Consulta: creatorFacets:"Recco-Pimentel,Shirlei M."
Registros recuperados: 8
Data/hora: 17/06/2024 02:18:46

Genetic diversity of Morato's Digger Toad, Proceratophrys moratoi: spatial structure, gene flow, effective size and the need for differential management strategies of populations
Provedor de dados: Genetics and Molecular Biology
Autores: Arruda,Mauricio P.; Costa,William P.; Recco-Pimentel,Shirlei M.
Abstract The Morato's Digger Toad, Proceratophrys moratoi, is a critically endangered toad species with a marked population decline in southern Brazilian Cerrado. Despite this, new populations are being discovered, primarily in the northern part of the distribution range, which raises a number of questions with regard to the conservation status of the species. The present study analyzed the genetic diversity of the species based on microsatellite markers. Our findings permitted the identification of two distinct management units. We found profound genetic structuring between the southern populations, on the left margin of the Tietê River, and all other populations. A marked reduction was observed in the contemporary gene flow among the central populations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proceratophrys moratoi; Microsatellite; Population structure; Management unit; Conservation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300502

Non-random distribution of microsatellite motifs and (TTAGGG)n repeats in the monkey frog Pithecopus rusticus (Anura, Phyllomedusidae) karyotype
Provedor de dados: Genetics and Molecular Biology
Autores: Ernetti,Julia R.; Gazolla,Camilla B.; Recco-Pimentel,Shirlei M.; Luca,Elaine M.; Bruschi,Daniel P.
Abstract The monkey frog, Pithecopus rusticus (Anura, Phyllomedusidae) is endemic to the grasslands of the Araucarias Plateau, southern Brazil. This species is known only from a small population found at the type locality. Here, we analyzed for the first time the chromosomal organization of the repetitive sequences, including seven microsatellite repeats and telomeric sequences (TTAGGG)n in the karyotype of the species by Fluorescence in situ Hybridization. The dinucleotide motifs had a pattern of distribution clearly distinct from those of the tri- and tetranucleotides. The dinucleotide motifs are abundant and widely distributed in the chromosomes, located primarily in the subterminal regions. The tri- and tetranucleotides, by contrast, tend to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphibia; Fluorescence in situ Hybridization; Repetitive DNA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500301

B-chromosomes in two Brazilian populations of Dendropsophus nanus (Anura, Hylidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Medeiros,Lilian R.; Rossa-Feres,Denise C.; Jim,Jorge; Recco-Pimentel,Shirlei M.
We report on the presence of B-chromosomes in two populations of Dendropsophus nanus (= Hyla nana Boulenger, 1889) from São Paulo State, Brazil. Such chromosomes were observed in 4 out of 43 specimens (9.3%) and in 9 out of 15 specimens (60%) from the municipalities of Nova Aliança and Botucatu, respectively. The karyotype 2n = 30 + 1B found in D. nanus was similar to that of other species with 2n = 30 chromosomes, except for the presence of an additional small telocentric chromosome. In one specimen from Botucatu, cells with one to three extra chromosomes were observed. These B-chromosomes appeared as univalent in meiosis I and did not bear a nucleolar organizer region or exhibit constitutive heterochromatin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anura; B-chromosome cytogenetics Dendropsophus supernumerary chromosome.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200010

Restriction fragment analysis of the ribosomal DNA of Paratelmatobius and Scythrophrys species (Anura, Leptodactylidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Lourenço,Luciana B.; Garcia,Paulo C.A.; Recco-Pimentel,Shirlei M.
Physical maps of the ribosomal RNA gene 28S of species belonging to the genera Paratelmatobius and Scythrophrys were constructed, using five restriction endonucleases. The restriction sites for Bam HI, Bgl II, Bst EII, and Eco RI had similar positions in all species, although there were interspecific differences in the size of the restriction fragments obtained. An additional Pvu II site was found in Scythrophrys specimens from Piraquara (State of Paraná, Brazil) and from São Bento do Sul (State of Santa Catarina, Brazil), but not in the Scythrophrys specimens from Rancho Queimado (State of Santa Catarina, Brazil). This finding is in agreement with the hypothesis regarding the existence of two species in the genus Scythrophrys. On the other hand, the extra...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ribosomal DNA; 28S rDNA gene; Restriction sites; Physical map; Paratelmatobius; Scythrophrys; Leptodactylidae; Anura.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200006

