Consulta: creatorFacets:"Ranjbaran,Reza"
Registros recuperados: 1
Data/hora: 10/06/2024 16:24:00

Polyomaviruses BK and JC DNA infection in peripheral blood cells from blood donors
Provedor de dados: 55
Autores: Haghighi,Malihe Fathi; Seyyedi,Noorossadat; Farhadi,Ali; Zare,Farahnaz; Kasraian,Leila; Dehbidi,Gholam Reza Refiei; Ranjbaran,Reza; Behzad-Behbahani,Abbas.
ABSTRACT  Objectives: To investigate the prevalence of human polyomavirus (BK and JC viruses) infection in peripheral blood mononuclear cells of healthy blood donors.  Methods: The study included 250 healthy blood donors. Five-milliliter blood was drawn into sterile EDTA tubes and PBMCs were isolated from whole blood. The isolated PBMCs were counted and stored at −70 °C for future investigation. DNA was extracted and subjected to simple, sensitive and specific semi-nested PCR as well as QPCR using both general and specific primers for different assays.  Results: Of 250 blood samples, 66 (26.4%) were positive for BKV DNA (146-34,514 copies/106 cells). JC DNA was found in 45 (18%) blood samples (65-21,250 copies/106 cells). Co-infection with these viruses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood donors; Leukocytes; Mononuclear; Polyomavirus; Real-time polymerase chain reaction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100022