Consulta: creatorFacets:"Ristow,Paula"
Registros recuperados: 6
Data/hora: 02/06/2024 13:40:14

Improvement of an in-house ELISA for bovine paratuberculosis serology in Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Marassi,Carla Dray; Fonseca,Leila de Souza; Ristow,Paula; Ferreira,Rachel; Lilenbaum,Walter; Oelemann,Walter M.R.
Paratuberculosis (Johne's disease) is a chronic enteritis in cattle caused by infection with Mycobacterium avium subsp. paratuberculosis (Map). In Brazil, few reports describe the isolation of the organism. The gold standard test for Map is its isolation from tissues or feces. Moreover, bacterial growth is slow and test results are available only after three to five months of incubation Furthermore, shedding of bacilli at levels detectable by fecal culture is irregular and does not occur during the early stages of infection, which compromises the sensitivity of this methodology. The most common immunological tests to identify Map infection are complement fixation test (CFT), agar gel immunodiffusion (AGID) and enzyme-linked immunoassay (ELISA). In Brazil,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Cattle; ELISA; Serology; Diagnosis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200004

Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums
Provedor de dados: Brazilian Journal of Microbiology
Autores: Santos,Ana Amélia Nunes; Figueira,Cláudio Pereira; Reis,Mitermayer Galvão dos; Costa,Federico; Ristow,Paula.
Abstract We evaluated the renal colonization by Leptospira interrogans in Rattus norvegicus (rats), as it is the major natural reservoir of urban leptospirosis. We caught 72 R. norvegicus, out of which 32 were found to be positive for L. interrogans by immunofluorescence assay. From these rats, we selected 17 and divided them into six groups based on the mass-age/sex. We performed the immunohistochemistry test against L. interrogans in the kidney sections of the rats and systematically counted the colonized tubules (CTs) in 20 fields. The proportion of positive fields varied from 5% to 95%. The number of CTs in 20 fields varied from 0.5 to 85.5. These differences were not related to age or sex of the animals. The characterization of leptospiral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira interrogans; Renal colonization; Natural reservoirs; Rattus norvegicus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401161

Molecular characterization of the first leptospires isolated from goats in Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Lilenbaum,Walter; Kremer,Frederico; Ristow,Paula; Dellagostin,Odir; Bourhy,Pascale; Hartskeerl,Rudy; Vasconcellos,Silvio.
Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Serogrouping; Sequencing; PFGE; Rrs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050

An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
Provedor de dados: Brazilian Journal of Microbiology
Autores: Marassi,Carla Dray; Silva,Marley; Oelemann,Walter Martin Roland; Fonseca,Leila de Souza; Ristow,Paula; Lilenbaum,Walter.
ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ &gt; 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacteria; ELISA; M. fortuitum; M. phlei.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300019

Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Marassi,Carla Dray; Gonzaga,Janete da Silva; Ristow,Paula; Ferreira,Rachel; Fonseca,Leila Souza; Oelemann,Walter; Lilenbaum,Walter.
An in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (kappa&gt; 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Mycobacterium avium subsp. paratuberculosis; Serology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100002

Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle
Provedor de dados: Brazilian Journal of Microbiology
Autores: Lilenbaum,Walter; Ferreira,Rachel; Marassi,Carla Dray; Ristow,Paula; Oelemann,Walter Martins Roland; Fonseca,Leila de Souza.
Forty-four cows from five herds infected with tuberculosis (TB) and without paratuberculosis (PTB), and 21 cows from a herd without either infection were studied. The cattle presented concordant results in both the skin test and gamma-interferon assay for TB and two commercial ELISAs for PTB. Animals were divided according to TB test results into Group A with 28 TB-infected animals, Group B with 16 TB-negative animals from infected herds, and Group C with 21 TB-negative cows from a tuberculosis-free herd (which were used as controls). Twenty of 28 animals from Group A (71.4%), 6/16 from Group B (37.5%) and none from Group C were reactive to PTB ELISAs, suggesting that these commercial kits were unable to distinguish between PTB and TB. It is proposed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Tuberculosis; Mycobacterium bovis; Mycobacterium avium paratuberculosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300016