vaquer, Andre; Huvet, Arnaud; Corporeau, Charlotte; Normand, Julien; Boudry, Pierre; Moal, Jeanne; Quere, Claudie; Quilien, Virgile; Daniel, Jean-yves; Pepin, Jean-francois; Saulnier, Denis; Gonzalez, Jean-louis.
This study was conducted in the Mediterranean area during a mass mortality event of Pacific oyster Crassostrea gigas. The objectives of this work were to increase our understanding of the mass mortality phenomena in Pacific oysters, to identify risky cultivation practises and to propose mitigation strategies. In the first part of this work, we followed environmental parameters of the Thau lagoon in relation with mortality events. Then, we compared mortality and growth parameters of oysters as a function of their origins, their development stages and cultivation sites in relation with energetic, reproduction and pathogens. Finally, we conducted transfer experiments where oysters maintained free of mortality in open sea were transferred in the Thau lagoon...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11354/7927.pdf

Functional capacities of gill mitochondria in oyster Crassostrea gigas during an emersion/immersion tidal cycle
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Dudognon, Tony; Soudant, Philippe; Seguineau, Catherine; Quere, Claudie; Auffret, Michel; Kraffe, Edouard.
Sessile animals that live on the foreshore undergo tidal cycles, and have to face variations in physical and chemical parameters such as oxygen concentration. During emersion, availability of dissolved oxygen can be lowered for bivalves, which have only a small reserve of seawater inside their closed shell. Differences in oxygen concentration are thus expected to lead to modifications of the metabolism, including changes in mitochondrial activity. Previous studies investigated air exposure under extreme conditions, which do not always reflect environmental conditions these invertebrates have to cope with. In this study, oxidative capacities of gill mitochondria of the oyster Crassostrea gigas were studied during a tidal cycle period, by comparing oysters...
Tipo: Text
Palavras-chave: Mitochondria; Oxygen consumption; ATP production; Respiratory chain inhibitor; Crassostrea gigas.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26854/24981.pdf

Spatial and Temporal Dynamics of Mass Mortalities in Oysters Is Influenced by Energetic Reserves and Food Quality
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pernet, Fabrice; Lagarde, Franck; Jeannee, Nicolas; Daigle, Gaetan; Barret, Jean; Le Gall, Patrik; Quere, Claudie; Roque D'Orbcastel, Emmanuelle.
Although spatial studies of diseases on land have a long history, far fewer have been made on aquatic diseases. Here, we present the first large-scale, high-resolution spatial and temporal representation of a mass mortality phenomenon cause by the Ostreid herpesvirus (OsHV-1) that has affected oysters (Crassostrea gigas) every year since 2008, in relation to their energetic reserves and the quality of their food. Disease mortality was investigated in healthy oysters deployed at 106 locations in the Thau Mediterranean lagoon before the start of the epizootic in spring 2011. We found that disease mortality of oysters showed strong spatial dependence clearly reflecting the epizootic process of local transmission. Disease initiated inside oyster farms spread...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00181/29249/27663.pdf

Physiological changes in Pacific oyster Crassostrea gigas exposed to the herpesvirus OsHV-1 μvar
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Tamayo, David; Corporeau, Charlotte; Petton, Bruno; Quere, Claudie; Pernet, Fabrice.
Since 2008, mass mortality events of Crassostrea gigas have been occurring along the French coast when seawater temperature exceeds 16ºC. These mortality events are related to a particular genotype of the ostreid herpesvirus named OsHV-1 μvar. The present study aimed to detail various physiological aspects underlying the onset of the disease. In the laboratory, both exposed (infected) and naïve (healthy) oysters were maintained at 13.0°C and 20.6ºC. These temperatures were respectively lower and higher than the threshold values of 16°C at which the disease generally occur. At 20.6ºC, exposed oysters were characterized by a reduction in energetic reserves (carbohydrates and triglycerides) together with a decrease in protein content. Sterols levels were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Energetic reserves; Energetic metabolism; Enzyme activity; OsHV-1; Temperature.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00192/30330/28803.pdf

Gamete quality in triploid Pacific oyster (Crassostrea gigas)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Malo, Florent; Quere, Claudie; Ledu, Christophe; Le Grand, Jacqueline; Benabdelmouna, Abdellah.
Triploidy induction in shellfish aims to obtain faster growth and sterility of reared individuals. Triploid Pacific oysters are most often not totally sterile, but have greatly reduced reproductive capacities compared to those recorded in diploid individuals. The description of gamete output in triploid aquatic animals is most often confined to the assessment of fecundity in females. The present work aims at further describing gamete quality of triploid Pacific oysters and comparing it to values observed in diploid individuals. Diploid and triploid oysters (produced by mating tetraploid males × diploid females) were reared in standard conditions and then transferred to the wild to allow the offspring to attain reproductive maturity. At the end of...
Tipo: Text
Palavras-chave: Crassostrea gigas; Reproduction; Triploidy; Gamete.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00277/38835/37581.pdf

