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Application of the differential display RT-PCR strategy for the identification of inflammation-related mouse genes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva,A.M.; Pires,E.G.; Abrantes,E.F.; Ferreira,L.R.P.; Gazzinelli,R.T.; Reis,L.F.L.
The inflammatory response elicited by various stimuli such as microbial products or cytokines is determined by differences in the pattern of cellular gene expression. We have used the differential display RT-PCR (DDRT-PCR) strategy to identify mRNAs that are differentially expressed in various murine cell types stimulated with pro-inflammatory cytokines, microbial products or anti-inflammatory drugs. Mouse embryonic fibroblasts (MEFs) were treated with IFNs, TNF, or sodium salicylate. Also, peritoneal macrophages from C3H/Hej mice were stimulated with T. cruzi-derived GPI-mucin and/or IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;. After DDRT-PCR, various cDNA fragments that were differentially represented on the sequencing gel were recovered, cloned and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DDRT-PCR; Gene expression; Inflammation; TNF; Interferon.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700008