Consulta: creatorFacets:"Ribeiro,Marcelo Lima"
Registros recuperados: 3
Data/hora: 09/06/2024 05:33:53

Quantitative real-time PCR for the clinical detection of Helicobacter pylori
Provedor de dados: Genetics and Molecular Biology
Autores: Ribeiro,Marcelo Lima; Ecclissato,Christina Cunha; Mattos,Ricardo Gabriel; Mendonca,Sergio; Pedrazzoli Jr.,José.
Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups: 46 biopsies from untreated patients who according to the references methods were considered H. pylori-negative (group A); 35 biopsies from patients previously treated against H. pylori and considered to be cured by "gold standard" tests (group B); and 10 biopsies from patients H. pylori-positive by all available methods (group C). The sensitivity of the RT-PCR assay was higher than that of standard methods. Of the 81 patients considered to be uninfected according...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnostic methods; Helicobacter pylori; Real-time PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300022

Characterization of virulence genes cagA and vacA in Helicobacter Pylori and their prevalence in gastrointestinal disorders
Provedor de dados: Brazilian Journal of Microbiology
Autores: Cogo,Laura Lúcia; Monteiro,Cristina Leise Bastos; Nogueira,Keite da Silva; Palmeiro,Jussara Kasuko; Ribeiro,Marcelo Lima; Camargo,Eloá Ramalho de; Neves,Daniel Locatelli; Nascimento,Aguinaldo José do; Costa,Libera Maria Dalla.
Prevalence of H. pylori infection was determined using cultures of gastric biopsy samples of patients attended at the academic hospital of the Federal University of Paraná, Curitiba, Paraná, Brazil. Molecular methods were used to characterize the cagA and vacA genes from bacterial isolates associated with different diseases presented by patients. Out of a total of 81, forty-two gastric biopsy samples tested were positive for H. pylori, with a prevalence of 51.9%. No significant difference was found with regard to the gender (p=0.793) and age (p=0.183) of the patients. Genotype s1m1 vacA gene was found in 67% of the cases of peptic ulcer investigated (p=1.0), despite the limited number of patients with this disease (n=3). A correlation between the presence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Virulence genes; CagA; VacA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400007

Anti-Helicobacter pylori activity of plant extracts traditionally used for the treatment of gastrointestinal disorders
Provedor de dados: Brazilian Journal of Microbiology
Autores: Cogo,Laura Lúcia; Monteiro,Cristina Leise Bastos; Miguel,Marilis Dallarmi; Miguel,Obdulio Gomes; Cunico,Miriam Machado; Ribeiro,Marcelo Lima; Camargo,Eloá Ramalho de; Kussen,Gislene Maria Botão; Nogueira,Keite da Silva; Costa,Libera Maria Dalla.
The antibacterial activity of plant extracts obtained from Bixa orellana L., Chamomilla recutita L., Ilex paraguariensis A. St.-Hil., Malva sylvestris L., Plantago major L. and Rheum rhaponticum L. has been evaluated against two reference strains and eleven clinical isolates of Helicobacter pylori. All the plant species chosen are used in popular Brazilian cuisine and folk medicine in the treatment of gastrointestinal disorders. Initial screening was made by the disk diffusion test and then minimum inhibitory concentration was determined by the agar dilution method. The results presented in this work demonstrated that among the plant preparations analyzed, B. orellana L., C. recutita L., I. paraguariensis A. St.-Hil. and M. sylvestris L. were capable of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Antibacterial activity; Plant extracts.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200007