Effect of Rhodomonas salina addition to a standard hatchery diet during the early ontogeny of the scallop Pecten maximus
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Tremblay, Rejean; Cartier, Simon; Miner, Philippe; Pernet, Fabrice; Quere, Claudie; Moal, Jeanne; Muzellec, Marie-louise; Mazuret, Michel; Samain, Jean-francois.
The main objective of this study was to identify algal diets that maximize the survival and growth and alter the biochemical content of Pecten maximus larvae with the aim of improving metamorphosis. We also evaluated the potential of the cryptophyceae Rhodomonas salina as a food source for these larvae. Two flagellates, Isochrysis aff. galbana (T) and Pavlova lutheri (P), and two diatoms, Chaetoceros gracilis (C) or Skeletonema costatum (S), were tested as two ternary diets, namely PTC and PTS. PTC and PTS were compared with diets that also included R. salina (R). The addition of R. salina and the replacement of C. gracilis by S. costatum in the traditional hatchery diet seem to be two interesting alternatives for increasing the productivity of larval...
Tipo: Text
Palavras-chave: Algal fatty acids and sterols; Energetic content; Metamorphosis; Nutrition; Scallop.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2404.pdf

Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Corporeau, Charlotte; Vanderplancke, Gwenaelle; Boulais, Myrina; Suquet, Marc; Quere, Claudie; Boudry, Pierre; Huvet, Arnaud; Madec, Stephanie.
We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin — breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and...
Tipo: Text
Palavras-chave: Marine bivalves; Crassostrea gigas; Oocyte quality; Proteomics.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20925/18534.pdf

The quality of great scallop (Pecten maximus) sperm after thawing
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Gourtay, Clemence; Donval, Anne; Le Goic, Nelly; Quere, Claudie; Malo, Florent; Le Grand, Jacqueline; Ratiskol, Dominique; Mingant, Christian; Fauvel, Christian.
Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm samples were obtained after intragonadal injection of serotonin and cryopreserved using a previously published protocol.  Sperm quality was assessed using a panel of four parameters: sperm motility characteristics, using a a computer assisted sperm analysis plugin with Image J, intracellular ATP content using an ATP-Lite kit, sperm integrity, using flow cytometry and sperm...
Tipo: Text
Palavras-chave: Pecten maximus; Reproduction; Gamete; Sperm; Cryopreservation.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00317/42862/43912.pdf

ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas )
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc.
Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability...
Tipo: Text
Palavras-chave: Spermatozoa; Quality; Motility; Viability; ATP content; ROS production.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00386/49769/50309.pdf

The selection of an ideal diet for Ostrea edulis (L.) broodstock conditioning (part B)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gonzalez-araya, Ricardo; Lebrun, Luc; Quere, Claudie; Robert, Rene.
Four microalgae species (Rhodomonas salina, Thalassiosira weissflogii, Thalassiosira pseudonana and Pavlova lutheri) were evaluated to estimate their potential as food for Ostrea edulis (L.) reproductive conditioning. Best ingestion and absorption were observed with R. salina (3.44 and 1.59 mg g− 1 h− 1, respectively), followed by T. pseudonana (2.75 and 0.98 mg g− 1 h− 1) and P. lutheri (2.40 and 0.91 mg g− 1 h− 1). Oysters fed T. weissflogii exhibited the lowest ingestion and absorption values (1.40 and 0.68 mg g− 1 h− 1). Proximate composition (proteins and carbohydrates) and lipid content (fatty acids and sterols) analysed in four main tissues (gonad, digestive gland, muscle and gills) also differed significantly with diet. Protein ranged from 355 mg...
Tipo: Text
Palavras-chave: Ostrea edulis; Conditioning; Algal diets; Ingestion; Absorption; Biochemical composition.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00090/20092/17731.pdf

Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Da Costa, F.; Petton, Bruno; Mingant, Christian; Bougaran, Gael; Rouxel, Catherine; Quere, Claudie; Wikfors, G. H.; Soudant, P.; Robert, Rene.
Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93–95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31–33%) and total fatty acids (4.0–4.7 pg cell−1). Larval oyster survival and growth rate...
Tipo: Text
Palavras-chave: Larvae; Lipids; Oyster; Tisochrysis lutea.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00251/36258/36063.pdf

