Consulta: creatorFacets:"ROSEMBLATT,MARIO"
Registros recuperados: 7
Data/hora: 30/05/2024 14:22:31

XVI Reunión Anual de la Sociedad de Biología Celular de Chile
Provedor de dados: 48
Autores: ROSEMBLATT,MARIO.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300005

Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis
Provedor de dados: 48
Autores: WILHELM,VIVIAN; HUARACÁN,BERNARDO; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D.T.
The genes encoding the heat shock proteins HSP10 and HSP16 of the salmon pathogen Piscirickettsia salmonis have been isolated and sequenced. The HSP10 coding sequence is located in an open reading frame of 291 base pairs encoding 96 aminoacids. The HSP16 coding region was isolated as a 471 base pair fragment encoding a protein of 156 aminoacids. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from other prokaryotic organisms. Both proteins were expressed in E. coli as fusion proteins with thioredoxin and purified by chromatography on Ni-column. A rabbit serum against P. salmonis total proteins reacts with the recombinant HSP10 and HSP16 proteins. Similar reactivity was determined by ELISA using serum...
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300013

Complete sequence of the genome of the human isolate of Andes virus CHI-7913: comparative sequence and protein structure analysis
Provedor de dados: 48
Autores: TISCHLER,NICOLE D; FERNÁNDEZ,JORGE; MÜLLER,ILSE; MARTÍNEZ,RODRIGO; GALENO,HÉCTOR; VILLAGRA,ELIECER; MORA,JUDITH; RAMÍREZ,EUGENIO; ROSEMBLATT,MARIO; VALENZUELA,PABLO D.T.
We report here the complete genomic sequence of the Chilean human isolate of Andes virus CHI-7913. The S, M, and L genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall GC content of 38.7%. These genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a RNA-dependent RNA polymerase of 2,152 amino acids. In addition, the genome also has other ORFs coding for putative proteins of 34 to 103 amino acids. The encoded proteins have greater than 98% overall similarity with the proteins of Andes virus isolates AH-1 and Chile R123. Among other sequenced Hantavirus, CHI-7913 is more closely related to Sin Nombre virus, with an overall protein...
Tipo: Journal article
Palavras-chave: Hantavirus; Genome sequence; RNA polymerase; Human isolate.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200010

CHILE INVITA A CHILE: INSTITUTO MILENIO DE BIOLOGIA FUNDAMENTAL Y APLICADA ORGANIZA ORIGINAL ENCUENTRO DE ESTUDIANTES
Provedor de dados: 48
Autores: ROSEMBLATT,MARIO.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200007

Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
Provedor de dados: 48
Autores: WILHELM,VIVIAN; SOZA,CRISTIÁN; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D.T.
We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective...
Tipo: Journal article
Palavras-chave: Piscirickettsia salmonis; Hsp60; Hsp70; GroEL; DnaK; Immune response.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100009

The Bacteriophage lambdaDNA packaging enzyme: Identification of four structural domains of the gpNu1 subunit using limited proteolysis
Provedor de dados: 48
Autores: ARAYA,PAMELA; ROSEMBLATT,MARIO; VALENZUELA,PABLO; MURIALDO,HELIOS.
Lambda DNA terminase, the enzyme that cleaves virion-length chromosomes from multigenomic concatemers and packages them into the bacteriophage head, is composed of two subunits, gpNu1 and gpA. Direct determination of the structure of gpNu1, the smaller subunit, has not been possible because of its insolubility in aqueous solutions. Therefore, to identify smaller and potentially water-soluble domains of gpNu1, we analyzed the nature of the products obtained by limited digestion of the protein with several proteases. The gpNu1 subunit was obtained from E.coli cells transfected with the plasmid pH6-Nu1 that overproduces the protein. Incubation of gpNu1 solubized in 2.5 M guanidinium chloride with chymotrypsin resulted in the formation of at least eight...
Tipo: Journal article
Palavras-chave: DNA packaging; Terminase; GpNu1 structural domains; Limited proteolysis.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300008

Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis
Provedor de dados: 48
Autores: WILHELM,VIVIAN; MORALES,CARMEN; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D. T.
We have isolated and sequenced the genes encoding the membrane bound transglycosylase B (MltB) and the transferring binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis. The results of the sequence revealed two open reading frames that encode proteins with calculated molecular weights of 38,830 and 85,140. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from phylogenetically related microorganisms. Partial sequences coding the amino and carboxyl regions of MltB and a sequence of 761 base pairs encoding the amino region of TbpB have been expressed in E. coli. The strong humoral response elicited by these proteins in mouse confirmed the immunogenic properties of the recombinant...
Tipo: Journal article
Palavras-chave: Piscirickettsia salmonis; MltB; TbpB; Recombinant antigens; Recombinant vaccines.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500008