Consulta: creatorFacets:"Rebouças,N.A."
Registros recuperados: 3
Data/hora: 02/06/2024 08:25:44

Gender differences in vascular expression of endothelin and ET A/ET B receptors, but not in calcium handling mechanisms, in deoxycorticosterone acetate-salt hypertension
Provedor de dados: 56
Autores: David,F.L.; Montezano,A.C.I.; Rebouças,N.A.; Nigro,D.; Fortes,Z.B.; Carvalho,M.H.C.; Tostes,R.C.A.
We determined if the increased vascular responsiveness to endothelin-1 (ET-1) observed in male, but not in female, DOCA-salt rats is associated with differential vascular mRNA expression of ET-1 and/or ET A/ET B receptors or with functional differences in Ca2+ handling mechanisms by vascular myocytes. Uninephrectomized male and female Wistar rats received DOCA and drinking water containing NaCl/KCl. Control rats received vehicle and tap water. Blood pressure and contractile responses of endothelium-denuded aortic rings to agents which induce Ca2+ influx and/or its release from internal stores were measured using standard procedures. Expression of mRNA for ET-1 and ET A/ET B receptors was evaluated by RT-PCR after isolation of total cell RNA from both aorta...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DOCA-salt hypertension; Endothelin-1; Gender; Endothelin receptors; Calcium; Rats.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900006

Identification of clonally rearranged T-cell receptor beta chain genes in HTLV-I carriers as a potential instrument for early detection of neoplasia
Provedor de dados: 56
Autores: Sales,M.M.; Bezerra,C.N.A.; Hiraki,Y.; Melo,N.B.; Rebouças,N.A.
We analyzed the genetic recombination pattern of the T-cell receptor beta-chain gene (TCR-beta) in order to identify clonal expansion of T-lymphocytes in 17 human T-lymphotropic virus type I (HTLV-I)-positive healthy carriers, 7 of them with abnormal features in the peripheral blood lymphocytes. Monoclonal or oligoclonal expansion of T-cells was detected in 5 of 7 HTLV-I-positive patients with abnormal lymphocytes and unconfirmed diagnosis by using PCR amplification of segments of TCR-beta gene, in a set of reactions that target 102 different variable (V) segments, covering all members of the 24 V families available in the gene bank, including the more recently identified segments of the Vbeta-5 and Vbeta-8 family and the two diversity beta segments....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human T-lymphotropic virus 1; Leukemia; Oligoclonal bands; T-cell receptor beta chain; Polymerase chain; Reaction; Adult T-cell leukemia/lymphoma.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500006

Essential regulatory elements for NHE3 gene transcription in renal proximal tubule cells
Provedor de dados: 56
Autores: Neri,E.A.; Bezerra,C.N.A.; Rebouças,N.A.
The objectives of the present study were to identify the cis-elements of the promoter absolutely required for the efficient rat NHE3 gene transcription and to locate positive and negative regulatory elements in the 5’-flanking sequence (5’FS), which might modulate the gene expression in proximal tubules, and to compare this result to those reported for intestinal cell lines. We analyzed the promoter activity of different 5’FS segments of the rat NHE3 gene, in the OKP renal proximal tubule cell line by measuring the activity of the reporter gene luciferase. Because the segment spanning the first 157 bp of 5’FS was the most active it was studied in more detail by sequential deletions, point mutations, and gel shift assays. The essential elements for gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Na+/H+ exchanger isoform 3; Gene promoter; Specificity protein 1; Early growth response factor 1; Proximal tubules.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600004