Trophic niche of two co-occurring ophiuroid species in impacted coastal systems, derived from fatty acid and stable isotope analyses
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Blanchet-aurigny, Aline; Dubois, Stanislas; Quere, Claudie; Guillou, Monique; Pernet, Fabrice.
The trophic niches of 2 common co-occurring ophiuroids, Ophiocomina nigra and Ophiothrix fragilis (Echinodermata, Ophiuroidea), in 2 contrasting coastal systems of Brittany (France) were investigated. We used a combination of fatty acid biomarkers derived from neutral lipids and stable isotopic compositions to explore the contributions of oceanic versus continental inputs to the ophiuroids' diet. We investigated 2 different systems with an inshore versus offshore comparison. We sampled potential food sources and surveyed organisms every 2 mo for 1 yr. Spatio-temporal variations in stable isotopes and fatty acid profiles of the ophiuroids were generally low compared to interspecific differences. Fatty acid markers showed that both ophiuroids relied on...
Tipo: Text
Palavras-chave: Echinoderm; Feeding ecology; Ophiocomina nigra; Ophiothrix fragilis; Trophic markers; Green algae.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00270/38144/36278.pdf

Mortalités massives de l’Huître creuse: causes et perspectives
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Lagarde, Franck; Fiandrino, Annie; Huvet, Arnaud; Corporeau, Charlotte; Boudry, Pierre; Quere, Claudie; Degremont, Lionel; Pepin, Jean-francois; Saulnier, Denis; Boulet, Hélène; Keck, Nicolas.
The objectives of this study are to increase our understanding of the mass mortality phenomena in Pacific oysters Crassostrea gigas in the Thau lagoon and to examine the effect of cultivation practises. Mortality events in 2009 and 2010 always coincide with simple or dual infections involving primarily the Herpes virus OsHV-I and secondarily the bacteria Vibrio splendidus, when seawater temperature is between 17°C and 24°C. In the Thau lagoon, mortality starts early May and last until fall with an interruption period when seawater temperature is higher than 24°C in July and August. Mortality of oysters varies as a function of zootechnical life-history, hydrodynamics and cultivation practises. First, mortality depends on whether or not oysters have been...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00043/15404/12764.pdf

Low pH reduced survival of the oyster Crassostrea gigas exposed to the Ostreid herpesvirus 1 by altering the metabolic response of the host
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Fuhrmann, Marine; Richard, Gaëlle; Quere, Claudie; Petton, Bruno; Pernet, Fabrice.
Environmental change in the marine realm has been accompanied by emerging diseases as new pathogens evolve to take advantage of hosts weakened by environmental stress. Here we investigated how an exposure to reduced seawater pH influenced the response of the oyster Crassostrea gigas to an infection by the Ostreid herpesvirus type I (OsHV-1). Oysters were acclimated at pH 8.1 or pH 7.8 and then exposed to OsHV-1. Their survival was monitored and oyster tissues were sampled for biochemical analyses. The survival of oysters exposed to OsHV-1 at pH 7.8 was lower (33.5%) than that of their counterparts at pH 8.1 (44.8%) whereas levels of OsHV-1 DNA were similar. Energetic reserves, fatty acid composition and prostaglandin levels in oyster did not vary...
Tipo: Text
Palavras-chave: Acidification; Bivalve; Disease; Environment; Metabolism; Mortality risk.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00472/58334/60887.pdf

Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boulais, Myrina; Corporeau, Charlotte; Huvet, Arnaud; Bernard, Ismael; Quere, Claudie; Quillien, Virgile; Fabioux, Caroline; Suquet, Marc.
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oocyte quality; Trochophore; Lipid; Gene expression; ATP.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00239/35010/33544.pdf

Effect of nutrition on Crassostrea gigas larval development and the evolution of physiological indices Part B: Effects of temporary food deprivation
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ben Kheder, Rym; Quere, Claudie; Moal, Jeanne; Robert, Rene.
In the natural environment bivalve larvae are exposed to variable conditions and can therefore face periods of food shortage. To understand the resistance of early life stages of Pacific oyster (Crassostrea gigas), larvae were experimentally starved for 4 days at different periods of their development, and the resulting variations in their lipid reserves then analysed using image analysis and biochemical techniques. Faced with a temporary lack of food, the larvae halted their growth in size and weight and started to live off their lipid reserves; this effect was highly marked when the period of food deprivation was at the beginning of larval rearing, but was less marked when food deprivation was after day 14. The larvae conserved their developmental...
Tipo: Text
Palavras-chave: Crassostrea gigas; Larvae; Growth; Metamorphosis; Starvation; Lipids.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00018/12906/10145.pdf