The karyotype of three Brazilian Terrarana frogs (Amphibia, Anura) with evidence of a new Barycholos species
Provedor de dados: Genetics and Molecular Biology
Autores: Siqueira,Sérgio; Aguiar Jr.,Odair; Pansonato,André; Giaretta,Ariovaldo A.; Strüssmann,Christine; Martins,Itamar; Recco-Pimentel,Shirlei M.
A recent substantial rearrangement of the 882 described eleutherodactyline frog species has considerably improved the understanding of their systematics. Nevertheless, many taxonomic aspects of the South American eleutherodactyline species remain unknown and require further investigation using morphological, cytogenetic and molecular approaches. In this work, the karyotypes of the Brazilian species Ischnocnema juipoca (Atibaia and Campos do Jordão, SP), Barycholos cf. ternetzi (Uberlândia, MG, and Porto Nacional, TO), and Pristimantis crepitans (Chapada dos Guimarães and São Vicente, MT) were analyzed using Giemsa staining, Ag-NOR labeling, and C-banding techniques. All individuals had a diploid number of 22 chromosomes, but the Fundamental Numbers were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Barycholos; Ischnocnema; Pristimantis; Ag-NOR; C-banding.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300008

Karyotypic characterization of Ramphastidae (Piciformes, Aves)
Provedor de dados: Genetics and Molecular Biology
Autores: Castro,Márcio Siqueira; Recco-Pimentel,Shirlei M.; Rocha,Guaracy Tadeu.
The karyotypes of nine species of the family Ramphastidae were determined and compared with that of the toco toucan (Ramphastos toco), the only ramphastid karyotype so far reported in the literature. Differences in the morphology of the largest chromosomes allowed to identify three karyotype groups. The first group included the species R. toco, Baillonius bailloni, Pteroglossus castanotis, P. aracari and Selenidera maculirostris, in which only the first pair of chromosomes was metacentric. The second group included four Ramphastos species (R. dicolorus, R. ariel, R. vitellinus, R. tucanus cuvieri) with two pairs of metacentric macrochromosomes (the first and the seventh). The third group was represented by a single species, A. laminirostris, in which all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotypes; Rhamphastidae; Toucans.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200006

Cytogenetics of three Brazilian species of Eleutherodactylus (Anura, Leptodactylidae) with 22 chromosomes and re-analysis of multiple translocations in E. binotatus
Provedor de dados: Genetics and Molecular Biology
Autores: Siqueira-Jr.,Sérgio; Ananias,Fernando; Recco-Pimentel,Shirlei M.
In this paper, we provide a cytogenetic analysis of Eleutherodactylus guentheri, E. parvus and E. binotatus. All of the species had a diploid chromosomal number of 2n = 22. The karyotypes of E. guentheri and E. parvus were very similar and differed only slightly in the morphology of pair 2. These two species also had an NOR-bearing secondary constriction on the long arms of pair 6. The karyotype of E. binotatus differed from those of E. guentheri and E. parvus in the morphology and size of the chromosomes, in the number of chromosomal arms, in the NOR location (detected on the short arms of pair 1), and in the pattern of heterochromatin. These results reinforce the differences between E. guentheri and E. binotatus and support the existence of two species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eleutherodactylus; Heterochromatin; Karyotype; Nucleolus organizing region (NOR); Reciprocal translocation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300010

A second case of multivalent meiotic configurations in diploid species of Anura
Provedor de dados: Genetics and Molecular Biology
Autores: Lourenço,Luciana Bolsoni; Recco-Pimentel,Shirlei M.; Cardoso,Adão José.
We analyzed the meiotic chromosomes of specimens from the two karyological groups of Physalaemus petersi (Jiménez de la Espada, 1872) described in the literature. Multivalent configurations (rings or chains) were observed in both groups. This meiotic organization resulted from the terminal association of non-homologous chromosomes, and an analysis of C-banded multivalents indicated no involvement of heterochromatic regions in these associations. A possible explanation for such meiotic configurations is the occurrence of heterozygous translocations. Thus, multivalents may indicate the involvement of translocation events in the karyotypic evolution of P. petersi.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100024