An amylase gene polymorphism is associated with growth differences in the Pacific cupped oyster Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Prudence, Marie; Moal, Jeanne; Boudry, Pierre; Daniel, Jean-yves; Quere, Claudie; Jeffroy, Fanny; Mingant, Christian; Ropert, Michel; Bedier, Edouard; Van Wormhoudt, A; Samain, Jean-francois; Huvet, Arnaud.
This study investigated the non-neutrality of genetic polymorphism in two alpha-amylase genes (AMYA and AMYB) in the oyster Crassostrea gigas. Bi-parental oyster families, bred to be polymorphic for markers in these genes, were monitored for growth and survival for 1 year under standard culture conditions in two French production sites. Within-family genotype frequencies indicated that the two amylase genes were closely linked (c. 1.7 cM). Within two of three families, significant differences in growth were observed between genotypes at one of the two production sites, suggesting that this polymorphism is not neutral and might be under selection because of its role in digestive function. Estimated daily yields were different between amylase genotypes,...
Tipo: Text
Palavras-chave: Growth; Genotype; Digestive enzyme; Bivalve; Alpha amylase.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1691.pdf

Amélioration des productions phytoplanctoniques en écloserie de mollusques : caractérisation des microalgues fourrage
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Robert, Rene; Chretiennot-dinet, Marie-josèphe; Kaas, Raymond; Martin-jezequel, Véronique; Moal, Jeanne; Le Coz, Jean-rene; Nicolas, Jean-louis; Bernard, Eudes; Connan, Jean-paul; Le Dean, Loic; Le Gourrierec, Gaetane; Leroy, Bertrand; Quere, Claudie.
in mollusc hatcheries. Consequently, we assessed themost commonly used species to establish their growth and size profiles, their cytomorphological, biochemical, biomolecular identification as well as their cytometer signs, and also their ability to grow under extreme conditions of temperature and salinity. This work aimed at matching the conformity of the strains used in Argenton (dispatched to most of the French commercial hatcheries) to those of a referenced culture collection. A previous screening preceded this study, because of the 45 species and/or clones held originally, Argenton kept no more than 15 species. As most of the latter are also represented in referenced collection, our strains are therefore correctly identified. On the other hand, the...
Tipo: Text
Palavras-chave: Ecological limits; Biomolecular profile; Flow cytometry; Biochemical composition; Cytomorphology; Phytoplankton; Mollusc; Hatchery; Ecotolérance; Profil biomoléculaire; Cytométrie en flux; Composition biochimique; Cytomorphologie; Phytoplancton; Mollusques; Ecloserie.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/rapport-1546.pdf

Changes in motility, ATP content, morphology and fertilisation capacity during the movement phase of tetraploid Pacific oyster (Crassostrea gigas) sperm
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Suquet, Marc; Labbe, C.; Brizard, Raphael; Donval, Anne; Le Coz, Jean-rene; Quere, Claudie; Haffray, P.
Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected on three tetraploid males were activated in seawater. Their features were analysed over a 24 h period and compared to a sperm pool collected on three diploid males as a reference. The percentage of motile spermatozoa, the intracellular ATP content, and the fine structure of spermatozoa were studied in relation to time post activation. Furthermore, the fertilisation...
Tipo: Text
Palavras-chave: Crassostrea gigas; Sperm; Tetraploid; Motility; ATP; Ultrastructure.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00006/11756/8639.pdf

Tissue expression of two alpha-amylase genes in the Pacific oyster Crassostrea gigas. Effects of two different food rations
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Huvet, Arnaud; Daniel, Jean-yves; Quere, Claudie; Dubois, Solene; Prudence, Marie; Van Wormhoudt, A; Sellos, D; Samain, Jean-francois; Moal, Jeanne.
A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to estimate tissue-specific expression of two amylase genes in five different tissues of the Pacific oyster Crassostrea gigas and the experimental effects of food level on the cellular, enzymatic and gene expression changes of the digestive gland. Results indicated that both genes were transcribed at different levels depending on tissue type. A strong preferential expression of both amylase genes was observed in the digestive gland leading to high amylase activities, in accordance with the digestive function of this organ. Traces of gene expression and activity were observed in other analysed tissues. These appeared higher in labial palps than in other...
Tipo: Text
Palavras-chave: Oyster; Gene expression; Food regulation; Digestive enzyme activity; Alpha amylase.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-394.pdf

The combined effects of blue light and dilution rate on lipid class and fatty acid composition of Tisochrysis lutea
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Marchetti, Julie; Da Costa, Fiz; Bougaran, Gael; Quere, Claudie; Soudant, Philippe; Robert, Rene.
The lipid class and the fatty acid compositions of microalgae highly influence bivalve larval and post-larval development. Light is an essential environmental factor for microalgal culture, and quantity and quality of light may induce changes in the biochemical composition of the algae. The objective of this study was to investigate the effect of light spectrum (blue vs. white light) on lipid class and fatty acid compositions of Tisochrysis lutea cultured in a chemostat. Two different dilution rates (D) were assayed for each light spectrum: 0.2 and 0.7 day−1. Triacylglycerol (TAG), sterol, and hydrocarbon (HC) content increased sharply at low D. The proportion of alkenones was significantly reduced under blue light. Polyunsaturated fatty acids (PUFA), and...
Tipo: Text
Palavras-chave: Tisochrysis lutea; Blue light; Dilution rate; Lipid classes; Fatty acids; Chemostat.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00412/52299/53355.pdf

Effect of a mono-specific algal diet on immune functions in two bivalve species - Crassostrea gigas and Ruditapes philippinarum
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois.
The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte...
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf

A physiological and biochemical approach to selecting the ideal diet for Ostrea edulis (L.) broodstock conditioning (part A)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gonzalez-araya, Ricardo; Queau, Isabelle; Quere, Claudie; Moal, Jeanne; Robert, Rene.
To select the best microalgae for Ostrea edulis conditioning, four single species diets were fed to batches of broodstock, which were then compared using physiological and biochemical approaches. Ingestion and absorption were first studied, showing that Chaetoceros gracilis and Skeletonema marinoï were efficiently ingested (4.9–5.3 mg g−1 h−1) and absorbed (1.9–2.5 mg g−1 h−1) while Tetraselmis suecica led to the lowest physiological responses (0.36 and 0.12 mg g−1 h−1 respectively). When O. edulis were fed any microalgae except T-ISO with only 79 mg g−1, gonads accumulated carbohydrates from 116 to 141 mg g−1 and the extent of accumulation depended on the species supplied in the order T. suecica&lt;C. gracilis&lt;S. marinoï. When oysters were fed either...
Tipo: Text
Palavras-chave: Ostrea edulis; Conditioning; Algal diets; Ingestion; Absorption; Biochemical composition.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14449/11748.pdf

Effect of nutrition on Crassostrea gigas larval development and the evolution of physiological indices. Part A: Quantitative and qualitative diet effects
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ben Kheder, Rym; Quere, Claudie; Moal, Jeanne; Robert, Rene.
Tipo: Text
Palavras-chave: Crassostrea gigas; Larvae; Growth; Metamorphosis; Nutrition level and quality; Lipids; Condition indices.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00009/11997/9041.pdf

The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Delisle, Lizenn; Fuhrmann, Marine; Quere, Claudie; Pauletto, Marianna; Pichereau, Vianney; Pernet, Fabrice; Corporeau, Charlotte.
Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in...
Tipo: Text
Palavras-chave: Voltage-dependent anion channel; Warburg effect; Crassostrea gigas; Ostreid herpes virus.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00420/53129/54142.pdf

Marine diatoms sustain growth of bivalves in a Mediterranean lagoon
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pernet, Fabrice; Malet, Nathalie; Pastoureaud, Annie; Vaquer, Andre; Quere, Claudie; Dubroca, Laurent.
Carbon stable isotopes and fatty acids were measured in the suspended particulate organic matter (POM) of the Thau lagoon to study its qualitative temporal changes in relation to environmental factors and to identify the food sources of bivalves over a one-yr-cycle in relation to their growth. Reciprocally, the impact of shellfish farming on POM was also studied. Oysters and mussels were sampled and measured for biometry, stable isotopes and fatty acid composition. Water samples were collected at two sites, both inside and outside of the shellfish farming area, to determine concentrations in POM, chlorophyll a (Chl a) and stable isotopes. Carbon isotopes and fatty acids in bivalves reflected seasonal changes in food sources, which varied consistently with...
Tipo: Text
Palavras-chave: Aquaculture; Bivalve; Competition; Feeding ecology; Growth; Trophic markers.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00075/18575/16177.pdf